H6621SFIn stock — ships in 48 hrsCell Culture Media

Serum-Free Epithelial Cell Medium /w Kit

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    • Shipped on dry ice overnight within the US, 2-day international
    • CoA per lot on request
    • Performance guarantee replaced free if cells fail to attach

    For research use only. Not for use in diagnostic or therapeutic procedures.

    $125.00
    • ModelNormal
    • Format500 ML

    Description

    Serum-Free Epithelial Cell Medium /w Kit – 500 ML

    Catalog Number: H6621SF

    (500 ml Basal medium with the growth factor supplement)

    Description

    Serum-Free Epithelial Cell Medium is a complete medium designed for the culture of epithelial cells. It was tested and optimized with epithelial cell growth and proliferation in vitro. It is formulated for use with 5% CO2 and 95% air in a humidified incubator.

    The medium consists of 500 ml of basal medium (containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals), supplemented with epithelial cell growth supplement, and antibiotics.

    Epithelial Cell Medium Supplement Kit, Cat. No. H6621SF-Kit includes:

    - 0.5 ML EGF

    - 0.5 ML Hydrocortisone

    - 5.0 ML Antibiotic-Antimycotic Solution

    *Before use, add M6621SF Supplement Kit (M6621SF-Kit) into a 500ml basal medium.

    Storage Condition

    Store the basal medium at 2-8°C. Store epithelial cell growth supplement and antibiotics at -20°C. The complete cell culture medium with Supplement Kit can be kept at 4°C for two months. Protect from light.

    Note: To assure sterility after 2 weeks or if there is concern that sterility was compromised during the supplementation process, the prepared medium may be refiltered with a 0.2 um filter.

    Shipping

    Ice pack and dry ice.

    Authorized Uses of Cell Biologics Products

    Epithelial Cell Media from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ Cells or Products from the purchaser to other markets, organizations, or individuals is prohibited by Cell Biologics. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

    Culture protocol in four steps

    1. 1
      Thaw37 °C water bath, under 2 minutes. Never re-freeze a thawed vial.
    2. 2
      PlateSeed into a flask pre-coated with Cell Biologics coating solution, in the matched complete medium.
    3. 3
      FeedChange medium every 2–3 days until the culture is 80–90 % confluent.
    4. 4
      PassageSplit 1:2 with Cell Biologics trypsin; expand for 3–5 passages.

    Specification sheet

    Source

    Format & QC

    Format500 ML
    ShippingDry ice, overnight (US)

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