Rat Liver Kupffer Cells

Rat Primary Liver Kupffer Cells

Catalog No. RA-6226S (Live cells in a plate/wells only)

Suggested Medium

M3368 Complete Macrophage Medium /w Kit (500 ml)

Product Description

Rat Primary Liver Kupffer Cells from Cell Biologics are isolated from Sprague-Dawley rat liver tissues and grown in tissue culture plates with Cell Biologics’ Cell Culture Medium. Live Cells at passage 0 in culture plates/wells or flasks are shipped and cells are ready used for experiments. Cells are characterized by immunofluorescence staining using an antibody of CD11b. Primary Liver Kupffer Cells can not be cultured indefinitely and can not be passaged.

Handling of Arriving Live Cells

Live Cells at passage 0 in culture plates or flasks are shipped overnight. When you receive the live cells in plates or flasks, remove the sticker from the filter cap, and keep the plates or flasks with 3-15 ml existing medium in a 37°C CO₂ incubator for 1 hour before replacing the desired Cell Biologics' cell culture medium.

Authorized Uses of Cell Biologics’ Products

Rat Primary Liver Kupffer Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.

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Primary Liver Kupffer Cell Culture Protocol

All cell culture procedures must be conducted in a bio-safety cabinet.

Any and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.

Use aseptic technique to prevent microbial contamination.

Medium

Review the information provided on the Cell Biologics website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture media (30-50 ML, Catalog No. M3368) to seed cells and when changing media.

Handling of Arriving Live Cells

Live Cells at passage 0 in a culture plate are shipped overnight. When you receive the live cells in a plate or flask, remove the sticker from the filter cap, and keep the plate or flask with 6-15 ml of existing medium in a 37°C CO₂ incubator for 1 hour before replacing the desired Cell Biologics' cell culture medium.

Handling of Arriving Live Cells

Live Cells at passage 0 in a culture plate or flask are shipped overnight. When you receive the live cells in a plate or flask, keep the plate or flask with suggested medium volumn in 37°C CO² incubator for 1 hour before replacing the desired Cell Biologics' cell culture medium.

Note: A recommended media volume for culture dishes, plates and flasks:

6-well plate: Use 2 ml medium per well;

12-well plate: Use 0.5 ml medium per well;

96-well: Use 100 ul medium per well;

Cells in T25 flask; Use 5ml medium;

Cells in T75 flask: Use 10 ml medium

Proceed with experiment assays.