Human Primary Dermal Fibroblasts
- Human
- Dermal
- Fibroblast
- Shipped on dry ice overnight within the US, 2-day international
- CoA per lot on request
- Performance guarantee replaced free if cells fail to attach
For research use only. Not for use in diagnostic or therapeutic procedures.
- SpeciesHuman
- TissueDermal
- Cell typeFibroblast
- ModelNormalUnmodified
- FormatFrozen Vial(0.5 x 10⁶ cells)
- MediumM2267Required complete medium
Description
Human Primary Dermal Fibroblasts- Adult
Catalog No. H-6067
Suggested Medium
M2267 Complete Fibroblast Medium w/ Kit – 500 ml
Product Description
Human Primary Dermal Fibroblasts from Cell Biologics are isolated from human skin tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Dermal Fibroblasts are characterized by their spindle morphology and immunofluorescent staining with anti-fibroblast-specific protein 1 or anti-Vimentin antibody. These cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.
Storage
Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.
Authorized Uses of Cell Biologics’ Products
Human Primary Dermal Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
Disclaimer
Investigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
Warranty and Liability
Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.
Publication:
Nat Commun. 2021; 12: 3615.
Culture protocol in four steps
- 1Thaw37 °C water bath, under 2 minutes. Never re-freeze a thawed vial.
- 2PlateSeed into a flask pre-coated with Cell Biologics coating solution, in M2267 complete medium.
- 3FeedChange medium every 2–3 days until the culture is 80–90 % confluent.
- 4PassageSplit 1:2 with Cell Biologics trypsin; expand for 3–5 passages.
Specification sheet
Source
| Species | Human |
|---|---|
| Tissue | Dermal |
| Cell type | Fibroblast |
| Modification | Unmodified |
Format & QC
| Format | Frozen Vial (0.5 x 10⁶ cells) |
|---|---|
| Also as | T25 Flask, T75 Flask |
| Storage | Liquid nitrogen on arrival |
| Shipping | Dry ice, overnight (US) |
+
M2267
Citations
- P32-specific CAR T cells with dual antitumor and antiangiogenic therapeutic potential in gliomas
Liat Rousso-Noori,#1 Ignacio Mastandrea,#1 Shauli Talmor,1 Tova Waks,2,3 Anat Globerson Levin,2 Maarja Haugas,4 Tambet Teesalu,4,5,6 Luis Alvarez-Vallina,7,8,9 Zelig Eshhar,2,3,10 and Dinorah Friedmann-Morvinski1,11. Nat Commun. 2021; 12: 3615.
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