GFP-Expressing Rat Primary Artery Smooth Muscle Cells

GFP-Expressing Rat Primary Artery Smooth Muscle Cells

Catalog No. RA-6081GFP

Suggested Medium

M2268 Complete Smooth Muscle Cell Medium w/ Kit – 500 ml

Product Description

GFP-Expressing Rat Primary Colonic Smooth Muscle Cells from Cell Biologics are isolated from the colon tissues of 6-8 weeks old laboratory Sprague-Dawley rat and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10⁶ cells per ml and is delivered frozen. GFP-Expressing Rat Primary Colonic Smooth Muscle Cells are characterized by immunofluorescence staining with antibody of α-smooth muscle actin and are negative for bacteria, yeast, fungi, and mycoplasma. These cells can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.

The green fluorescent protein (GFP) gives rise to very bright green fluorescence when exposed to blue light. Cell Biologics generates various GFP-expressing stable cells. GFP-expressing cells are developed through transducing GFP-lentiviral particles.

Storage

Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Authorized Uses of Cell Biologics’ Products

GFP-Expressing Rat Primary Artery Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human or monkey use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Investigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.

Cell Culture Protocol

MSDS