DMEM/Ham's F-12 (1:1 Mixture), With L-glutamine and HEPES

DMEM/Ham's F-12 (1:1 Mixture), With L-glutamine and HEPES

Catalog No. M6217

Description

Since Harry Eagle's first reports, studies have been underway to determine the media requirements for individual cell types. The medium found to be most satisfactory for these studies as a 1:1 mixture of Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F-12 Nutrient Mixture. To help compensate for the buffering capacity lost with the removal of serum, HEPES has been added in some modifications at a final concentration of 15 mM.

D-glucose 3151mg/L

L-Glutamine 365mg/L

Sodium Pyruvate 55mg/L

Phenol Red 8.1mg/L

Storage Condition

Store the basal medium at 2-8°C. Store cell growth supplement, fetal bovine serum (FBS) and antibiotics at -20°C. The complete cell culture medium with Supplement Kit can be kept in 4°C for two months. Protect from light.

To assure sterility, the prepared medium should be filtered with a 0.2 um filter after 2 weeks or if there is concern that sterility was compromised during the supplementation process.

Shipping

Ice pack.

Authorized Uses of Cell Biologics Products

Cell Medium from Cell Biologics are distributed for internal in vitro research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics, without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.