Complete Neutrophil Medium /w Kit
- Shipped on dry ice overnight within the US, 2-day international
- CoA per lot on request
- Performance guarantee replaced free if cells fail to attach
For research use only. Not for use in diagnostic or therapeutic procedures.
- ModelNormal
- Format500 ML
Description
Complete Neutrophil Medium /w Kit – 500 ML
Catalog No: M3367
(500 ml Basal medium with M3367-Kit)
Description
Neutrophil Medium is a complete medium designed for the culture of neutrophils. It was tested and optimized with neutrophil culture in vitro. It is formulated for use with 5% CO2 and 95% air in a humidified incubator.
The medium consists of 500 ml of basal medium (containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals), supplemented with neutrophil culture supplement, antibiotics, and fetal bovine serum.
Neutrophil Medium Supplement Kit, Cat. No. M3367-Kit includes:
- 5.0 ML Penicillin-Streptomycin
- 25.0 ML FBS
*Before use, add M3367-Kit to 500ml basal medium.
Storage Condition
Store the basal medium at 2-8°C. Store cell growth supplement, fetal bovine serum (FBS) and antibiotics at -20°C. The complete cell culture medium with Supplement Kit can be kept in 4°C for two months. Protect from light.
To assure sterility, the prepared medium should be filtered with a 0.2 um filter after 2 weeks or if there is concern that sterility was compromised during the supplementation process.
Shipping
Ice pack and dry ice.
Authorized Uses of Cell Biologics Products
Neutrophil Media from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
Culture protocol in four steps
- 1Thaw37 °C water bath, under 2 minutes. Never re-freeze a thawed vial.
- 2PlateSeed into a flask pre-coated with Cell Biologics coating solution, in the matched complete medium.
- 3FeedChange medium every 2–3 days until the culture is 80–90 % confluent.
- 4PassageSplit 1:2 with Cell Biologics trypsin; expand for 3–5 passages.
Specification sheet
Source
Format & QC
| Format | 500 ML |
|---|---|
| Shipping | Dry ice, overnight (US) |
Need something custom?
Different species, strain, modification or format? Our scientists isolate and engineer cells to order.






