M1365In stock — ships in 48 hrsCell Culture Media

Complete Hepatocyte Medium /w Kit

    Format
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    • Shipped on dry ice overnight within the US, 2-day international
    • CoA per lot on request
    • Performance guarantee replaced free if cells fail to attach

    For research use only. Not for use in diagnostic or therapeutic procedures.

    $95.00
    • ModelNormal
    • Format125 ML

    Description

    Complete Hepatocyte Medium /w Kit – 125 ML

    Catalog No: M1365

    Description

    Hepatocyte Medium is a complete medium designed for the culture of Hepatocytes. It was tested and optimized with Hepatocyte growth in vitro. It is formulated for use with 5% CO2 and 95% air in a humidified incubator.

    The medium consists of 125 ml of basal medium (containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals), supplemented with cell growth supplement, antibiotics, and fetal bovine serum.

    Hepatocyte Medium includes:

    - EGF

    - Insulin

    - Hydrocortisone

    - Antibiotic-Antimycotic Solution

    - FBS

    Storage Condition

    Store the medium at -20°C until use. The complete cell culture medium can be kept in 4°C for two months. Protect from light.

    To assure sterility, the prepared medium should be filtered with a 0.2 um filter after 2 weeks or if there is concern that sterility was compromised during the supplementation process.

    Shipping

    Dry ice.

    Authorized Uses of Cell Biologics Products

    Hepatocyte Media from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics, without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

    Culture protocol in four steps

    1. 1
      Thaw37 °C water bath, under 2 minutes. Never re-freeze a thawed vial.
    2. 2
      PlateSeed into a flask pre-coated with Cell Biologics coating solution, in the matched complete medium.
    3. 3
      FeedChange medium every 2–3 days until the culture is 80–90 % confluent.
    4. 4
      PassageSplit 1:2 with Cell Biologics trypsin; expand for 3–5 passages.

    Specification sheet

    Source

    Format & QC

    Format125 ML
    ShippingDry ice, overnight (US)

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