M5569In stock — ships in 48 hrsCell Culture Media

Complete Hepatic Stellate Cell Medium

    Format
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    • Shipped on dry ice overnight within the US, 2-day international
    • CoA per lot on request
    • Performance guarantee replaced free if cells fail to attach

    For research use only. Not for use in diagnostic or therapeutic procedures.

    $118.00
    • ModelNormal
    • Format125 ML

    Description

    Complete Hepatic Stellate Cell Medium/with Kit –125 ML

    Catalog Number: M5569

    (125 ml Basal medium with the growth factor supplement)

    Description

    Complete Hepatic Stellate Cell Medium is a medium designed for the culture of human or animal cells. It was tested and optimized with hepatic stellate cell growth in vitro. It is formulated for use with 5% CO2 and 95% air in a humidified incubator. The medium consists of 500 ml of basal medium (containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals).

    Complete Hepatic Stellate Cell Medium includes:

    EGF

    FGF

    Hydrocortisone

    Penicillin-Streptomycin

    FBS

    Storage Condition

    Store complete Hepatic Stellate Cell Culture Medium at -20°C until use. The complete cell culture medium can be kept in 4°C for two months. Protect from light.

    Note: To assure sterility after 2 weeks or if there is concern that sterility was compromised during the supplementation process, the prepared medium may be refiltered with a 0.2 um filter.

    Shipping

    Ice pack and dry ice.

    Authorized Uses of Cell Biologics Products

    Complete Hepatic Stellate Cell Medium from Cell Biologics are distributed for inter research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ Cells or Products from the purchaser to other markets, organizations, or individuals is prohibited by Cell Biologics. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

    Culture protocol in four steps

    1. 1
      Thaw37 °C water bath, under 2 minutes. Never re-freeze a thawed vial.
    2. 2
      PlateSeed into a flask pre-coated with Cell Biologics coating solution, in the matched complete medium.
    3. 3
      FeedChange medium every 2–3 days until the culture is 80–90 % confluent.
    4. 4
      PassageSplit 1:2 with Cell Biologics trypsin; expand for 3–5 passages.

    Specification sheet

    Source

    Format & QC

    Format125 ML
    ShippingDry ice, overnight (US)

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