CD-1227In stock — ships in 48 hrsPrimary Cells

CD1 Mouse Primary Kidney Cells

  • Mouse
  • Kidney
Format
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  • Shipped on dry ice overnight within the US, 2-day international
  • CoA per lot on request
  • Performance guarantee replaced free if cells fail to attach

For research use only. Not for use in diagnostic or therapeutic procedures.

$410.00
  • SpeciesMouseCD1
  • TissueKidney
  • ModelNormalUnmodified
  • FormatFrozen Vial(1 x 10⁶ cells)
  • MediumM5567Required complete medium

Description

CD1 Mouse Primary Kidney Cells

Catalog No. CD-1227

Suggested Medium: Cell Culture Medium /w Kit – 500 ml

Catalog No. M5567

Product Description

CD1 Mouse Primary Kidney Cells from Cell Biologics are isolated from the kidney tissue of pathogen-free laboratory mice. CD1 Mouse Primary Kidney Cells are mixed population of CD1 Mouse kidney cells grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x106 cells per ml and is delivered frozen.

Product Testing

CD1 Mouse Primary Kidney Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Laboratory Applications

CD1 Mouse Primary Kidney Cells can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, or immunofluorescent flow cytometry.

Storage of Cell Biologics Products

Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request.

Never can primary cells be kept at -20 °C.

Authorized Uses of Cell Biologics Products

CD1 Mouse Primary Kidney Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Although CD1 Mouse Primary Kidney Cells are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from Cell Biologicswith caution, since no test procedure can completely guarantee the absence of infectious agents.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.

Culture protocol in four steps

  1. 1
    Thaw37 °C water bath, under 2 minutes. Never re-freeze a thawed vial.
  2. 2
    PlateSeed into a flask pre-coated with Cell Biologics coating solution, in M5567 complete medium.
  3. 3
    FeedChange medium every 2–3 days until the culture is 80–90 % confluent.
  4. 4
    PassageSplit 1:2 with Cell Biologics trypsin; expand for 3–5 passages.
Full protocol (PDF) →

Specification sheet

Source

SpeciesMouse
StrainCD1
TissueKidney
ModificationUnmodified

Format & QC

FormatFrozen Vial (1 x 10⁶ cells)
Also asT25 Flask, T75 Flask
StorageLiquid nitrogen on arrival
ShippingDry ice, overnight (US)

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