CD-1 Mouse Bone Marrow Mesenchymal Stem Cells
- Mouse
- Bone Marrow
- Stem Cell
- Shipped on dry ice overnight within the US, 2-day international
- CoA per lot on request
- Performance guarantee replaced free if cells fail to attach
For research use only. Not for use in diagnostic or therapeutic procedures.
- SpeciesMouse
- TissueBone Marrow
- Cell typeStem Cell
- ModelNormal
- FormatFrozen Vial(0.5 x 10⁶ cells)
- MediumM5556Required complete medium
Description
CD1 Mouse Bone Marrow Mesenchymal Stem Cells
Catalog No. CD-1043
Suggested Medium
M5556 Mesenchymal Cell Medium w/ Kit (500 ml)
Product Description
CD1 Mouse Bone Marrow Mesenchymal Stem Cells from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory CD1 mice and grown in tissue culture flasks with Cell Biologics’ Complete Growth Medium. Cells at passage 1-2 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml. CD1 Mouse Bone Marrow Mesenchymal Stem Cells are characterized by immunofluorescence staining with antibody of CD44, Sca-1 or CD29. These cells can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.
Storage
Cryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.
Authorized Uses of Cell Biologics’ Products
CD1 Mouse Bone Marrow Mesenchymal Stem Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
Disclaimer
Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Warranty and Liability
Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.
Culture protocol in four steps
- 1Thaw37 °C water bath, under 2 minutes. Never re-freeze a thawed vial.
- 2PlateSeed into a flask pre-coated with Cell Biologics coating solution, in M5556 complete medium.
- 3FeedChange medium every 2–3 days until the culture is 80–90 % confluent.
- 4PassageSplit 1:2 with Cell Biologics trypsin; expand for 3–5 passages.
Specification sheet
Source
| Species | Mouse |
|---|---|
| Tissue | Bone Marrow |
| Cell type | Stem Cell |
Format & QC
| Format | Frozen Vial (0.5 x 10⁶ cells) |
|---|---|
| Also as | T25 Flask, T75 Flask |
| Storage | Liquid nitrogen on arrival |
| Shipping | Dry ice, overnight (US) |
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M5556
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