C57BL/6-GFP Mouse Primary Tracheal Fibroblasts

C57BL/6-GFP Mouse Primary Tracheal and Bronchial Fibroblasts

Catalog No. mGFP-6217

Suggested Medium: Fibroblast Medium /w Kit - 500 ml

Catalog No. M2267

Product Description

C57BL/6-GFP Mouse Primary Tracheal and Bronchial Fibroblasts from Cell Biologicsare isolated from C57BL/6-Tg(CAG-EGFP)1Osb/J mouse tracheal and bronchial tissue of pathogen-free laboratory mice. C57BL/6-GFP Mouse Primary Tracheal and Bronchial Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell BiologicsCulture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10⁶ cells per ml and is delivered frozen.

Product Testing

C57BL/6-GFP Mouse Primary Tracheal and Bronchial Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 (Millipore USA) by immunofluorescence staining. C57BL/6-GFP Mouse Primary Tracheal and Bronchial Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Laboratory Applications

C57BL/6-GFP Mouse Primary Tracheal and Bronchial Fibroblasts can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Storage of Cell Biologics Products

Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request. Never can primary cells be kept at -20 °C.

Authorized Uses of Cell Biologics Products

C57BL/6-GFP Mouse Primary Tracheal and Bronchial Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Although C57BL/6-GFP Mouse Tracheal and Bronchial Fibroblasts are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.