
Representative image (Cell Biologics)
B129 Mouse Peritoneal Macrophages - Thioglycollate-elicited (Frozen Cells)
- Mouse
- Macrophage
- Shipped on dry ice overnight within the US, 2-day international
- CoA per lot on request
- Performance guarantee replaced free if cells fail to attach
For research use only. Not for use in diagnostic or therapeutic procedures.
- SpeciesMouseB129
- Cell typeMacrophage
- ModelNormalUnmodified
- FormatFrozen Vial(3 x 10⁶ cells)
- MediumM3368Required complete medium
Description
B129 Mouse Peritoneal Macrophages - Thioglycollate-elicited (Frozen Cells)
Catalog No. B129-7032TF
Suggested Medium: Macrophage Medium /w Kit (500 ml)
Catalog No. M3368
Product Description
B129 Mouse Peritoneal Macrophages (Thioglycollate-elicited) are isolated from the peritoneal cavity of pathogen-free laboratory adult mice. Cells at passage 0 are cryo-preserved in vials. Each vial contains 6x106 cells and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma and tested for expression of markers using antibody, CD11b by flow cytometry. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Cell Biologics. Primary Peritoneal Macrophages can not be cultured indefinitely and can not be passaged.
Laboratory Applications
B129 Mouse Peritoneal Macrophages (Thioglycollate-elicited) can be used in standard biochemical procedures include PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications.
Storage of Cell Biologics Products
Cell Biologics will ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use. Live-cell shipment is also available on request. Primary cells can never be kept at -20 °C.
Authorized Uses of Cell Biologics Products
B129 Mouse Peritoneal Macrophages (Thioglycollate-elicited) from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics, without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
Disclaimer
Investigators should handle the cells that they receive from Cell Biologics with caution and treat all Cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Warranty and Liability
Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.
Culture protocol in four steps
- 1Thaw37 °C water bath, under 2 minutes. Never re-freeze a thawed vial.
- 2PlateSeed into a flask pre-coated with Cell Biologics coating solution, in M3368 complete medium.
- 3FeedChange medium every 2–3 days until the culture is 80–90 % confluent.
- 4PassageSplit 1:2 with Cell Biologics trypsin; expand for 3–5 passages.
Specification sheet
Source
| Species | Mouse |
|---|---|
| Strain | B129 |
| Cell type | Macrophage |
| Modification | Unmodified |
Format & QC
| Format | Frozen Vial (3 x 10⁶ cells) |
|---|---|
| Also as | Cells in suspension 6 x 10^6 |
| Storage | Liquid nitrogen on arrival |
| Shipping | Dry ice, overnight (US) |
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M3368
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