CB6920In stock — ships in 48 hrsReagents & Supplies

Antibiotic/Antimycotic Solution (1:100)

    Format
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    • Shipped on dry ice overnight within the US, 2-day international
    • CoA per lot on request
    • Performance guarantee replaced free if cells fail to attach

    For research use only. Not for use in diagnostic or therapeutic procedures.

    $50.00
    • ModelNormal
    • Format100 ml

    Description

    Antibiotic/Antimycoric Solution (1:100)

    Catalog Number: CB6920

    Description

    Antibiotic-Antimycotic is used to prevent bacterial and fungal contamination. The combination of the antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective action against gram-positive and gram-negative bacteria, respectively. Amphotericin B is used to prevent fungal contamination of cell cultures due to its inhibition of multicellular fungus and yeast.

    Penicillin was originally purified from the fungus Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus. Streptomycin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Amphotericin B is an antifungal agent that prevents the growth of fungi and yeast by causing an increase in fungal plasma membrane permeability.

    Formulation

    Contains 10,000 units of penicillin (base), 10,000 ug of streptomycin (base), and 25 ug of amphotericin B/ml utilizing penicillin G (sodium salt), streptomycin sulfate, and amphotericin B as Antimycotic in 0.85% saline.

    Storage Condition

    Store the solution at -20°C.

    Shipping

    Dry Ice.

    Authorized Uses of Cell Biologics Products

    Antibiotic-Antimycotic Solution from Cell Biologics are distributed for internal in vitro research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics, without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

    Culture protocol in four steps

    1. 1
      Thaw37 °C water bath, under 2 minutes. Never re-freeze a thawed vial.
    2. 2
      PlateSeed into a flask pre-coated with Cell Biologics coating solution, in the matched complete medium.
    3. 3
      FeedChange medium every 2–3 days until the culture is 80–90 % confluent.
    4. 4
      PassageSplit 1:2 with Cell Biologics trypsin; expand for 3–5 passages.

    Specification sheet

    Source

    Format & QC

    Format100 ml
    ShippingDry ice, overnight (US)

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