Aged Rat Skeletal Muscle Cells
- Rat
- Skeletal
- Skeletal Muscle Cell
- Aged
- Shipped on dry ice overnight within the US, 2-day international
- CoA per lot on request
- Performance guarantee replaced free if cells fail to attach
For research use only. Not for use in diagnostic or therapeutic procedures.
- SpeciesRat
- TissueSkeletal
- Cell typeSkeletal Muscle Cell
- ModelAgedUnmodified
- FormatFrozen Vial(0.5 x 10⁶ cells)
- MediumM1261Required complete medium
Description
Aged Rat Primary Skeletal Muscle Cells
Catalog No. AR-6167
Suggested Medium
Skeletal Muscle Cell Medium /w Kit – 500 ML
Catalog No: M1261
(500 ml Basal medium with M1261-Kit)
Product Description
Aged Rat Primary Skeletal Muscle Cells from Cell Biologics are isolated from the skeletal muscle tissues of 62-70 week old rats and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Aged Rat Primary Skeletal Muscle Cells are characterized by immunofluorescence staining with antibodies of myosin and/or actinin and are negative for bacteria, yeast, fungi, and mycoplasma. These cells can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.
Storage
Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.
Authorized Uses of Cell Biologics’ Products
Aged Rat Primary Skeletal Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
Disclaimer
Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Warranty and Liability
Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.
Cell Culture Protocol
https://cellbiologics.com/document/1708462493.pdf
Culture protocol in four steps
- 1Thaw37 °C water bath, under 2 minutes. Never re-freeze a thawed vial.
- 2PlateSeed into a flask pre-coated with Cell Biologics coating solution, in M1261 complete medium.
- 3FeedChange medium every 2–3 days until the culture is 80–90 % confluent.
- 4PassageSplit 1:2 with Cell Biologics trypsin; expand for 3–5 passages.
Specification sheet
Source
| Species | Rat |
|---|---|
| Tissue | Skeletal |
| Cell type | Skeletal Muscle Cell |
| Model | Aged |
| Modification | Unmodified |
Format & QC
| Format | Frozen Vial (0.5 x 10⁶ cells) |
|---|---|
| Also as | T25 Flask, T75 Flask |
| Storage | Liquid nitrogen on arrival |
| Shipping | Dry ice, overnight (US) |
+
M1261
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