{"product_id":"rat-primary-lung-microvascular-endothelial-cells-rn-6011","title":"Rat Primary Lung Microvascular Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eRat Primary \u003c\/b\u003e\u003cb\u003eLung Microvascular \u003c\/b\u003e\u003cb\u003eEndothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eRN-6011\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Endothelial Cell Medium \/w Kit – 500 ml\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eM1266\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eProduct Description\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eRat Primary Lung Microvascular Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from lung tissue of 1-day-old neonatal laboratory Sprague-Dawley rats. Rat Primary Lung Microvascular Endothelial Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics\u003c\/i\u003e’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. \u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eProduct Testing\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eRat Primary Lung Microvascular Endothelial Cells are tested for expression of markers using antibody, PECAM-1 Antibody (M-20, sc-1506, Santa Cruz) or ZO-1 Rabbit Polyclonal Antibody (Catalog No. 617300, Life Technologies) by immunofluorescence staining. Rat Primary Lung Microvascular Endothelial Cells are also tested for uptake of Dil-Ac-LDL (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. Rat Primary Lung Microvascular Endothelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eLaboratory Applications\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eRat Primary Lung Microvascular Endothelial Cells can be used in assays of cell-cell adhesion, migration, vascular tube formation, transendothelial resistance (TER). Standard biochemical procedures performed with cell cultures include RT-PCR, western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eStorage of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.\u003c\/p\u003e\u003cp\u003eNever can primary cells be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eRat Primary Lung Microvascular Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eDisclaimer\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eWarranty and Liability\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003ePublication:\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/entrez\/eutils\/elink.fcgi?dbfrom=pubmed\u0026amp;retmode=ref\u0026amp;cmd=prlinks\u0026amp;id=33607217\" target=\"_blank\" rel=\"noopener\"\u003eFree Radic Biol Med. 2021 Apr; 166: 73–89.\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC9238549\/\" target=\"_blank\" rel=\"noopener\"\u003eJ Am Heart Assoc.\u003c\/a\u003e 2022 May 17; 11(10): e025181.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527908512028,"sku":"RN-6011","price":560.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687902695708,"sku":"RN-6011","price":610.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687902728476,"sku":"RN-6011","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-fdc54d5946.jpg?v=1791767016","url":"https:\/\/jkpgye-36.myshopify.com\/products\/rat-primary-lung-microvascular-endothelial-cells-rn-6011","provider":"Cell Biologics","version":"1.0","type":"link"}