{"product_id":"rat-primary-hepatic-stellate-cells","title":"Rat Primary Hepatic Stellate Cells","description":"\u003cp\u003e\u003cstrong\u003eRat Primary Hepatic Stellate Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. RA-6242\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM5569 Complete Hepatic Stellate Cell Medium \/w Kit (125 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRat Primary Hepatic Stellate Cells from Cell Biologics are isolated from Sprague-Dawley rat liver tissues and grown in tissue culture plates with Cell Biologics’ Cell Culture Medium. Vitamin A droplets in Hepatic Stellate Cells are visualized by blue autofluorescence when excited with UV light. These cells can be used for experiments under the cell culture conditions specified by Cell Biologics. Hepatic Stellate Cells are not recommended for long-term cultures since these cells would differentiate into fibroblat-like cells after plating, and cells will not proliferate in culture and can not be passaged.\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRat Primary Hepatic Stellate Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e_____________________________________________________________________________________\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Hepatic Stellate Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet (BSL-2)\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eReview the information provided on the Cell Biologics’ website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture medium of M5569 (30-50 ML) to recover cryo-preserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCoating of Cell Culture Plates or Dishes\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCoat sterile culture dishes or flasks with Gelatin Coating Solution (Catalog No. 6950, Cell Biologics) for 2 min, then aspirate the excess solution.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell Recovery from Cryovial\u003c\/strong\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003e Thaw the cells quickly (\u0026lt;2 min) in a 37°C water bath until just prior to complete thawing.\u003c\/li\u003e\n\u003cli\u003e Wipe the outside of the vial with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Gently resuspend the cells with 5-7 ml pre-warmed Cell Biologics’ Cell Culture Medium (Catalog number M5569, Cell Biologics) and slowly pipette up and down 2 to 3 times to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003e Carefully pour cell suspension into 60 mm dish (or one well of 6-well plate).\u003c\/li\u003e\n\u003cli\u003e Once the cells have attached to the culture dishes about 80-90% (after 16-24h), gently aspirate supernatant (remove cell debris and non-adherent cells) and wash attached cells with the pre-warmed Maintenance Medium (Catalog number M5569, Cell Biologics) 2-3 time.\u003c\/li\u003e\n\u003cli\u003e Cells should be checked daily under a microscope to verify appropriate cell morphology.\u003c\/li\u003e\n\u003cli\u003e Proceed with experiment assays within 1-4 days after plating cells.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cp\u003e\u003cstrong\u003e\u003cu\u003eHandling of Arriving Live Cells\u003c\/u\u003e\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLive Cells at passage 0 in a culture plate or flask are shipped overnight. When you receive the live cells in a plate or flask, keep the plate or flask with suggested medium volumn in 37°C CO\u003csup\u003e2\u003c\/sup\u003e incubator for 1 hour before replacing the desired Cell Biologics' cell culture medium.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eNote: A recommended media volume for culture dishes, plates and flasks:\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e1 well of 6-well plate: Use 2 ml medium per well;\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e12-well plate: Use 0.5 ml medium per well;\u003cbr\u003e\u003c\/p\u003e\u003cp\u003eProceed with experiment assays.\u003c\/p\u003e\u003cp\u003ePlease send us the cell images (\u0026gt;80% confluence) if you have any question or problem with cultured cells.\u003c\/p\u003e\u003cp\u003ePublication:\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8722578\/\" target=\"_blank\" rel=\"noopener\"\u003eInt J Nanomedicine.\u003c\/a\u003e 2021; 16: 8375–8389.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.2 x 10^6 cells)","offer_id":54527919227164,"sku":"RA-6242","price":812.5,"currency_code":"USD","in_stock":true},{"title":"2 wells of 12-well plate","offer_id":54687906988316,"sku":"RA-6242","price":1075.0,"currency_code":"USD","in_stock":true},{"title":"2 wells of 24-well plate","offer_id":54687907021084,"sku":"RA-6242","price":950.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-34b0a0790d.jpg?v=1791766978","url":"https:\/\/jkpgye-36.myshopify.com\/products\/rat-primary-hepatic-stellate-cells","provider":"Cell Biologics","version":"1.0","type":"link"}