{"product_id":"rat-bone-marrow-dendritic-cells","title":"Rat Bone Marrow Dendritic Cells","description":"\u003cp\u003e\u003cstrong\u003eRat Bone Marrow Dendritic Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. RA-6200\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM7711 Complete Dendritic Cell Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRat Bone Marrow Dendritic Cells from Cell Biologics are isolated from Sprague-Dawley rat tibias and femurs. Cells at passage 0 are cryo-preserved and each vial contains1x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. Rat Bone Marrow Dendritic Cells are characterized by immunofluorescence staining with antibody of CD11c. These cells will not proliferate in culture and cannot be passaged.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRat Bone Marrow Dendritic Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e_____________________________________________________________________________________\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium:\u003c\/strong\u003e Use pre-warmed (37°C) cell culture media of Catalog No. M7711 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell recovery from cryovial:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (2 x 10^6 cells)","offer_id":54527895109916,"sku":"RA-6200","price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-31bc77c288.jpg?v=1791767016","url":"https:\/\/jkpgye-36.myshopify.com\/products\/rat-bone-marrow-dendritic-cells","provider":"Cell Biologics","version":"1.0","type":"link"}