{"product_id":"mouse-tumor-associated-fibroblasts-mouse-lewis-lung-cancer-origin","title":"Mouse Tumor-Associated Fibroblasts (Mouse Lewis Lung Cancer Origin)","description":"\u003cp\u003e\u003cb\u003eMouse Tumor-Associated Fibroblasts (Mouse Lewis Lung Cancer Origin)\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eMC-6013m\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Fibroblast Medium \/w Kit - 500 ml\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eM2267\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eMouse Tumor-Associated Fibroblasts (Mouse Lewis Lung Cancer Origin, MTLUFb) from \u003ci\u003eCell Biologics\u003c\/i\u003e were isolated from human xenograft of pathogen-free laboratory nude mice. Mouse Lewis lung adenocarcinoma epithelial cells were subcutaneously injected into the nude mice for 4-6 weeks and the tumor tissues were then harvested for cell culture. MTLUFb were cultured in a T75 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 14-35 days. Cells were detached from flasks and immediately cryo-preserved in vials. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Testing\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eMTLUFb are characterized by immunofluorescent staining with antibody of anti-FSP1\/S100A4 (Millipore USA) by immunofluorescence staining. Cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eLaboratory Applications \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eMTLUFb can be used in assays of standard biochemical procedures performed with cell cultures include cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/b\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of \u003c\/strong\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e Products\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003eMTLUFb from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics,\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at\u003ca href=\"http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm\" target=\"_blank\" rel=\"noopener\"\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm\u003c\/a\u003e.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution, for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527718883612,"sku":"MC-6013m","price":937.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687863603484,"sku":"MC-6013m","price":1000.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687863636252,"sku":"MC-6013m","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-fdeff8eb79.jpg?v=1791766977","url":"https:\/\/jkpgye-36.myshopify.com\/products\/mouse-tumor-associated-fibroblasts-mouse-lewis-lung-cancer-origin","provider":"Cell Biologics","version":"1.0","type":"link"}