{"product_id":"human-hypertension-lung-microvascular-endothelial-cells","title":"Human Hypertension Lung Microvascular Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eHuman Hypertension Lung Microvascular Endothelial Cells (HPPUEC)\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eHP-6011\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Complete Human Endothelial Cell Medium– 500 ML\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eH1168\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eProduct Description\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Hypertension Lung Microvascular Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from the lung of human donor that has been diagnosed with hypertension. Human Hypertension Lung Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, Cells at passage 3 are detached from flasks and immediately cryopreserved in vails. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eProduct Testing\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Hypertension Lung Microvascular Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e display typical cobblestone with large dark nuclei appearance under light microscopy. Cells are tested for expression of endothelial cell marker using antibody, CD31 (Catalog No. 550389, BD; CD31\/PECAM-1 PE-conjugated Antibody, Catalog No. FAB3567P, R\u0026amp;D) or VE-Cadherin (FITC-VE-cadherin Catalog No. 560411, BD) by immunofluorescence staining or FACS. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and\/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased. Cells can be expanded for 3-5 passages under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eLaboratory Applications\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Hypertension Lung Microvascular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Hypertension Lung Microvascular Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eDisclaimer\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003ca href=\"http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm\" target=\"_blank\" rel=\"noopener\"\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eWarranty and Liability\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003cem\u003eCell Biologics’\u003c\/em\u003e human endothelial cells with \u003cem\u003eCell Biologics’\u003c\/em\u003e Media (catalog number H1168)and Coating Solution for appropriate endothelial cell culture and cell testing following \u003cem\u003eCell Biologics’\u003c\/em\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527708299548,"sku":"HP-6011","price":887.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687850529052,"sku":"HP-6011","price":950.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687850561820,"sku":"HP-6011","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-a939a886c7.jpg?v=1791767016","url":"https:\/\/jkpgye-36.myshopify.com\/products\/human-hypertension-lung-microvascular-endothelial-cells","provider":"Cell Biologics","version":"1.0","type":"link"}