{"product_id":"diabetic-mouse-dermal-epithelial-cells","title":"Diabetic Mouse Dermal Epithelial Cells","description":"\u003cp\u003e\u003cb\u003eDiabetic Mouse \u003c\/b\u003e\u003cb\u003eDermal Epithelial Cells \u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eMD-6066\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=m6621\"\u003eEpithelial Cell Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m6621\"\u003e\u003cb\u003eM6621\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eProduct Description\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDiabetic Mouse Dermal Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from the skin of Diabetic (db\/db) mice. Diabetic Mouse Dermal Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDiabetic Mouse Dermal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology). Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDiabetic Mouse Dermal Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDiabetic Mouse Dermal Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics \u003c\/i\u003ewith caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527722422556,"sku":"MD-6066","price":762.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687867830556,"sku":"MD-6066","price":825.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687867863324,"sku":"MD-6066","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-7a326d8e2e.jpg?v=1791767016","url":"https:\/\/jkpgye-36.myshopify.com\/products\/diabetic-mouse-dermal-epithelial-cells","provider":"Cell Biologics","version":"1.0","type":"link"}