{"product_id":"cre-expressing-rat-primary-bone-marrow-derived-endothelial-cells","title":"Cre-Expressing Rat Primary Bone Marrow-Derived Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eCre-Expressing Rat Primary Bone Marrow-Derived Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. RA-6221Cre\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1266 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCre-Expressing Rat Primary Bone Marrow-Derived Endothelial Cells from Cell Biologics are isolated from the bone marrow of Sprague-Dawley rats and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Cre-Expressing Rat Primary Bone Marrow-Derived Endothelial Cells are characterized by use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split Cre-Expressing Ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eCell Biologics generates the Cre-expressing stable cells by transducing Cre recombinase. LOXP constructs are transfected into the stable cells that can be performed various biological analyses.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCre-Expressing Rat Primary Bone Marrow-Derived Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527900090652,"sku":"RA-6221Cre","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-cb4421d9d0.jpg?v=1791767054","url":"https:\/\/jkpgye-36.myshopify.com\/products\/cre-expressing-rat-primary-bone-marrow-derived-endothelial-cells","provider":"Cell Biologics","version":"1.0","type":"link"}