{"product_id":"canine-bone-marrow-macrophages-frozen-cells","title":"Canine Bone Marrow Macrophages (Frozen Cells)","description":"\u003cp\u003e\u003cb\u003eCanine Bone Marrow Macrophages (Frozen Cells)\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eD-6030\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Macrophage Medium \/w Kit (500 ml) \u003c\/p\u003e\u003cp\u003e\u003cb\u003eCatalog No. M6030\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCanine Bone Marrow Macrophages are derived from the tibias or femurs of Beagle dog. Cells at passage 0 are cryo-preserved in vials. Each vial contains 3x10\u003csup\u003e6\u003c\/sup\u003e cells and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma and tested for expression of markers using antibody, CD11b by flow cytometry. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003ePrimary Bone Marrow Macrophages can not be cultured indefinitely and can not be passaged. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCanine Bone Marrow Macrophages can be used in standard biochemical procedures include PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e will ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use. Live-cell shipment is also available on request. Primary cells can never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCanine Bone Marrow Macrophages from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e, without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all Cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Macrophage Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a bio-safety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eMedium Review the information provided on the Cell Biologics website about appropriate culture media (e.g. serum and other supplements).\u003c\/p\u003e\u003cp\u003eUse pre-warmed (37°C) cell culture media (30-50 ML, Catalog No. M3368) to seed cells and when changing media.\u003c\/p\u003e\u003cp\u003eCoating of Cell Culture Plates or Dishes Coat sterile culture dishes or flasks with Gelatin Coating Solution (Catalog No. 6950, Cell Biologics) for 2 min, then aspirate the excess solution.\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell Recovery from Cryovial\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eThaw the cells quickly (\u0026lt;2 min) in a 37°C water bath until just prior to complete thawing.\u003c\/p\u003e\u003cp\u003eWipe the outside of the vial with 70% ethanol.\u003c\/p\u003e\u003cp\u003eUse 1 ml Pipette Tip to resuspend cells and transfer cells to a 15 ml sterile tube.\u003c\/p\u003e\u003cp\u003eAdd 5 ml of pre-warmed Cell Biologics’ Cell Culture Medium (Catalog number M3368, Cell Biologics) gently to the side of the tube and slowly pipette up and down 2 to 3 times to resuspend the cells.\u003c\/p\u003e\u003cp\u003eCentrifuge the cells at 200 x g at 4°C for 5 min.\u003c\/p\u003e\u003cp\u003eCarefully remove the supernatant and resuspend in 7 mL pre-warmed Maintenance Medium (Catalog No. M3368, Cell Biologics).\u003c\/p\u003e\u003cp\u003eCarefully pour cell suspension into the cell culture plate or dish.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRecommended Cell Seeding: \u003c\/strong\u003e0.7-0.8 million cells are seeded per well of a 12-well plate or 1-1.5 million macroPlace a plate in a humidified, 5%-CO\u003csub\u003e2\u003c\/sub\u003e incubator at 37°C until experiments.\u003c\/p\u003e\u003cp\u003eChange fresh cell culture medium every 24-48 hours. Cells should be checked daily under a microscope to verify appropriate cell morphology.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProceed with experiment assays.\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eNote: Please send us the cell images (\u0026gt;90% confluence) if you have any question or problem with cultured cells.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (3 x 10^6 cells)","offer_id":54527587221788,"sku":"D-6030","price":680.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-5c584d3d0f.jpg?v=1791766977","url":"https:\/\/jkpgye-36.myshopify.com\/products\/canine-bone-marrow-macrophages-frozen-cells","provider":"Cell Biologics","version":"1.0","type":"link"}