{"product_id":"c57bl-6-gfp-mouse-primary-liver-fibroblasts","title":"C57BL\/6-GFP Mouse Primary Liver Fibroblasts","description":"\u003cp\u003e\u003cb\u003eC57BL\/6-GFP Mouse Primary Liver Fibroblasts\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003emGFP-6019\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=M2267\"\u003eFibroblast Medium \/w Kit - 500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=M2267\"\u003e\u003cb\u003eM2267\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e\u003cu\u003eProduct Description\u003c\/u\u003e\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6-GFP Mouse Primary Liver Fibroblasts from \u003cem\u003eCell Biologics\u003c\/em\u003eare isolated from C57BL\/6-Tg(CAG-EGFP)1Osb\/J mouse liver tissue of pathogen-free laboratory mice. C57BL\/6-GFP Mouse Primary Liver Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003cem\u003eCell Biologics\u003c\/em\u003eCulture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. \u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e\u003cu\u003eProduct Testing\u003c\/u\u003e\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6-GFP Mouse Primary Liver Fibroblasts are tested for expression of marker using the antibody of anti-FSP1\/S100A4 (Millipore USA) by immunofluorescence staining. C57BL\/6-GFP Mouse Primary Liver Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e\u003cu\u003eLaboratory Applications \u003c\/u\u003e\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6-GFP Mouse Primary Liver Fibroblasts can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eStorage of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e\u003c\/b\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request. Never can primary cells be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e\u003cu\u003eAuthorized Uses of \u003c\/u\u003e\u003c\/strong\u003e\u003cem\u003e\u003cb\u003e\u003cu\u003eCell Biologics\u003c\/u\u003e\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e\u003cu\u003e Products\u003c\/u\u003e\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6-GFP Mouse Primary Liver Fibroblasts from \u003cem\u003eCell Biologics\u003c\/em\u003e are distributed for research purposes only. Our products are not authorized for human use, for \u003cem\u003ein vitro \u003c\/em\u003ediagnostic procedures, or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics \u003c\/i\u003ewithout the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eDisclaimer\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAlthough C57BL\/6-GFP Mouse Liver Fibroblasts are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eWarranty and Liability\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527724650780,"sku":"mGFP-6019","price":737.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687870910748,"sku":"mGFP-6019","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687870943516,"sku":"mGFP-6019","price":937.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-16c0aba89f.jpg?v=1791767054","url":"https:\/\/jkpgye-36.myshopify.com\/products\/c57bl-6-gfp-mouse-primary-liver-fibroblasts","provider":"Cell Biologics","version":"1.0","type":"link"}