{"title":"Rat Macrophages","description":"\u003cp\u003eRat Macrophages from Cell Biologics, isolated and quality-tested in our ISO 9001 laboratory for research use.\u003c\/p\u003e","products":[{"product_id":"rat-bone-marrow-macrophages-frozen-cells","title":"Rat Bone Marrow Macrophages (Frozen Cells)","description":"\u003cp\u003e\u003cstrong\u003eRat Bone Marrow Macrophages\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. RA-6030F\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM3368 Complete Macrophage Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRat Bone Marrow Macrophages from Cell Biologics are isolated from Sprague-Dawley rat tibias and femurs and grown in tissue culture plates with Cell Biologics’ Cell Culture Medium. Cells at passage 0 are cryo-preserved and each vial contains 3 x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. Rat Bone Marrow Macrophages are characterized by immunofluorescence staining using antibody of CD11b or F4\/80. These cells can be plated on multiwell culture plates for experiments under the cell culture conditions specified by Cell Biologics. Primary Bone Marrow Macrophages can not be cultured indefinitely and can not be passaged. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRat Bone Marrow Macrophages from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e________________________________________________________________________________________\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Macrophage Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a bio-safety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eReview the information provided on the Cell Biologics website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture media (30-50 ML, Catalog No. M3368) to seed cells and when changing media.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCoating of Cell Culture Plates or Dishes\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCoat sterile culture dishes or flasks with Gelatin Coating Solution (Catalog No. 6950, Cell Biologics) for 2 min, then aspirate the excess solution.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell Recovery from Cryovial\u003c\/strong\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003e Thaw the cells quickly (\u0026lt;2 min) in a 37°C water bath until just prior to complete thawing.\u003c\/li\u003e\n\u003cli\u003e Wipe the outside of the vial with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Use 1 ml Pipette Tip to resuspend cells and transfer cells to a 15 ml sterile tube.\u003c\/li\u003e\n\u003cli\u003e Add 5 ml of pre-warmed Cell Biologics’ Cell Culture Medium (Catalog number M3368, Cell Biologics) gently to the side of the tube and slowly pipette up and down 2 to 3 times to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003e Centrifuge the cells at 200 x g at 4°C for 5 min.\u003c\/li\u003e\n\u003cli\u003e Carefully remove the supernatant and resuspend in 7 mL pre-warmed Maintenance Medium (Catalog No. M3368, Cell Biologics).\u003c\/li\u003e\n\u003cli\u003e Carefully pour cell suspension into the cell culture plate or dish.\u003c\/li\u003e\n\u003cli\u003e Recommended Cell Seeding: 0.7-0.8 million cells are seeded per well of a 12-well plate or 1-1.5 million macroPlace a plate in a humidified, 5%-CO\u003csub\u003e2\u003c\/sub\u003e incubator at 37°C until experiments.\u003c\/li\u003e\n\u003cli\u003e Change fresh cell culture medium every 24-48 hours.\u003c\/li\u003e\n\u003cli\u003e Cells should be checked daily under a microscope to verify appropriate cell morphology.\u003c\/li\u003e\n\u003cli\u003e Proceed with experiment assays.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cp\u003eNote: Please send us the cell images (\u0026gt;90% confluence) if you have any question or problem with\u003c\/p\u003e\u003cp\u003ecultured cells.\u003c\/p\u003e\u003cp\u003ePublication:\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/entrez\/eutils\/elink.fcgi?dbfrom=pubmed\u0026amp;retmode=ref\u0026amp;cmd=prlinks\u0026amp;id=26443597\" target=\"_blank\" rel=\"noopener\"\u003eCirc Res. 2015 Nov 6; 117(11): e80–e89.\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (3 x 10^6 cells)","offer_id":54527871844636,"sku":"RA-6030","price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-76289ca95f.jpg?v=1791767087"}],"url":"https:\/\/jkpgye-36.myshopify.com\/collections\/rat-macrophages.oembed","provider":"Cell Biologics","version":"1.0","type":"link"}