{"title":"Rat Hepatocytes, Stellate Cells, Kupffer Cells","description":"\u003cp\u003ePrimary Hepatocytes should be used for experiments within a week after received.\u003c\/p\u003e","products":[{"product_id":"rat-primary-hepatocytes-plateable","title":"Rat Primary Hepatocytes - Plateable","description":"\u003cp\u003e\u003cstrong\u003e_______________________________________________________________________________________\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRat Primary Hepatocytes - Plateable\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. RA-6224F\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1265 Complete Hepatocyte Medium \/w Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRat Primary Hepatocytes from Cell Biologics are isolated from Sprague-Dawley rat liver tissues. Cells at passage 0 are cryo-preserved and each vial contains 3x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. Rat Primary Hepatocytes are characterized by immunofluorescence staining with an antibody of ZO-1. Cells can be plated on culture plates for desired experiments under the cell culture conditions specified by Cell Biologics. Primary hepatocytes can not be cultured indefinitely and can not be passaged.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-150°C) immediately until ready for use. Cells should be plated and used directly for desired studies within 1-2 weeks upon receipt. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRat Primary Hepatocytes from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e_____________________________________________________________________________________\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Hepatocyte Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a bio-safety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eReview the information provided on the Cell Biologics website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture media (30-50 ML, Catalog No. M1365) to seed cells and when changing media.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCoating of Cell Culture Plates or Dishes\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCoat sterile culture dishes or flasks with Gelatin Coating Solution (Catalog No. 6950, Cell Biologics) for 2 min, then aspirate the excess solution.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell Recovery from Cryovial\u003c\/strong\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003e Thaw the cells quickly (\u0026lt;2 min) in a 37°C water bath until just prior to complete thawing.\u003c\/li\u003e\n\u003cli\u003e Wipe the outside of the vial with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Gently resuspend the cells and transfer cells (3 million cells) to a 7 ml sterile tube.\u003c\/li\u003e\n\u003cli\u003e Add 7 ml of pre-warmed Cell Biologics’ Cell Culture Medium (Catalog number M1365, Cell Biologics) gently to the side of the tube and slowly pipette up and down 2 to 3 times to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003e Carefully pour cell suspension into 100 mm dish (0.6-1.0 million cells per well of 6-well plate).\u003c\/li\u003e\n\u003cli\u003e Once the cells have attached to the culture dishes about 70-80% (after 2-8h), gently aspirate supernatant (remove cell debris and non-adherent cells) and replace the pre-warmed Maintenance Medium (Catalog number M1365, Cell Biologics).\u003c\/li\u003e\n\u003cli\u003e Cells should be checked daily under a microscope to verify appropriate cell morphology.\u003c\/li\u003e\n\u003cli\u003e Proceed with experiment assays within 1-3 days (when cells have reached 90-100% confluence) after plating cells.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cp\u003eNote: The numbers of seeding cells in each well may need to be modified according to the user's experience. Recommended seeding density: 200,000 viable cells\/cm\u003csup\u003e2\u003c\/sup\u003e.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (3 x 10^6 cells)","offer_id":54527900778780,"sku":"RA-6224","price":560.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-1ff9789d53.jpg?v=1791766978"},{"product_id":"rat-liver-kupffer-cells","title":"Rat Liver Kupffer Cells","description":"\u003cp\u003e\u003cstrong\u003eRat Primary Liver Kupffer Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. RA-6226S \u003cb\u003e(Live cells in a plate\/wells only)\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM3368 Complete Macrophage Medium \/w Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRat Primary Liver Kupffer Cells from Cell Biologics are isolated from Sprague-Dawley rat liver tissues and grown in tissue culture plates with Cell Biologics’ Cell Culture Medium. Live Cells at passage 0 in culture plates\/wells or flasks are shipped and cells are ready used for experiments. Cells are characterized by immunofluorescence staining using an antibody of CD11b. Primary Liver Kupffer Cells can not be cultured indefinitely and can not be passaged.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eHandling of Arriving Live Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLive Cells at passage 0 in culture plates or flasks are shipped overnight. When you receive the live cells in plates or flasks, remove the sticker from the filter cap, and keep the plates or flasks with 3-15 ml existing medium in a 37°C CO\u003csub\u003e2\u003c\/sub\u003e incubator for 1 hour before replacing the desired Cell Biologics' cell culture medium.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRat Primary Liver Kupffer Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e__________________________________________________________________________________\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Liver Kupffer Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a bio-safety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eReview the information provided on the Cell Biologics website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture media (30-50 ML, Catalog No. M3368) to seed cells and when changing media.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eHandling of Arriving Live Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLive Cells at passage 0 in a culture plate are shipped overnight. When you receive the live cells in a plate or flask, remove the sticker from the filter cap, and keep the plate or flask with 6-15 ml of existing medium in a 37°C CO\u003csub\u003e2\u003c\/sub\u003e incubator for 1 hour before replacing the desired Cell Biologics' cell culture medium.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e\u003cu\u003eHandling of Arriving Live Cells\u003c\/u\u003e\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLive Cells at passage 0 in a culture plate or flask are shipped overnight. When you receive the live cells in a plate or flask, keep the plate or flask with suggested medium volumn in 37°C CO\u003csup\u003e2\u003c\/sup\u003e incubator for 1 hour before replacing the desired Cell Biologics' cell culture medium.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eNote: A recommended media volume for culture dishes, plates and flasks:\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e6-well plate: Use 2 ml medium per well;\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e12-well plate: Use 0.5 ml medium per well;\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e96-well: Use 100 ul medium per well;\u003cbr\u003e\u003c\/p\u003e\u003cp\u003eCells in T25 flask; Use 5ml medium;\u003cbr\u003e\u003c\/p\u003e\u003cp\u003eCells in T75 flask: Use 10 ml medium\u003c\/p\u003e\u003cp\u003eProceed with experiment assays.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"6 wells","offer_id":54527901237532,"sku":"RA-6226S","price":760.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687906595100,"sku":"RA-6226S","price":820.0,"currency_code":"USD","in_stock":true},{"title":"12 wells","offer_id":54687906627868,"sku":"RA-6226S","price":760.0,"currency_code":"USD","in_stock":true},{"title":"24 wells","offer_id":54687906660636,"sku":"RA-6226S","price":760.0,"currency_code":"USD","in_stock":true},{"title":"96 wells","offer_id":54687906693404,"sku":"RA-6226S","price":760.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ebbd01e18e.jpg?v=1791767016"}],"url":"https:\/\/jkpgye-36.myshopify.com\/collections\/rat-hepatocytes-stellate-cells-kupffer-cells.oembed","provider":"Cell Biologics","version":"1.0","type":"link"}