{"title":"Porcine Primary Hepatocytes, Stellate Cells, Kupffer Cells","description":"\u003cp\u003eCustom Cell Isolation (Customer will need to provide\/purchase tissues for cell isolation).\u003c\/p\u003e\u003cp\u003ePrimary Hepatocytes should be used for experiments within a week after received.\u003c\/p\u003e","products":[{"product_id":"porcine-primary-hepatocytes-pleatable","title":"Porcine Primary Hepatocytes - Pleatable","description":"\u003cp\u003e\u003cb\u003ePorcine Primary Hepatocytes -Pleatable\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eP-6224F \u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCustom Cell Isolation for more than 3 vials. \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Hepatocyte Medium \/w Kit (125 ml)\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eM1365\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003ePorcine Primary Hepatocytes are derived from the the American Landrace Swine liver. Cells are freshly isolated and shipped in 10-50ml cell culture medium overnight at 4°C. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Porcine Primary Hepatocytes are tested for expression of markers using antibodies, ZO-1 by Immunofluorescence Staining. Cells can be plated on culture plates for desired experiments under the cell culture conditions specified by Cell Biologics. Primary hepatocytes can not be cultured indefinitely and can not be passaged. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eLaboratory Applications\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003ePorcine Primary Hepatocytes can be used in standard biochemical procedures include PCR, Western blotting, immunoprecipitation, metabolism, drug-drug interaction, drug transporter activity toxicity of drug candidates or cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e' Cell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics\u003c\/i\u003e' cell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics\u003c\/i\u003e' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003ePorcine Primary Hepatocytes from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for \u003ci\u003ein vitro\u003c\/i\u003e diagnostic procedures, or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics'\u003c\/i\u003e Cells or Products from the purchaser to other markets, organizations, or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Hepatocyte Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a bio-safety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eReview the information provided on the Cell Biologics website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture media (30-50 ML, Catalog No. M1365) to seed cells and when changing media.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell Recovery from Cryovial\u003c\/strong\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003e Thaw the cells quickly (\u0026lt;2 min) in a 37°C water bath until just prior to complete thawing.\u003c\/li\u003e\n\u003cli\u003e Wipe the outside of the vial with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Gently resuspend the cells and transfer cells (3 million cells) to a 7 ml sterile tube.\u003c\/li\u003e\n\u003cli\u003e Add 7 ml of pre-warmed Cell Biologics’ Cell Culture Medium (Catalog number M1365, Cell Biologics) gently to the side of the tube and slowly pipette up and down 2 to 3 times to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003e Carefully pour cell suspension into 100 mm dish (0.6-1.0 million cells per well of 6-well plate).\u003c\/li\u003e\n\u003cli\u003e Once the cells have attached to the culture dishes about 70-80% (after 2-8h), gently aspirate supernatant (remove cell debris and non-adherent cells) and replace the pre-warmed Maintenance Medium (Catalog number M1365, Cell Biologics).\u003c\/li\u003e\n\u003cli\u003e Cells should be checked daily under a microscope to verify appropriate cell morphology.\u003c\/li\u003e\n\u003cli\u003e Proceed with experiment assays within 1-3 days (when cells have reached 90-100% confluence) after plating cells.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cp\u003eNote: The numbers of seeding cells in each well may need to be modified according to the user's experience. Recommended seeding density: 200,000 viable cells\/cm\u003csup\u003e2\u003c\/sup\u003e.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (6 x 10^6 cells)","offer_id":54527861227804,"sku":"P-6224","price":1200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-e00463856a.jpg?v=1791766977"}],"url":"https:\/\/jkpgye-36.myshopify.com\/collections\/porcine-primary-hepatocytes-stellate-cells-kupffer-cells.oembed","provider":"Cell Biologics","version":"1.0","type":"link"}