{"title":"Pancreatic Islets (Animal and Human)","description":"\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e provides custom services to our customers upon request.\u003c\/p\u003e\u003cp\u003eIf you have any special needs in cell isolation and other cell products of Islet DNA, islet RNA, Total insulin, Acinal cells, please contact us: \u003ca href=\"mailto:service@cellbiologics.com\"\u003eservice@cellbiologics.com.\u003c\/a\u003e\u003c\/p\u003e","products":[{"product_id":"c57bl-6-mouse-pancreatic-islets-c57-6232s","title":"C57BL\/6 Mouse Pancreatic Islets","description":"\u003cp\u003e\u003cb\u003eC57BL\/6 Mouse Pancreatic Islets \u003c\/b\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1653671571.pdf\" target=\"_blank\" rel=\"noopener\"\u003e\u003cu\u003e\u003cb\u003eSpecial Order Form\u003c\/b\u003e\u003c\/u\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eC57-6232L (Live Cells) or C57-6232F (Frozen vial) \u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Pancreatic islet culture medium \/w Kit – 100 ML\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eM2269 \u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Pancreatic Islets from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from the pancreas of C57BL\/6 mice using Collagenase P and purified using Ficoll density gradient. The isolated islets are cultured in \u003ci\u003eCell Biologics’\u003c\/i\u003e Pancreatic Islet Culture Medium containing 5% FBS.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Pancreatic Islets are tested for their viability using FDA\/PI, purity using Dithizone staining, static insulin secretion in response to 14 mM glucose. Upon request, dynamic insulin secretion and calcium imaging in response to glucose and other stimulators can be performed.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Pancreatic Islets can be used for the assay of islet function, drug testing, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or others for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships fresh islet cells on at either room temperature or 4°C. On receipt, immediately transfer the cells to 37°C and 5% CO\u003csub\u003e2\u003c\/sub\u003e incubator.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Pancreatic Islets from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’ \u003c\/i\u003eonline protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePrimary Cell Culture Protocol\u003c\/b\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e All cell culture procedures must be conducted in a bio-safety cabinet.\u003c\/li\u003e\n\u003cli\u003e Any and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/li\u003e\n\u003cli\u003e Use aseptic technique to prevent microbial contamination.\u003c\/li\u003e\n\u003cli\u003e Cryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cb\u003eMedium:\u003c\/b\u003e\u003c\/p\u003e\u003cul\u003e\u003cli\u003e Review the information provided on the \u003ci\u003eCell Biologics\u003c\/i\u003e website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture media (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003e\u003cb\u003eHandling of Arriving Live Cells\u003c\/b\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e When you receive the live cells in a tube, keep cells in 37°C CO\u003csub\u003e2\u003c\/sub\u003e incubator for 15 min.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 500 rpm for 1 minute.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in 6 ml of \u003ci\u003eCell Biologics\u003c\/i\u003e’ Cell Culture Growth medium (Catalog No. M2269).\u003c\/li\u003e\n\u003cli\u003e Transfer cells from a vial to the cell culture plate.\u003c\/li\u003e\n\u003cli\u003e Place a plate in a humidified, 5%-CO\u003csub\u003e2\u003c\/sub\u003e incubator at 37°C until experiments.\u003c\/li\u003e\n\u003cli\u003e Cells can be cultured for 3-7 days.\u003c\/li\u003e\n\u003cli\u003e Change fresh cell culture medium every 24-48 hours.\u003c\/li\u003e\n\u003cli\u003e Cells should be checked daily under a microscopy to verify appropriate cell morphology.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"100 islets (Cells in suspension)","offer_id":54527570084124,"sku":"C57-6232S","price":510.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-c3c8a4aaed.jpg?v=1791766977"},{"product_id":"canine-pancreatic-islets","title":"Canine Pancreatic Islets","description":"\u003cp\u003e\u003cb\u003eCanine Pancreatic Islets \u003c\/b\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1653671571.pdf\" target=\"_blank\" rel=\"noopener\"\u003e\u003cu\u003e\u003cb\u003eSpecial Order Form \u003c\/b\u003e\u003c\/u\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. D\u003cb\u003e-6232L (Live Cells) or D-6232F (Frozen vial)\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Pancreatic islet culture medium \/w Kit – 100 ML\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eM2269\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCanine Pancreatic Islets from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from the beagle dog pancreas using Collagenase P and purified using Ficoll density gradient. The isolated islets are cultured in \u003ci\u003eCell Biologics’\u003c\/i\u003e Pancreatic Islet Culture Medium containing 5% FBS.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCanine Pancreatic Islets are tested for their viability using FDA\/PI, purity using Dithizone staining, static insulin secretion in response to 14 mM glucose. Upon request, dynamic insulin secretion and calcium imaging in response to glucose and other stimulators can be performed.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCanine Pancreatic Islets can be used for the assay of islet function, drug testing, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or others for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships fresh islet cells on at either room temperature or 4°C. On receipt, immediately transfer the cells to 37°C and 5% CO\u003csub\u003e2\u003c\/sub\u003e incubator.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCanine Pancreatic Islets from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’ \u003c\/i\u003eonline protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePrimary Cell Culture Protocol\u003c\/b\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e All cell culture procedures must be conducted in a bio-safety cabinet.\u003c\/li\u003e\n\u003cli\u003e Any and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/li\u003e\n\u003cli\u003e Use aseptic technique to prevent microbial contamination.\u003c\/li\u003e\n\u003cli\u003e Cryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cb\u003eMedium:\u003c\/b\u003e\u003c\/p\u003e\u003cul\u003e\u003cli\u003e Review the information provided on the \u003ci\u003eCell Biologics\u003c\/i\u003e website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture media (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003e\u003cu\u003e\u003cb\u003eHandling of Arriving Live Cells\u003c\/b\u003e\u003c\/u\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e When you receive the live cells in a tube, keep cells in 37°C CO\u003csub\u003e2\u003c\/sub\u003e incubator for 15 min.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 500 rpm for 1 minute.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in 6 ml of \u003ci\u003eCell Biologics\u003c\/i\u003e’ Cell Culture Growth medium (Catalog No. M2269).\u003c\/li\u003e\n\u003cli\u003e Transfer cells from a vial to the cell culture plate.\u003c\/li\u003e\n\u003cli\u003e Place a plate in a humidified, 5%-CO\u003csub\u003e2\u003c\/sub\u003e incubator at 37°C until experiments.\u003c\/li\u003e\n\u003cli\u003e Cells can be cultured for 3-7 days.\u003c\/li\u003e\n\u003cli\u003e Change fresh cell culture medium every 24-48 hours.\u003c\/li\u003e\n\u003cli\u003e Cells should be checked daily under a microscopy to verify appropriate cell morphology.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"1000 islets (Cells in suspension)","offer_id":54527628345628,"sku":"D-6232S","price":8000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-e92f8ad4c8.jpg?v=1791766977"},{"product_id":"rabbit-pancreatic-islets","title":"Rabbit Pancreatic Islets","description":"\u003cp\u003e\u003cb\u003eRabbit Pancreatic Islets \u003c\/b\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1653671571.pdf\" target=\"_blank\" rel=\"noopener\"\u003e\u003cu\u003e\u003cb\u003eSpecial Order Form\u003c\/b\u003e\u003c\/u\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cstrong\u003eN\u003c\/strong\u003e\u003cb\u003e\u003cstrong\u003e-6232L\u003c\/strong\u003e (Live Cells) or N-6232F (Frozen vial)\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Pancreatic islet culture medium \/w Kit – 100 ML\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eM2269\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eRabbit Pancreatic Islets from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from the New Zealand White Rabbit pancreas using Collagenase P and purified using Ficoll density gradient. The isolated islets are cultured in \u003ci\u003eCell Biologics’\u003c\/i\u003e Pancreatic Islet Culture Medium containing 5% FBS.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eRabbit Pancreatic Islets are tested for their viability using FDA\/PI, purity using Dithizone staining, static insulin secretion in response to 14 mM glucose. Upon request, dynamic insulin secretion and calcium imaging in response to glucose and other stimulators can be performed.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eRabbit Pancreatic Islets can be used for the assay of islet function, drug testing, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or others for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships fresh islet cells on at either room temperature or 4°C. On receipt, immediately transfer the cells to 37°C and 5% CO\u003csub\u003e2\u003c\/sub\u003e incubator.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eRabbit Pancreatic Islets from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’ \u003c\/i\u003eonline protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePrimary Cell Culture Protocol\u003c\/b\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e All cell culture procedures must be conducted in a bio-safety cabinet.\u003c\/li\u003e\n\u003cli\u003e Any and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/li\u003e\n\u003cli\u003e Use aseptic technique to prevent microbial contamination.\u003c\/li\u003e\n\u003cli\u003e Cryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cb\u003eMedium:\u003c\/b\u003e\u003c\/p\u003e\u003cul\u003e\u003cli\u003e Review the information provided on the \u003ci\u003eCell Biologics\u003c\/i\u003e website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture media (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003e\u003cu\u003e\u003cb\u003eHandling of Arriving Live Cells\u003c\/b\u003e\u003c\/u\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e When you receive the live cells in a tube, keep cells in 37°C CO\u003csub\u003e2\u003c\/sub\u003e incubator for 15 min.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 500 rpm for 1 minute.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in 6 ml of \u003ci\u003eCell Biologics\u003c\/i\u003e’ Cell Culture Growth medium (Catalog No. M2269).\u003c\/li\u003e\n\u003cli\u003e Transfer cells from a vial to the cell culture plate.\u003c\/li\u003e\n\u003cli\u003e Place a plate in a humidified, 5%-CO\u003csub\u003e2\u003c\/sub\u003e incubator at 37°C until experiments.\u003c\/li\u003e\n\u003cli\u003e Cells can be cultured for 3-7 days.\u003c\/li\u003e\n\u003cli\u003e Change fresh cell culture medium every 24-48 hours.\u003c\/li\u003e\n\u003cli\u003e Cells should be checked daily under a microscopy to verify appropriate cell morphology.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"1000 islets (Cells in suspension)","offer_id":54527821218076,"sku":"N-6232S","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-e92f8ad4c8.jpg?v=1791766977"},{"product_id":"porcine-pancreatic-islets","title":"Porcine Pancreatic Islets","description":"\u003cp\u003e\u003cb\u003ePorcine Pancreatic Islets \u003c\/b\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1653671571.pdf\" target=\"_blank\" rel=\"noopener\"\u003e\u003cu\u003e\u003cb\u003eSpecial Order Form\u003c\/b\u003e\u003c\/u\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eP-6232L (Live Cells)\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eP-6232F (Frozen vial)\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Pancreatic islet culture medium \/w Kit – 100 ML\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eM2269\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003ePorcine Pancreatic Islets from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from the American Landrace Swine pancreas using Collagenase P and purified using Ficoll density gradient. The isolated islets are cultured in \u003ci\u003eCell Biologics’\u003c\/i\u003e Pancreatic Islet Culture Medium containing 5% FBS.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003ePorcine Pancreatic Islets are tested for their viability using FDA\/PI, purity using Dithizone staining, static insulin secretion in response to 14 mM glucose. Upon request, dynamic insulin secretion and calcium imaging in response to glucose and other stimulators can be performed.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003ePorcine Pancreatic Islets can be used for the assay of islet function, drug testing, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or others for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships fresh islet cells on at either room temperature or 4°C. On receipt, immediately transfer the cells to 37°C and 5% CO\u003csub\u003e2\u003c\/sub\u003e incubator.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003ePorcine Pancreatic Islets from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’ \u003c\/i\u003eonline protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePrimary Cell Culture Protocol\u003c\/b\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e All cell culture procedures must be conducted in a bio-safety cabinet.\u003c\/li\u003e\n\u003cli\u003e Any and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/li\u003e\n\u003cli\u003e Use aseptic technique to prevent microbial contamination.\u003c\/li\u003e\n\u003cli\u003e Cryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cb\u003eMedium:\u003c\/b\u003e\u003c\/p\u003e\u003cul\u003e\u003cli\u003e Review the information provided on the \u003ci\u003eCell Biologics\u003c\/i\u003e website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture media (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003e\u003cu\u003e\u003cb\u003eHandling of Arriving Live Cells\u003c\/b\u003e\u003c\/u\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e When you receive the live cells in a tube, keep cells in 37°C CO\u003csub\u003e2\u003c\/sub\u003e incubator for 15 min.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 500 rpm for 1 minute.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in 6 ml of \u003ci\u003eCell Biologics\u003c\/i\u003e’ Cell Culture Growth medium (Catalog No. M2269).\u003c\/li\u003e\n\u003cli\u003e Transfer cells from a vial to the cell culture plate.\u003c\/li\u003e\n\u003cli\u003e Place a plate in a humidified, 5%-CO\u003csub\u003e2\u003c\/sub\u003e incubator at 37°C until experiments.\u003c\/li\u003e\n\u003cli\u003e Cells can be cultured for 3-7 days.\u003c\/li\u003e\n\u003cli\u003e Change fresh cell culture medium every 24-48 hours.\u003c\/li\u003e\n\u003cli\u003e Cells should be checked daily under a microscopy to verify appropriate cell morphology.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eIf you have any special needs in cell isolation and other cell products of Islet DNA, islet RNA, Total insulin, Acinal cells, please fill out the \u003ca href=\"https:\/\/cellbiologics.com\/document\/1653671571.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSpecial Order Form\u003c\/a\u003e and contact us: \u003ca href=\"mailto:service@cellbiologics.com\"\u003eservice@cellbiologics.com.\u003c\/a\u003e\u003c\/p\u003e\u003ctable\u003e\u003c\/table\u003e","brand":"Cell Biologics","offers":[{"title":"5000 Islets (Cells in suspension)","offer_id":54527862047004,"sku":"P-6232S","price":8000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-e92f8ad4c8.jpg?v=1791766977"}],"url":"https:\/\/jkpgye-36.myshopify.com\/collections\/pancreatic-islets-animal-and-human.oembed","provider":"Cell Biologics","version":"1.0","type":"link"}