{"title":"Neutrophils","description":null,"products":[{"product_id":"aged-mouse-bone-marrow-neutrophils","title":"Aged Mouse Bone Marrow Neutrophils","description":"\u003cp\u003eAged Mouse Bone Marrow Neutrophils - Aged Mouse primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527518572828,"sku":"A57-6029","price":637.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687243501852,"sku":"A57-6029","price":850.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687243534620,"sku":"A57-6029","price":1062.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-7c89b4d443.jpg?v=1791766977"},{"product_id":"b129-mouse-bone-marrow-neutrophils","title":"B129 Mouse Bone Marrow Neutrophils","description":"\u003cp\u003eB129 Mouse Bone Marrow Neutrophils - Mouse primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527525716252,"sku":"B129-7029","price":510.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-7c89b4d443.jpg?v=1791766977"},{"product_id":"balb-c-mouse-bone-marrow-neutrophils-frozen-cells","title":"BALB\/c Mouse Bone Marrow Neutrophils (Frozen Cells)","description":"\u003cp\u003e\u003cstrong\u003eBALB\/c Mouse Bone Marrow Neutrophils \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. BALB-5029F\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM3367 Complete Neutrophil Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Bone Marrow Neutrophils from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory BALB\/c mice. The cells at passage 0 are cryo-preserved, each vial contains 5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. These cells can be used for designed experiments under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Bone Marrow Neutrophils from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e_______________________________________________________________________________________\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003eMedium: Use pre-warmed (37°C) cell culture media of Catalog No. M3367 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003eCell recovery from cryovial:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527529353500,"sku":"BALB-5029","price":510.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-74b86a7a2f.jpg?v=1791767016"},{"product_id":"c57bl-6-mouse-bone-marrow-neutrophils","title":"C57BL\/6 Mouse Bone Marrow Neutrophils","description":"\u003cp\u003e\u003cstrong\u003eC57BL\/6 Mouse Bone Marrow Neutrophils \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6029F\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM3366 Complete Leukocyte Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Bone Marrow Neutrophils from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory adult mice. Cells at passage 0 are cryo-preserved and each vial contains 5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. Cells in suspension culturecan be used for designed experiments under the cell culture conditions specified by Cell Biologics. Cells can be used in standard biochemical procedures include FACS, PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications. Cells are negative for bacteria, yeast, fungi, and mycoplasma. These cells will not proliferate in culture and cannot be passaged.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Bone Marrow Neutrophils from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e_____________________________________________________________________________________\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium:\u003c\/strong\u003e Use pre-warmed (37°C) cell culture media of Catalog No. M7711 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell recovery from cryovial:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527540396316,"sku":"C57-6029","price":510.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-7c89b4d443.jpg?v=1791766977"},{"product_id":"canine-neutrophils","title":"Canine Neutrophils","description":"\u003cp\u003eCanine Neutrophils - Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527571460380,"sku":"CABM.Neutrophils","price":1600.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-label-cabm.neutrophils.jpg?v=1791821715"},{"product_id":"canine-neutrophils-capb-neutrophils","title":"Canine Neutrophils","description":"\u003cp\u003eCanine Neutrophils - Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527572115740,"sku":"CAPB.Neutrophils","price":1350.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-label-capb.neutrophils.jpg?v=1791821715"},{"product_id":"cd1-mouse-bone-marrow-neutrophils","title":"CD1 Mouse Bone Marrow Neutrophils","description":"\u003cp\u003e\u003cstrong\u003eCD1 Mouse Bone Marrow Neutrophils \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. CD-1029F\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM3367 Complete Neutrophil Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCD1 Mouse Bone Marrow Neutrophils from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory CD1 mice. The cells at passage 0 are cryo-preserved, each vial contains 5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. These cells can be used for designed experiments under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCD1 Mouse Bone Marrow Neutrophils from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e_______________________________________________________________________________________\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003eMedium: Use pre-warmed (37°C) cell culture media of Catalog No. M3367 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003eCell recovery from cryovial:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527573623068,"sku":"CD-1029","price":510.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-7c89b4d443.jpg?v=1791766977"},{"product_id":"human-neutrophils","title":"Human Neutrophils","description":"\u003cp\u003eHuman Neutrophils - Human primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527710986524,"sku":"HUBM.Neutrophils","price":1850.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-label-hubm.neutrophils.jpg?v=1791821715"},{"product_id":"human-neutrophils-hupb-neutrophils","title":"Human Neutrophils","description":"\u003cp\u003eHuman Neutrophils - Human primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527711772956,"sku":"HUPB.Neutrophils","price":1600.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-label-hupb.neutrophils.jpg?v=1791821715"},{"product_id":"bks-db-control-mouse-bone-marrow-neutrophils","title":"BKS db Control Mouse Bone Marrow Neutrophils","description":"\u003cp\u003eBKS db Control Mouse Bone Marrow Neutrophils - BKS db Mouse primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527713935644,"sku":"K57-6029","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687855280412,"sku":"K57-6029","price":850.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687855313180,"sku":"K57-6029","price":1062.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-7c89b4d443.jpg?v=1791766977"},{"product_id":"diabetic-mouse-bone-marrow-neutrophils","title":"Diabetic Mouse Bone Marrow Neutrophils","description":"\u003cp\u003eDiabetic Mouse Bone Marrow Neutrophils - Mouse primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527721111836,"sku":"MD-6029","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687865176348,"sku":"MD-6029","price":850.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687865209116,"sku":"MD-6029","price":1062.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-7c89b4d443.jpg?v=1791766977"},{"product_id":"cynomolgus-monkey-bone-marrow-neutrophils-cells-frozen-vial","title":"Cynomolgus Monkey Bone Marrow Neutrophils Cells Frozen Vial","description":"\u003cp\u003e\u003cstrong\u003eMonkey Bone Marrow Neutrophils \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. MK-6029F\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM3367 Complete Neutrophil Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eMonkey Bone Marrow Neutrophils from Cell Biologics are isolated from Cynomolgus monkey tibias and femurs. The cells at passage 0 are cryo-preserved, each vial contains 5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Cells in suspension culturecan be used for designed experiments under the cell culture conditions specified by Cell Biologics. Cells can be used in standard biochemical procedures include FACS, PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications. Cells are negative for bacteria, yeast, fungi, and mycoplasma. These cells will not proliferate in culture and cannot be passaged.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eMonkey Bone Marrow Neutrophils from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e_______________________________________________________________________________________\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003eMedium: Use pre-warmed (37°C) cell culture media of Catalog No. M3367 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003eCell recovery from cryovial:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527737364764,"sku":"MK-6029","price":650.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-7c89b4d443.jpg?v=1791766977"},{"product_id":"monkey-neutrophils","title":"Monkey Neutrophils","description":"\u003cp\u003eMonkey Neutrophils - Monkey primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527773278492,"sku":"MKBM.Neutrophils","price":1850.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-label-mkbm.neutrophils.jpg?v=1791821715"},{"product_id":"monkey-neutrophils-mkpb-neutrophils","title":"Monkey Neutrophils","description":"\u003cp\u003eMonkey Neutrophils - Monkey primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527774032156,"sku":"MKPB.Neutrophils","price":1600.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-label-mkpb.neutrophils.jpg?v=1791821716"},{"product_id":"mouse-neutrophils","title":"Mouse Neutrophils","description":"\u003cp\u003eMouse Neutrophils - Mouse primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527775244572,"sku":"MSBM.Neutrophils","price":850.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-label-msbm.neutrophils.jpg?v=1791821818"},{"product_id":"mouse-neutrophils-mspb-neutrophils","title":"Mouse Neutrophils","description":"\u003cp\u003eMouse Neutrophils - Mouse primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527775801628,"sku":"MSPB.Neutrophils","price":725.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-label-mspb.neutrophils.jpg?v=1791821817"},{"product_id":"porcine-neutrophils","title":"Porcine Neutrophils","description":"\u003cp\u003ePorcine Neutrophils - Porcine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527862505756,"sku":"POBM.Neutrophils","price":1600.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-label-pobm.neutrophils.jpg?v=1791821818"},{"product_id":"porcine-neutrophils-popb-neutrophils","title":"Porcine Neutrophils","description":"\u003cp\u003ePorcine Neutrophils - Porcine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527863030044,"sku":"POPB.Neutrophils","price":1350.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-label-popb.neutrophils.jpg?v=1791821818"},{"product_id":"rat-bone-marrow-neutrophils-frozen-cells","title":"Rat Bone Marrow Neutrophils (Frozen Cells)","description":"\u003cp\u003e\u003cb\u003eRat Bone Marrow Neutrophils- Frozen Vial (\u003c\/b\u003e\u003cb\u003eIn Suspension Culture)\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. RA-6029F\u003c\/p\u003e\u003cp\u003eSuggested Medium: Neutrophil Medium \/w Kit (500 ml) \u003c\/p\u003e\u003cp\u003eCatalog No. M3367\u003c\/p\u003e\u003cp\u003eProduct Description\u003c\/p\u003e\u003cp\u003eRat Bone Marrow Neutrophils are derived from the tibias of Sprague Dawley Rat. Each vial contains 5x10\u003csup\u003e6\u003c\/sup\u003e cells and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells in suspension culturecan be used for designed experiments under the cell culture conditions specified by Cell Biologics. Cells can be used in standard biochemical procedures include FACS, PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications. Cells are negative for bacteria, yeast, fungi, and mycoplasma. These cells will not proliferate in culture and cannot be passaged.\u003c\/p\u003e\u003cp\u003eLaboratory Applications\u003c\/p\u003e\u003cp\u003eRat Bone Marrow Neutrophils can be used in standard biochemical procedures include PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003eStorage of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e will ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use. Live-cell shipment is also available on request. Primary cells can never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003eAuthorized Uses of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/p\u003e\u003cp\u003eRat Bone Marrow Neutrophils from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for \u003ci\u003ein vitro\u003c\/i\u003e diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics,\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003eDisclaimer\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all Cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003eWarranty and Liability\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527871648028,"sku":"RA-6029","price":560.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-74b86a7a2f.jpg?v=1791767016"},{"product_id":"rabbit-neutrophils","title":"Rabbit Neutrophils","description":"\u003cp\u003eRabbit Neutrophils - Rabbit primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527903760668,"sku":"RBTBM.Neutrophils","price":1600.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-label-rbtbm.neutrophils.jpg?v=1791821816"},{"product_id":"rabbit-neutrophils-rbtpb-neutrophils","title":"Rabbit Neutrophils","description":"\u003cp\u003eRabbit Neutrophils - Rabbit primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527904416028,"sku":"RBTPB.Neutrophils","price":1350.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-label-rbtpb.neutrophils.jpg?v=1791821818"},{"product_id":"rat-neutrophils","title":"Rat Neutrophils","description":"\u003cp\u003eRat Neutrophils - Rat primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527910183196,"sku":"RTBM.Neutrophils","price":850.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-label-rtbm.neutrophils.jpg?v=1791821818"},{"product_id":"rat-neutrophils-rtpb-neutrophils","title":"Rat Neutrophils","description":"\u003cp\u003eRat Neutrophils - Rat primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527910936860,"sku":"RTPB.Neutrophils","price":725.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-label-rtpb.neutrophils.jpg?v=1791821818"},{"product_id":"complete-neutrophil-medium-w-kit","title":"Complete Neutrophil Medium \/w Kit","description":"\u003cp\u003e\u003cstrong\u003eComplete Neutrophil Medium \/w Kit – 500 ML\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No: M3367\u003c\/p\u003e\u003cp\u003e(500 ml Basal medium with M3367-Kit)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eNeutrophil Medium is a complete medium designed for the culture of neutrophils. It was tested and optimized with neutrophil culture in vitro. It is formulated for use with 5% CO\u003csub\u003e2\u003c\/sub\u003e and 95% air in a humidified incubator.\u003c\/p\u003e\u003cp\u003eThe medium consists of 500 ml of basal medium (containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals), supplemented with neutrophil culture supplement, antibiotics, and fetal bovine serum.\u003c\/p\u003e\u003cp\u003eNeutrophil Medium Supplement Kit, Cat. No. M3367-Kit includes:\u003c\/p\u003e\u003cp\u003e- 5.0 ML Penicillin-Streptomycin\u003c\/p\u003e\u003cp\u003e- 25.0 ML FBS\u003c\/p\u003e\u003cp\u003e*Before use, add M3367-Kit to 500ml basal medium.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage Condition\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eStore the basal medium at 2-8°C. Store cell growth supplement, fetal bovine serum (FBS) and antibiotics at -20°C. The complete cell culture medium with Supplement Kit can be kept in 4°C for two months. Protect from light.\u003c\/p\u003e\u003cp\u003eTo assure sterility, the prepared medium should be filtered with a 0.2 um filter after 2 weeks or if there is concern that sterility was compromised during the supplementation process.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eShipping\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eIce pack and dry ice.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of \u003cem\u003eCell Biologics \u003c\/em\u003eProducts\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eNeutrophil Media from \u003cem\u003eCell Biologics\u003c\/em\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for \u003cem\u003ein vitro\u003c\/em\u003e diagnostic procedures, or for therapeutic procedures. Transfer or resale of any \u003cem\u003eCell Biologics’\u003c\/em\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003cem\u003eCell Biologics\u003c\/em\u003e without the company’s written consent. \u003cem\u003eCell Biologics’\u003c\/em\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"500 ML","offer_id":54527917654300,"sku":"M3367","price":128.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-7c04567a23.jpg?v=1791767087"}],"url":"https:\/\/jkpgye-36.myshopify.com\/collections\/neutrophils.oembed","provider":"Cell Biologics","version":"1.0","type":"link"}