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Primary cells can never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eB129 Mouse Bone Marrow Macrophages from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for \u003ci\u003ein vitro\u003c\/i\u003e diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e, without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all Cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (3 x 10^6 cells)","offer_id":54527525749020,"sku":"B129-7030","price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-b937ca7319.jpg?v=1791767016"},{"product_id":"b129-mouse-peritoneal-macrophages-thioglycollate-elicited-frozen-cells","title":"B129 Mouse Peritoneal Macrophages - Thioglycollate-elicited (Frozen Cells)","description":"\u003cp\u003e\u003cb\u003eB129 Mouse Peritoneal Macrophages - Thioglycollate-elicited (Frozen Cells)\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eB129-7032TF\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Macrophage Medium \/w Kit (500 ml) \u003c\/p\u003e\u003cp\u003e\u003cb\u003eCatalog No. M3368\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eB129 Mouse Peritoneal Macrophages (Thioglycollate-elicited) are isolated from the peritoneal cavity of pathogen-free laboratory adult mice. 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Primary cells can never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eB129 Mouse Peritoneal Macrophages (Thioglycollate-elicited) from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. 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Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e_____________________________________________________________________________________\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium:\u003c\/strong\u003e Use pre-warmed (37°C) cell culture media of Catalog No. M7711 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell recovery from cryovial:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. 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Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. 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The cells at passage 0 are cryo-preserved, each vial contains 5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. These cells can be used for designed experiments under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Bone Marrow Neutrophils from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e_______________________________________________________________________________________\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003eMedium: Use pre-warmed (37°C) cell culture media of Catalog No. M3367 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003eCell recovery from cryovial:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527529353500,"sku":"BALB-5029","price":510.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-74b86a7a2f.jpg?v=1791767016"},{"product_id":"balb-c-mouse-bone-marrow-macrophages-frozen-cells","title":"BALB\/c Mouse Bone Marrow Macrophages (Frozen Cells)","description":"\u003cp\u003e\u003cstrong\u003eBALB\/c Mouse Bone Marrow Macrophages\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. BALB-5030F\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM3368 Complete Macrophage Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Bone Marrow Macrophages from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory BALB\/c mice and grown in tissue culture plates with Cell Biologics’ Cell Culture Medium. Cells at passage 0 are cryo-preserved and each vial contains 3x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. BALB\/c Mouse Bone Marrow Macrophages are characterized by immunofluorescence staining using antibody of CD11b or F4\/80. These cells can be plated on multiwell culture plates for experiments under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Bone Marrow Macrophages from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e________________________________________________________________________________________\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Macrophage Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a bio-safety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eReview the information provided on the Cell Biologics website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture media (30-50 ML, Catalog No. M3368) to seed cells and when changing media.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCoating of Cell Culture Plates or Dishes\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCoat sterile culture dishes or flasks with Gelatin Coating Solution (Catalog No. 6950, Cell Biologics) for 2 min, then aspirate the excess solution.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell Recovery from Cryovial\u003c\/strong\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003e Thaw the cells quickly (\u0026lt;2 min) in a 37°C water bath until just prior to complete thawing.\u003c\/li\u003e\n\u003cli\u003e Wipe the outside of the vial with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Use 1 ml Pipette Tip to resuspend cells and transfer cells to a 15 ml sterile tube.\u003c\/li\u003e\n\u003cli\u003e Add 5 ml of pre-warmed Cell Biologics’ Cell Culture Medium (Catalog number M3368, Cell Biologics) gently to the side of the tube and slowly pipette up and down 2 to 3 times to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003e Centrifuge the cells at 200 x g at 4°C for 5 min.\u003c\/li\u003e\n\u003cli\u003e Carefully remove the supernatant and resuspend in 7 mL pre-warmed Maintenance Medium (Catalog No. M3368, Cell Biologics).\u003c\/li\u003e\n\u003cli\u003e Carefully pour cell suspension into the cell culture plate or dish.\u003c\/li\u003e\n\u003cli\u003e Recommended Cell Seeding: 0.7-0.8 million cells are seeded per well of a 12-well plate or 1-1.5 million macroPlace a plate in a humidified, 5%-CO\u003csub\u003e2\u003c\/sub\u003e incubator at 37°C until experiments.\u003c\/li\u003e\n\u003cli\u003e Change fresh cell culture medium every 24-48 hours.\u003c\/li\u003e\n\u003cli\u003e Cells should be checked daily under a microscope to verify appropriate cell morphology.\u003c\/li\u003e\n\u003cli\u003e Proceed with experiment assays.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cp\u003eNote: Please send us the cell images (\u0026gt;90% confluence) if you have any question or problem with cultured cells.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (3 x 10^6 cells)","offer_id":54527529386268,"sku":"BALB-5030","price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-36cf55ce90.jpg?v=1791767054"},{"product_id":"balb-c-mouse-peritoneal-macrophages-thioglycollate-elicited-frozen-cells","title":"BALB\/c Mouse Peritoneal Macrophages - Thioglycollate-elicited (Frozen Cells)","description":"\u003cp\u003e\u003cb\u003eBALB\/C Mouse Peritoneal Macrophages- Thioglycollate- elicited- Frozen Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eBALB-5032TF\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Macrophage Medium \/w Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCatalog No. M3368\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eProduct Description\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eMouse Peritoneal Macrophages are derived from the thioglycollate-elicited peritoneal cavity of pathogen-free laboratory adult mice. Mouse Peritoneal Macrophages are negative for bacteria, yeast, fungi, and mycoplasma. Each vial contains at least 6x10\u003csup\u003e6\u003c\/sup\u003e cells and is delivered frozen. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003ePrimary Peritoneal Macrophages can not be cultured indefinitely and can not be passaged. \u003c\/p\u003e\u003cp\u003eMouse Peritoneal Macrophages are tested for expression of markers using antibodies, CD11b by flow cytometry. \u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eLaboratory Applications\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eStandard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eStorage of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e will ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use. Live-cell shipment is also available on request.\u003c\/p\u003e\u003cp\u003eNever can primary cells be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eAuthorized Uses of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eMouse Peritoneal Macrophages from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eDisclaimer\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAlthough Mouse Peritoneal Macrophages are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eWarranty and Liability\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (3 x 10^6 cells)","offer_id":54527529419036,"sku":"BALB-5032TF","price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-36cf55ce90.jpg?v=1791767054"},{"product_id":"balb-c-mouse-bone-marrow-dendritic-cells","title":"BALB\/c Mouse Bone Marrow Dendritic Cells","description":"\u003cp\u003e\u003cstrong\u003eBALB\/c Mouse Bone Marrow Dendritic Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. BALB-5200\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM7711 Complete Dendritic Cell Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Bone Marrow Dendritic Cells from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory BALB\/c mice. Cells at passage 0 are cryo-preserved and each vial contains 1x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. BALB\/c Mouse Bone Marrow Dendritic Cells are characterized by immunofluorescence staining with antibody of CD11c. These cells will not proliferate in culture and cannot be passaged.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Bone Marrow Dendritic Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e_____________________________________________________________________________________\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium:\u003c\/strong\u003e Use pre-warmed (37°C) cell culture media of Catalog No. M7711 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell recovery from cryovial:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (2 x 10^6 cells)","offer_id":54527531909404,"sku":"BALB-5200","price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-b9e283e53d.jpg?v=1791767087"},{"product_id":"balb-c-mouse-bone-marrow-monocytes","title":"BALB\/c Mouse Bone Marrow Monocytes","description":"\u003cp\u003e\u003cstrong\u003eBALB\/c Mouse Bone Marrow Monocytes \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. BALB-5271F\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM3366 Complete Leukocyte Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Bone Marrow Monocytes from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory BALB\/c mice. Cells at passage 0 are cryo-preserved and each vial contains 3 x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. BALB\/c Mouse Bone Marrow Monocytes can be used for designed experiments under the cell culture conditions specified by Cell Biologics. These cells will not proliferate in culture and cannot be passaged.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Bone Marrow Monocytes from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e_____________________________________________________________________________________\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium:\u003c\/strong\u003e Use pre-warmed (37°C) cell culture media of Catalog No. M7711 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell recovery from cryovial:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527533154588,"sku":"BALB-5271","price":760.0,"currency_code":"USD","in_stock":true},{"title":"Frozen Vial 3 x 10^6","offer_id":54687315624220,"sku":"BALB-5271","price":410.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-c3c8a4aaed.jpg?v=1791766977"},{"product_id":"balb-c-mouse-primary-splenocytes","title":"BALB\/c Mouse Primary Splenocytes","description":"\u003cp\u003e\u003cb\u003eBALB\/c Mouse Primary Splenocytes\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. BALB-5321F\u003c\/p\u003e\u003cp\u003eSuggested Medium: Complete Cell Culture Medium\/w Kit - 500 ML\u003cbr\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. M5567\u003c\/p\u003e\u003cp\u003eProduct Description\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Primary Splenocytes are derived from the spleen tissue of pathogen-free laboratory mice. Each vial contains 20 x10\u003csup\u003e6\u003c\/sup\u003e cells and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells are ready for experiments under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended. Cells can be used in standard biochemical procedures include FACS, PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003eStorage of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e will ship frozen cells on dry ice or live cells in suspension at 4 °C. On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use. Live-cell shipment is also available on request. Primary cells can never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003eAuthorized Uses of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Primary Splenocytes from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for \u003ci\u003ein vitro\u003c\/i\u003e diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics,\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003eDisclaimer\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all Cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003eWarranty and Liability\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (10 x 10^6 cells)","offer_id":54527533252892,"sku":"BALB-5321","price":510.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-34b0a0790d.jpg?v=1791766978"},{"product_id":"c57bl-6-mouse-bone-marrow-neutrophils","title":"C57BL\/6 Mouse Bone Marrow Neutrophils","description":"\u003cp\u003e\u003cstrong\u003eC57BL\/6 Mouse Bone Marrow Neutrophils \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6029F\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM3366 Complete Leukocyte Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Bone Marrow Neutrophils from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory adult mice. Cells at passage 0 are cryo-preserved and each vial contains 5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. Cells in suspension culturecan be used for designed experiments under the cell culture conditions specified by Cell Biologics. Cells can be used in standard biochemical procedures include FACS, PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications. Cells are negative for bacteria, yeast, fungi, and mycoplasma. These cells will not proliferate in culture and cannot be passaged.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Bone Marrow Neutrophils from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e_____________________________________________________________________________________\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium:\u003c\/strong\u003e Use pre-warmed (37°C) cell culture media of Catalog No. M7711 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell recovery from cryovial:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527540396316,"sku":"C57-6029","price":510.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-7c89b4d443.jpg?v=1791766977"},{"product_id":"c57bl-6-mouse-bone-marrow-macrophages-frozen-cells","title":"C57BL\/6 Mouse Bone Marrow Macrophages (Frozen Cells)","description":"\u003cstrong\u003eC57BL\/6 Mouse Bone Marrow Macrophages\u003c\/strong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003eCatalog No. C57-6030F\u003c\/strong\u003e\u003cbr\u003e Suggested Medium M3368 Complete Macrophage Medium w\/ Kit (500 ml) \u003cp\u003e\u003cstrong\u003eProduct Description \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Bone Marrow Macrophages from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory C57BL\/6 mice and grown in tissue culture plates with Cell Biologics’ Cell Culture Medium. Cells at passage 0 are cryo-preserved and each vial contains 3x10\u003csup\u003e6 \u003c\/sup\u003ecells per ml. C57BL\/6 Mouse Bone Marrow Macrophages are characterized by immunofluorescence staining using antibody of CD11b or F4\/80. These cells can be plated on multiwell culture plates for experiments under the cell culture conditions specified by Cell Biologics. Cells can not be cultured indefinitely and can not be passaged. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Bone Marrow Macrophages from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order. \u003c\/p\u003e\u003cp\u003eDisclaimer Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. Warranty and Liability \u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Macrophage Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a bio-safety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eMedium Review the information provided on the Cell Biologics website about appropriate culture media (e.g. serum and other supplements).\u003c\/p\u003e\u003cp\u003eUse pre-warmed (37°C) cell culture media (30-50 ML, Catalog No. M3368) to seed cells and when changing media.\u003c\/p\u003e\u003cp\u003eCoating of Cell Culture Plates or Dishes Coat sterile culture dishes or flasks with Gelatin Coating Solution (Catalog No. 6950, Cell Biologics) for 2 min, then aspirate the excess solution.\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell Recovery from Cryovial \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eThaw the cells quickly (\u0026lt;2 min) in a 37°C water bath until just prior to complete thawing.\u003c\/p\u003e\u003cp\u003eWipe the outside of the vial with 70% ethanol.\u003c\/p\u003e\u003cp\u003eUse 1 ml Pipette Tip to resuspend cells and transfer cells to a 15 ml sterile tube.\u003c\/p\u003e\u003cp\u003eAdd 5 ml of pre-warmed Cell Biologics’ Cell Culture Medium (Catalog number M3368, Cell Biologics) gently to the side of the tube and slowly pipette up and down 2 to 3 times to resuspend the cells.\u003c\/p\u003e\u003cp\u003eCentrifuge the cells at 200 x g at 4°C for 5 min.\u003c\/p\u003e\u003cp\u003eCarefully remove the supernatant and resuspend in 7 mL pre-warmed Maintenance Medium (Catalog No. M3368, Cell Biologics).\u003c\/p\u003e\u003cp\u003eCarefully pour cell suspension into the cell culture plate or dish.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRecommended Cell Seeding: \u003c\/strong\u003e0.7-0.8 million cells are seeded per well of a 12-well plate or 1-1.5 million macroPlace a plate in a humidified, 5%-CO\u003csub\u003e2\u003c\/sub\u003e incubator at 37°C until experiments.\u003c\/p\u003e\u003cp\u003eChange fresh cell culture medium every 24-48 hours. Cells should be checked daily under a microscope to verify appropriate cell morphology.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProceed with experiment assays.\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eNote: Please send us the cell images (\u0026gt;90% confluence) if you have any question or problem with cultured cells.\u003c\/p\u003e\u003cp\u003ePublication:\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8263005\/\" target=\"_blank\" rel=\"noopener\"\u003emBio.\u003c\/a\u003e 2021 May-Jun; 12(3): e01227-21.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8769200\/\" target=\"_blank\" rel=\"noopener\"\u003emSphere.\u003c\/a\u003e 2022 Jan-Feb; 7(1): e00934-21.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (3 x 10^6 cells)","offer_id":54527540494620,"sku":"C57-6030","price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-5c584d3d0f.jpg?v=1791766977"},{"product_id":"gfp-expressing-c57bl-6-mouse-bone-marrow-macrophages","title":"GFP-Expressing C57BL\/6 Mouse Bone Marrow Macrophages","description":"GFP Expressing C57BL\/6 Mouse Bone Marrow Macrophages\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527540527388,"sku":"C57-6030GFP","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-5c584d3d0f.jpg?v=1791766977"},{"product_id":"rfp-expressing-c57bl-6-mouse-bone-marrow-macrophages","title":"RFP-Expressing C57BL\/6 Mouse Bone Marrow Macrophages","description":"RFP Expressing C57BL\/6 Mouse Bone Marrow Macrophages\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527540560156,"sku":"C57-6030RFP","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-5c584d3d0f.jpg?v=1791766977"},{"product_id":"c57bl-6-mouse-peritoneal-macrophages-thioglycollate-elicited-frozen-cells","title":"C57BL\/6 Mouse Peritoneal Macrophages - Thioglycollate-elicited (Frozen Cells)","description":"\u003cp\u003e\u003cb\u003eC57BL\/6 Mouse Peritoneal Macrophages - Thioglycollate-elicited (Frozen Cells)\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eC57-6032TF\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Macrophage Medium \/w Kit (500 ml) \u003c\/p\u003e\u003cp\u003e\u003cb\u003eCatalog No. M3368\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Peritoneal Macrophages (Thioglycollate-elicited) are isolated from the peritoneal cavity of pathogen-free laboratory adult mice. Cells at passage 0 are cryo-preserved in vials. Each vial contains 6x10\u003csup\u003e6\u003c\/sup\u003e cells and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma and tested for expression of markers using antibody, CD11b by flow cytometry. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003ePrimary Peritoneal Macrophages can not be cultured indefinitely and can not be passaged. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Peritoneal Macrophages (Thioglycollate-elicited) can be used in standard biochemical procedures include PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e will ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use. Live-cell shipment is also available on request. Primary cells can never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Peritoneal Macrophages (Thioglycollate-elicited) from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e, without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all Cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (3 x 10^6 cells)","offer_id":54527540592924,"sku":"C57-6032TF","price":580.0,"currency_code":"USD","in_stock":true},{"title":"Cells in suspension 6 x 10^6","offer_id":54687315656988,"sku":"C57-6032TF","price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-5c584d3d0f.jpg?v=1791766977"},{"product_id":"c57bl-6-mouse-bone-marrow-dendritic-cells","title":"C57BL\/6 Mouse Bone Marrow Dendritic Cells","description":"\u003cp\u003e\u003cstrong\u003eC57BL\/6 Mouse Bone Marrow Dendritic Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6200\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM7711 Complete Dendritic Cell Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Bone Marrow Dendritic Cells from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory C57BL\/6 mice. Cells at passage 0 are cryo-preserved and each vial contains 1x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. C57BL\/6 Mouse Bone Marrow Dendritic Cells are characterized by immunofluorescence staining with antibody of CD11c. These cells will not proliferate in culture and cannot be passaged.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Bone Marrow Dendritic Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e_____________________________________________________________________________________\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium:\u003c\/strong\u003e Use pre-warmed (37°C) cell culture media of Catalog No. M7711 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell recovery from cryovial:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (2 x 10^6 cells)","offer_id":54527562154268,"sku":"C57-6200","price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-b9e283e53d.jpg?v=1791767087"},{"product_id":"c57bl-6-mouse-bone-marrow-monocytes","title":"C57BL\/6 Mouse Bone Marrow Monocytes","description":"\u003cp\u003e\u003cstrong\u003eC57BL\/6 Mouse Bone Marrow Monocytes \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6271F\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM3366 Complete Leukocyte Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Bone Marrow Monocytes from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory C57BL\/6 mice. Cells at passage 0 are cryo-preserved and each vial contains 3x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. Cells in suspension culturecan be used for designed experiments under the cell culture conditions specified by Cell Biologics. Cells can be used in standard biochemical procedures include FACS, PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications. Cells are negative for bacteria, yeast, fungi, and mycoplasma. These cells will not proliferate in culture and cannot be passaged.\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Bone Marrow Monocytes from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e_____________________________________________________________________________________\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium:\u003c\/strong\u003e Use pre-warmed (37°C) cell culture media of Catalog No. M7711 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell recovery from cryovial:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003ePublication:\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/entrez\/eutils\/elink.fcgi?dbfrom=pubmed\u0026amp;retmode=ref\u0026amp;cmd=prlinks\u0026amp;id=35705056\" target=\"_blank\" rel=\"noopener\"\u003eCell Rep. 2022 Jun 14; 39(11): 110961.\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527570510108,"sku":"C57-6271","price":510.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-c3c8a4aaed.jpg?v=1791766977"},{"product_id":"c57bl-6-mouse-primary-splenocytes","title":"C57BL\/6 Mouse Primary Splenocytes","description":"\u003cp\u003e\u003cb\u003eC57BL\/6 Mouse Primary Splenocytes\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6321F\u003c\/p\u003e\u003cp\u003eSuggested Medium: Complete Cell Culture Medium\/w Kit - 500 ML\u003cbr\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. M5567\u003c\/p\u003e\u003cp\u003eProduct Description\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Primary Splenocytes are derived from the spleen tissue of pathogen-free laboratory mice. Each vial contains 20 x10\u003csup\u003e6\u003c\/sup\u003e cells and is delivered frozen. Cells in suspension culturecan be used for designed experiments under the cell culture conditions specified by Cell Biologics. Cells can be used in standard biochemical procedures include FACS, PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications. Cells are negative for bacteria, yeast, fungi, and mycoplasma. These cells will not proliferate in culture and cannot be passaged.\u003c\/p\u003e\u003cp\u003eStorage of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e will ship frozen cells on dry ice or live cells in suspension at 4 °C. On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use. Live-cell shipment is also available on request. Primary cells can never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003eAuthorized Uses of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Primary Splenocytes from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for \u003ci\u003ein vitro\u003c\/i\u003e diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics,\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003eDisclaimer\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all Cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003eWarranty and Liability\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (10 x 10^6 cells)","offer_id":54527571067164,"sku":"C57-6321","price":510.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-34b0a0790d.jpg?v=1791766978"},{"product_id":"cd1-mouse-bone-marrow-neutrophils","title":"CD1 Mouse Bone Marrow Neutrophils","description":"\u003cp\u003e\u003cstrong\u003eCD1 Mouse Bone Marrow Neutrophils \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. CD-1029F\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM3367 Complete Neutrophil Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCD1 Mouse Bone Marrow Neutrophils from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory CD1 mice. The cells at passage 0 are cryo-preserved, each vial contains 5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. These cells can be used for designed experiments under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCD1 Mouse Bone Marrow Neutrophils from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e_______________________________________________________________________________________\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003eMedium: Use pre-warmed (37°C) cell culture media of Catalog No. M3367 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003eCell recovery from cryovial:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527573623068,"sku":"CD-1029","price":510.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-7c89b4d443.jpg?v=1791766977"},{"product_id":"cd1-mouse-bone-marrow-macrophages-frozen-cells","title":"CD1 Mouse Bone Marrow Macrophages (Frozen Cells)","description":"\u003cp\u003e\u003cb\u003eCD1 Mouse Bone Marrow Macrophages (Frozen Cells)\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eCD-1030\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Macrophage Medium \/w Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCatalog No. M3368\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCD1 Mouse Bone Marrow Macrophages are isolated from the tibias and femurs of pathogen-free laboratory adult mice. Cells at passage 0 are cryo-preserved in vials. Each vial contains 3x10\u003csup\u003e6\u003c\/sup\u003e cells and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma and tested for expression of markers using antibody, CD11b by flow cytometry. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eCells can be plated on culture plates for desired experiments under the cell culture conditions specified by Cell Biologics. Primary Bone Marrow Macrophages can not be cultured indefinitely and can not be passaged. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCD1 Mouse Bone Marrow Macrophages can be used in standard biochemical procedures include PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e will ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use. Live-cell shipment is also available on request. Primary cells can never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCD1 Mouse Bone Marrow Macrophages from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for \u003ci\u003ein vitro\u003c\/i\u003e diagnostic procedures or for therapeutic procedures. 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