{"title":"Monkey Hepatocytes, Stellate Cells, Kupffer Cells","description":"\u003cp\u003eCustom Cell Isolation (Customer will need to provide\/purchase tissues for cell isolation).\u003c\/p\u003e","products":[{"product_id":"cynomolgus-monkey-liver-kupffer-cells","title":"Cynomolgus Monkey Liver Kupffer Cells","description":"\u003cp\u003e\u003cstrong\u003eMonkey Primary Liver Kupffer Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. MK-6226S\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM6030 Complete Macrophage Medium \/w Kit (125 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eMonkey Primary Liver Kupffer Cells from Cell Biologics are isolated from Cynomolgus Monkey liver tissues and grown in tissue culture plates with Cell Biologics’ Cell Culture Medium. Live Cells at passage 0 in culture plates or flasks are shipped and cells are ready used for experiments. Cells are characterized by immunofluorescence staining using an antibody of CD11b. Primary Liver Kupffer Cells can not be cultured indefinitely and can not be passaged.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eHandling of Arriving Live Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLive Cells at passage 0 in culture plates or flasks are shipped overnight. When you receive the live cells in plates or flasks, remove the sticker from the filter cap, and keep the plates or flasks with 3-15 ml existing medium in 37°C CO\u003csub\u003e2\u003c\/sub\u003e incubator for 1 hour before replacing the desired Cell Biologics' cell culture medium.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eMonkey Primary Liver Kupffer Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e__________________________________________________________________________________\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Liver Kupffer Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a bio-safety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eReview the information provided on the Cell Biologics website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture media (30-50 ML, Catalog No. M6030) to seed cells and when changing media.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e\u003cu\u003eHandling of Arriving Live Cells\u003c\/u\u003e\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLive Cells at passage 0 in a culture plate or flask are shipped overnight. When you receive the live cells in a plate or flask, keep the plate or flask with suggested medium volumn in 37°C CO\u003csup\u003e2\u003c\/sup\u003e incubator for 1 hour before replacing the desired Cell Biologics' cell culture medium.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eNote: A recommended media volume for culture dishes, plates and flasks:\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e6-well plate: Use 2 ml medium per well;\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e12-well plate: Use 0.5 ml medium per well;\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e96-well: Use 100 ul medium per well;\u003cbr\u003e\u003c\/p\u003e\u003cp\u003eCells in T25 flask; Use 5ml medium;\u003cbr\u003e\u003c\/p\u003e\u003cp\u003eCells in T75 flask: Use 10 ml medium\u003c\/p\u003e\u003cp\u003eProceed with experiment assays.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"6 wells","offer_id":54527771771164,"sku":"MK-6226S","price":1200.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687883755804,"sku":"MK-6226S","price":1200.0,"currency_code":"USD","in_stock":true},{"title":"12 wells","offer_id":54687883788572,"sku":"MK-6226S","price":1200.0,"currency_code":"USD","in_stock":true},{"title":"24 wells","offer_id":54687883821340,"sku":"MK-6226S","price":1200.0,"currency_code":"USD","in_stock":true},{"title":"96 wells","offer_id":54687883854108,"sku":"MK-6226S","price":1200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-63352c930e.jpg?v=1791766977"}],"url":"https:\/\/jkpgye-36.myshopify.com\/collections\/monkey-hepatocytes-stellate-cells-kupffer-cells.oembed","provider":"Cell Biologics","version":"1.0","type":"link"}