{"title":"Human Tumor Cells","description":"\u003cp\u003e\u003cstrong\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e \u003c\/strong\u003eprovides custom cell isolation service to our customers upon request. On request, we will isolate cells of any description from specific organs or tissues (any animal or human tissues) designated by our customers.\u003c\/p\u003e\u003cp\u003eIf you would like to use of Custom Cell Isolation Service, for details regarding these special services, please contact us at \u003cstrong\u003eservice@cellbiologics.com.\u003c\/strong\u003e\u003c\/p\u003e","products":[{"product_id":"human-colon-tumor-associated-smooth-muscle-cells","title":"Human Colon Tumor-Associated Smooth Muscle Cells","description":"\u003cp\u003eFor Research Use Only\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eHuman Primary Colonic Tumor-Associated Smooth Muscle Cells \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. HC-6001\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2268 Complete Smooth Muscle Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Colonic Tumor-Associated Smooth Muscle Cells from Cell Biologics are isolated from human colonic tumor tissues and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Human Primary Colonic Tumor-Associated Smooth Muscle Cells from Cell Biologics are characterized by immunofluorescence staining with antibody of α-smooth muscle actin. These cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Colonic Tumor-Associated Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527697879324,"sku":"HC-6001","price":850.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-54bed8ead2.jpg?v=1791767087"},{"product_id":"human-lung-tumor-associated-endothelial-cells-adenocarcinoma","title":"Human Lung Tumor-Associated Endothelial Cells (Adenocarcinoma)","description":"\u003cp\u003e\u003cb\u003eHuman Lung Tumor-Associated Endothelial Cells (Adenocarcinoma)\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eHC-6011A\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Complete Human Endothelial Cell Medium– 500ML\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eH1168\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor-Associated Endothelial Cells (Adenocarcinoma) from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from human lung adenocarcinoma tissue. Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor-Associated Endothelial Cells (Adenocarcinoma) from \u003ci\u003eCell Biologics\u003c\/i\u003e display typical cobblestone with large dark nuclei appearance under light microscopy. Cells are tested for expression of endothelial cell marker using antibody, CD31 (Catalog No. 550389, BD; CD31\/PECAM-1 PE-conjugated Antibody, Catalog No. FAB3567P, R\u0026amp;D) or VE-Cadherin (FITC-VE-cadherin Catalog No. 560411, BD) by immunofluorescence staining or FACS. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and\/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased. Cells can be expanded for 2-4 passages under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor-Associated Endothelial Cells (Adenocarcinoma) can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor-Associated Endothelial Cells (Adenocarcinoma) from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for \u003ci\u003ein vitro\u003c\/i\u003e diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics,\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e human endothelial cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution, for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’ \u003c\/i\u003eonline protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527698043164,"sku":"HC-6011A","price":1012.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-e7ccefa31b.jpg?v=1791766977"},{"product_id":"human-lung-tumor-associated-endothelial-cells-squamous-cell-carcinoma","title":"Human Lung Tumor-Associated Endothelial Cells ( Squamous Cell Carcinoma)","description":"\u003cp\u003e\u003cb\u003eHuman Lung Tumor-Associated Endothelial Cells (Squamous Cell Carcinoma) \u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eHC-6011S\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Complete Human Endothelial Cell Medium– 500ML\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eH1168\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor-Associated Endothelial Cells (Squamous Cell Carcinoma) from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from human lung squamous cell carcinoma tissue. Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor-Associated Endothelial Cells (Squamous Cell Carcinoma) from \u003ci\u003eCell Biologics\u003c\/i\u003e display typical cobblestone with large dark nuclei appearance under light microscopy. Cells are tested for expression of endothelial cell marker using antibody, CD31 (Catalog No. 550389, BD; CD31\/PECAM-1 PE-conjugated Antibody, Catalog No. FAB3567P, R\u0026amp;D) or VE-Cadherin (FITC-VE-cadherin Catalog No. 560411, BD) by immunofluorescence staining or FACS. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and\/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased. Cells can be expanded for 2-4 passages under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor-Associated Endothelial Cells (Squamous Cell Carcinoma) can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor-Associated Endothelial Cells (Squamous Cell Carcinoma) from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics,\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e human endothelial cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution， for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’ \u003c\/i\u003eonline protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527698075932,"sku":"HC-6011S","price":1012.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-35de6c69af.jpg?v=1791767016"},{"product_id":"human-lung-tumor-associated-fibroblasts-adenocarcinoma","title":"Human Lung Tumor-Associated Fibroblasts - Adenocarcinoma","description":"\u003cp\u003e\u003cb\u003eHuman Lung Tumor-Associated Fibroblasts - Adenocarcinoma\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eHC-6013A\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Fibroblast Medium \/w Kit –500 ml\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eM2267\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor-Associated Fibroblasts from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from human lung Adenocarcinoma tissue. Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor-Associated Fibroblasts from \u003ci\u003eCell Biologics\u003c\/i\u003e are tested for negative expression of von Willebrand Factor Expression\/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor-Associated Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should be never kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics\u003c\/i\u003e’ Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor-Associated Fibroblasts from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics, \u003c\/i\u003ewithout the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution, for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527698108700,"sku":"HC-6013A","price":850.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687843582236,"sku":"HC-6013A","price":912.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687843615004,"sku":"HC-6013A","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-16c0aba89f.jpg?v=1791767054"},{"product_id":"human-lung-tumor-associated-fibroblasts-squamous-cell-carcinoma","title":"Human Lung Tumor-Associated Fibroblasts - Squamous Cell Carcinoma","description":"\u003cp\u003e\u003cb\u003eHuman Lung Tumor-Associated Fibroblasts - Squamous Cell Carcinoma \u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eHC-6013S\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Fibroblast Medium \/w Kit –500 ml\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eM2267\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor-Associated Fibroblasts from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from human lung squamous cell carcinoma tissue. Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor-Associated Fibroblasts from \u003ci\u003eCell Biologics\u003c\/i\u003e are tested for negative expression of von Willebrand Factor Expression\/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor-Associated Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should be never kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics\u003c\/i\u003e’ Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor-Associated Fibroblasts from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics, \u003c\/i\u003ewithout the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. 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Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Human Primary Liver Tumor-Associated Fibroblasts are characterized by their spindle morphology and immunofluorescent staining with anti-fibroblast-specific protein 1 or anti-Vimentin antibody. These cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. 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Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Human Primary Ovarian Tumor-Associated Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Ovarian Tumor-Associated Epithelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. 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Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Human Primary Prostate Tumor-Associated Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Prostate Tumor-Associated Epithelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527698829596,"sku":"HC-6038","price":850.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687845024028,"sku":"HC-6038","price":912.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687845056796,"sku":"HC-6038","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-69b2230c2d.jpg?v=1791767054"},{"product_id":"human-prostate-tumor-associated-smooth-muscle-cells","title":"Human Prostate Tumor-Associated Smooth Muscle Cells","description":"\u003cp\u003e\u003cstrong\u003eHuman Primary Prostate Tumor-Associated Smooth Muscle Cells \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. HC-6041\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2268 Complete Smooth Muscle Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Prostate Tumor-Associated Smooth Muscle Cells from Cell Biologics are isolated from human prostate tumor tissues and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Human Primary Prostate Tumor-Associated Smooth Muscle Cells from Cell Biologics are characterized by immunofluorescence staining with antibody of α-smooth muscle actin. These cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Prostate Tumor-Associated Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527698862364,"sku":"HC-6041","price":850.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-54bed8ead2.jpg?v=1791767087"},{"product_id":"human-liver-tumor-epithelial-cells","title":"Human Liver Tumor Epithelial Cells","description":"\u003cp\u003e\u003cb\u003eHuman Liver Tumor Epithelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eHC-60\u003c\/b\u003e\u003cb\u003e44\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Complete Human Epithelial Cell Medium \/w Kit\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eH6621\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Liver Tumor Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from human liver tumor tissue. Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Liver Tumor Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are tested for expression of markers using antibody, E-cadherin (Catalog No. 610182 BD) or ZO-1 Rabbit Polyclonal Antibody (Catalog No. 617300; Life Technologies) by immunofluorescence staining. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and\/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Liver Tumor Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics\u003c\/i\u003e’ Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells can never be kept at -20°C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics\u003c\/i\u003e’ Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Liver Tumor Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for \u003ci\u003ein vitro\u003c\/i\u003e diagnostic procedures or not for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics, \u003c\/i\u003ewithout the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution, for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527698895132,"sku":"HC-6044","price":850.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687845122332,"sku":"HC-6044","price":850.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687845155100,"sku":"HC-6044","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-69b2230c2d.jpg?v=1791767054"},{"product_id":"human-colon-tumor-epithelial-cells","title":"Human Colon Tumor Epithelial Cells","description":"\u003cp\u003e\u003cstrong\u003eHuman Primary Colonic Tumor-Associated Epithelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. HC-6047\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Colonic Tumor-Associated Epithelial Cells from Cell Biologics are isolated from human colonic tumor tissues and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Human Primary Colonic Tumor-Associated Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary colonic Tumor-Associated Epithelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527698927900,"sku":"HC-6047","price":850.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687845187868,"sku":"HC-6047","price":912.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687845220636,"sku":"HC-6047","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-770f4123f9.jpg?v=1791767016"},{"product_id":"human-lung-tumor-epithelial-cells-adenocarcinoma","title":"Human Lung Tumor Epithelial Cells - Adenocarcinoma","description":"\u003cp\u003e\u003cb\u003eHuman Lung Tumor\u003c\/b\u003e\u003cb\u003eEpithelial Cells -\u003c\/b\u003e\u003cb\u003eAdenocarcinoma\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eHC-60\u003c\/b\u003e\u003cb\u003e53A\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Complete Human Epithelial Cell Medium \/w Kit\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eH6621\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from human lung adenocarcinoma tissue. Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are tested for expression of markers using antibody, E-cadherin (Catalog No. 610182 BD) or ZO-1 Rabbit Polyclonal Antibody (Catalog No. 617300; Life Technologies) by immunofluorescence staining. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and\/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics\u003c\/i\u003e’ Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells can never be kept at -20°C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics\u003c\/i\u003e’ Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for \u003ci\u003ein vitro\u003c\/i\u003e diagnostic procedures or not for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics, \u003c\/i\u003ewithout the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution, for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527698993436,"sku":"HC-6053A","price":850.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687845286172,"sku":"HC-6053A","price":912.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687845318940,"sku":"HC-6053A","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-f70a5d6bad.jpg?v=1791767017"},{"product_id":"human-lung-tumor-epithelial-cells-squamous-cell-carcinoma","title":"Human Lung Tumor Epithelial Cells - Squamous Cell Carcinoma","description":"\u003cp\u003e\u003cb\u003eHuman Lung\u003c\/b\u003e\u003cb\u003e Tumor Epithelial Cells -\u003c\/b\u003e\u003cb\u003eSquamous Cell Carcinoma\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eHC-60\u003c\/b\u003e\u003cb\u003e53S\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Complete Human Epithelial Cell Medium \/w Kit\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eH6621\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from human lung squamous cell carcinoma tissue. Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are tested for expression of markers using antibody, E-cadherin (Catalog No. 610182 BD) or ZO-1 Rabbit Polyclonal Antibody (Catalog No. 617300; Life Technologies) by immunofluorescence staining. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and\/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics\u003c\/i\u003e’ Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells can never be kept at -20°C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics\u003c\/i\u003e’ Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Lung Tumor Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for \u003ci\u003ein vitro\u003c\/i\u003e diagnostic procedures or not for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics, \u003c\/i\u003ewithout the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution, for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527699124508,"sku":"HC-6053S","price":850.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687845351708,"sku":"HC-6053S","price":912.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687845384476,"sku":"HC-6053S","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-770f4123f9.jpg?v=1791767016"},{"product_id":"human-melanoma-tumor-associated-endothelial-cells","title":"Human Melanoma Tumor-Associated Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eHuman Primary Dermal Microvascular Tumor-Associated Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. HC-6064\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Dermal Microvascular Tumor-Associated Endothelial Cells from Cell Biologics are isolated from human skin tumor tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Human Primary Dermal Microvascular Tumor-Associated Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for Tumor-Associated Endothelial Cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Dermal Microvascular Tumor-Associated Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. 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Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Human Primary Dermal Tumor-Associated Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. 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Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. 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Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Human Primary Ovarian Tumor-Associated Fibroblasts are characterized by their spindle morphology and immunofluorescent staining with anti-fibroblast-specific protein 1 or anti-Vimentin antibody. These cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. 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Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. 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Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Bronchial Tumor-Associated Fibroblasts from \u003ci\u003eCell Biologics\u003c\/i\u003e are tested for negative expression of von Willebrand Factor Expression\/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Bronchial Tumor-Associated Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should be never kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics\u003c\/i\u003e’ Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Bronchial Tumor-Associated Fibroblasts from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics, \u003c\/i\u003ewithout the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution, for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003eonline protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527699681564,"sku":"HC-6217B","price":737.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687845712156,"sku":"HC-6217B","price":800.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687845744924,"sku":"HC-6217B","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-16c0aba89f.jpg?v=1791767054"},{"product_id":"human-prostate-tumor-associated-fibroblasts","title":"Human Prostate Tumor-Associated Fibroblasts","description":"\u003cp\u003e\u003cstrong\u003eHuman Primary Prostate Tumor-Associated Fibroblasts\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. HC-6223\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2267 Complete Fibroblast Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Prostate Tumor-Associated Fibroblasts from Cell Biologics are isolated from human prostate tumor tissues and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. \u003c\/p\u003e\u003cp\u003eHuman Primary Prostate Tumor-Associated Fibroblasts are characterized by their spindle morphology and immunofluorescent staining with anti-fibroblast-specific protein 1 or anti-Vimentin antibody. These cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Prostate Tumor-Associated Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527699714332,"sku":"HC-6223","price":737.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687845777692,"sku":"HC-6223","price":800.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687845810460,"sku":"HC-6223","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-16c0aba89f.jpg?v=1791767054"},{"product_id":"human-colon-associated-tumor-fibroblasts","title":"Human Colon-Associated Tumor Fibroblasts","description":"\u003cp\u003e\u003cstrong\u003eHuman Primary Colonic Tumor-Associated Fibroblasts \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. HC-6231\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2267 Complete Fibroblast Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Colonic Tumor-Associated Fibroblasts from Cell Biologics are isolated from human colonic tumor tissues and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. \u003c\/p\u003e\u003cp\u003eHuman Primary Colonic Tumor-Associated Fibroblasts are characterized by their spindle morphology and immunofluorescent staining with anti-fibroblast-specific protein 1 or anti-Vimentin antibody. These cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Colonic Tumor-Associated Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527699845404,"sku":"HC-6231","price":737.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687845875996,"sku":"HC-6231","price":800.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687845908764,"sku":"HC-6231","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-16c0aba89f.jpg?v=1791767054"},{"product_id":"human-ovarian-metastatic-tumor-associated-endothelial-cells","title":"Human Ovarian Metastatic Tumor -Associated Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eHuman Ovarian Metastatic Tumor-Associated Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eHC-6263\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Complete Human Endothelial Cell Medium– 500ML\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eH1168\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Ovarian Metastatic Tumor-Associated Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from human metastatic ovarian tumor tissue developed from colon cancer. Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Ovarian Metastatic Tumor-Associated Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e display typical cobblestone with large dark nuclei appearance under light microscopy. Cells are tested for expression of endothelial cell marker using antibody, CD31 (Catalog No. 550389, BD; CD31\/PECAM-1 PE-conjugated Antibody, Catalog No. FAB3567P, R\u0026amp;D) or VE-Cadherin (FITC-VE-cadherin Catalog No. 560411, BD) by immunofluorescence staining or FACS. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and\/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased. Cells can be expanded for 2-4 passages under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Ovarian Metastatic Tumor-Associated Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Ovarian Metastatic Tumor-Associated Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics,\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Although Human Ovarian Tumor-Associated Endothelial Cells are isolated from normal human tissue, investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e human endothelial cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution, for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’ \u003c\/i\u003eonline protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527699878172,"sku":"HC-6263","price":1012.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-2aa43629cf.jpg?v=1791767054"},{"product_id":"human-ovarian-metastatic-tumor-epithelial-cells","title":"Human Ovarian Metastatic Tumor Epithelial Cells","description":"\u003cp\u003e\u003cb\u003eHuman Ovarian Metastatic Tumor Epithelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eHC-6264\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Complete Human Epithelial Cell Medium \/w Kit\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eH6621\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Ovarian Metastatic Tumor Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from human metastatic ovarian tissue developed from colon cancer. Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Ovarian Metastatic Tumor Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are tested for expression of markers using antibody, E-cadherin (Catalog No. 610182 BD) or ZO-1 Rabbit Polyclonal Antibody (Catalog No. 617300; Life Technologies) by immunofluorescence staining. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and\/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Ovarian Metastatic Tumor Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics\u003c\/i\u003e’ Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics\u003c\/i\u003e’ Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Ovarian Metastatic Tumor Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for \u003ci\u003ein vitro\u003c\/i\u003e diagnostic procedures or not for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics, \u003c\/i\u003ewithout the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution, for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527699910940,"sku":"HC-6264","price":850.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687845974300,"sku":"HC-6264","price":912.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687846007068,"sku":"HC-6264","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-7226305f2e.jpg?v=1791767054"},{"product_id":"human-metastatic-ovarian-tumor-associated-fibroblasts","title":"Human Metastatic Ovarian Tumor-Associated Fibroblasts","description":"\u003cp\u003e\u003cb\u003eHuman \u003c\/b\u003e\u003cb\u003eMetastatic Ovarian \u003c\/b\u003e\u003cb\u003eTumor-Associated Fibroblasts\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eHC-6265\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Fibroblast Medium \/w Kit –500 ml\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eM2267\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Metastatic Ovarian Tumor-Associated Fibroblasts from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from human metastatic ovarian tissue developed from colon cancer. Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Metastatic Ovarian Tumor-Associated Fibroblasts from \u003ci\u003eCell Biologics\u003c\/i\u003e are tested for negative expression of von Willebrand Factor Expression\/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Metastatic Ovarian Tumor-Associated Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should be never kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics\u003c\/i\u003e’ Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Metastatic Ovarian Tumor-Associated Fibroblasts from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics, \u003c\/i\u003ewithout the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution, for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527699943708,"sku":"HC-6265","price":737.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687846039836,"sku":"HC-6265","price":800.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687846072604,"sku":"HC-6265","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-2821381262.jpg?v=1791767054"},{"product_id":"human-ovarian-metastatic-tumor-associated-smooth-muscle-cells","title":"Human Ovarian Metastatic Tumor -Associated Smooth Muscle Cells","description":"\u003cp\u003e\u003cb\u003eHuman Ovarian Metastatic Tumor-Associated Smooth Muscle Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eHC-6\u003c\/b\u003e\u003cb\u003e266\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Smooth Muscle Cell Medium \/w Kit –500 ML\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eM2268\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Ovarian Metastatic Tumor-Associated Smooth Muscle Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from human metastatic ovarian tissue developed from colon cancer. Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Prior to shipping, cells at passage 1 are detached from the culture flasks and immediately cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Cells are characterized by immunofluorescent method with antibodies to α-smooth muscle actin. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments (or cells may be expanded for 2-5 passages at a split ratio of 1:2) under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Ovarian Metastatic Tumor-Associated Smooth Muscle Cells can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request. Primary cells can never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Ovarian Metastatic Tumor-Associated Smooth Muscle Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures, or not for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e, without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003eonline protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527699976476,"sku":"HC-6266","price":762.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-54bed8ead2.jpg?v=1791767087"}],"url":"https:\/\/jkpgye-36.myshopify.com\/collections\/human-tumor-cells.oembed","provider":"Cell Biologics","version":"1.0","type":"link"}