{"title":"Human Diabetic Endothelial Cells - Type II","description":"\u003cp\u003eHuman Diabetic Endothelial Cells - Type II from Cell Biologics, isolated and quality-tested in our ISO 9001 laboratory for research use.\u003c\/p\u003e","products":[{"product_id":"human-primary-diabetic-artery-endothelial-cells","title":"Human Primary Diabetic Artery Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eHuman Diabetic Primary Artery Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. HD2-6008\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Artery Endothelial Cells from Cell Biologics are isolated from the artery tissue of human donor that has been diagnosed with diabetes type II disease. These cells are grown in gelatin pre-coated tissue culture flasks with Cell Biologics’ Complete Growth Medium and harvested from flasks at passage 3. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. Human Diabetic Primary Artery Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Artery Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003c\/p\u003e\u003cp\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527700009244,"sku":"HD2-6008","price":887.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687846105372,"sku":"HD2-6008","price":950.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687846138140,"sku":"HD2-6008","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-a939a886c7.jpg?v=1791767016"},{"product_id":"human-primary-diabetic-vein-endothelial-cells","title":"Human Primary Diabetic Vein Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eHuman Diabetic Primary Vein Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. HD2-6009\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Vein Endothelial Cells from Cell Biologics are isolated from human vein tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Human Diabetic Primary Vein Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for Endothelial Cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Vein Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003c\/p\u003e\u003cp\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527700042012,"sku":"HD2-6009","price":887.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687846170908,"sku":"HD2-6009","price":950.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687846203676,"sku":"HD2-6009","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-a939a886c7.jpg?v=1791767016"},{"product_id":"human-primary-diabetic-lung-microvascular-endothelial-cells","title":"Human Primary Diabetic Lung Microvascular Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eHuman Diabetic Primary Lung Microvascular Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. HD2-6011\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Lung Microvascular Endothelial Cells from Cell Biologics are isolated from human lung tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Human Diabetic Primary Lung Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for Endothelial Cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Lung Microvascular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003c\/p\u003e\u003cp\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527700173084,"sku":"HD2-6011","price":887.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687846301980,"sku":"HD2-6011","price":950.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687846334748,"sku":"HD2-6011","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-a939a886c7.jpg?v=1791767016"},{"product_id":"human-primary-diabetic-kidney-endothelial-cells","title":"Human Primary Diabetic Kidney Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eHuman Diabetic Primary Kidney Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. HD2-6014\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Kidney Endothelial Cells from Cell Biologics are isolated from human kidney tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Human Diabetic Primary Kidney Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for Endothelial Cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Kidney Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003c\/p\u003e\u003cp\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527700238620,"sku":"HD2-6014","price":887.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687846433052,"sku":"HD2-6014","price":950.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687846465820,"sku":"HD2-6014","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-a939a886c7.jpg?v=1791767016"},{"product_id":"human-primary-diabetic-kidney-artery-endothelial-cells","title":"Human Primary Diabetic Kidney Artery Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eHuman Primary Diabetic Kidney Artery \u003c\/b\u003e\u003cb\u003eEndothelial Cells (HDKAEC)\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eHD2-6014A\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=h1168\"\u003eComplete Human Endothelial Cell Medium– 500 ML\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003e\u003ca href=\"\/search?q=h1168\"\u003eH1168\u003c\/a\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eProduct Description\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Diabetic Kidney Artery Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from the kidney artery of human donor that has been diagnosed with diabetes type II disease. Human Primary Diabetic Kidney Artery Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, Cells at passage 3 are detached from flasks and immediately cryopreserved in vails. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. \u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eProduct Testing\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Diabetic Kidney Artery Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e display typical cobblestone with large dark nuclei appearance under light microscopy. Cells are tested for expression of endothelial cell marker using antibody, CD31 (Catalog No. 550389, BD; CD31\/PECAM-1 PE-conjugated Antibody, Catalog No. FAB3567P, R\u0026amp;D) or VE-Cadherin (FITC-VE-cadherin Catalog No. 560411, BD) by immunofluorescence staining or FACS. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and\/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased. Cells can be expanded for 3-5 passages under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eLaboratory Applications\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Diabetic Kidney Artery Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Diabetic Kidney Artery Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eDisclaimer\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003ca href=\"http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm\" target=\"_blank\" rel=\"noopener\"\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eWarranty and Liability\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003cem\u003eCell Biologics’\u003c\/em\u003e human endothelial cells with \u003cem\u003eCell Biologics’\u003c\/em\u003e Media (catalog number H1168)and Coating Solution for appropriate endothelial cell culture and cell testing following \u003cem\u003eCell Biologics’\u003c\/em\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527700271388,"sku":"HD2-6014A","price":887.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687846498588,"sku":"HD2-6014A","price":950.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687846531356,"sku":"HD2-6014A","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-a939a886c7.jpg?v=1791767016"},{"product_id":"human-primary-diabetic-kidney-vein-endothelial-cells","title":"Human Primary Diabetic Kidney Vein Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eHuman Primary Diabetic Kidney Artery \u003c\/b\u003e\u003cb\u003eEndothelial Cells (HDKV\u003c\/b\u003e\u003cb\u003eEC)\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eHD2-6014V\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=h1168\"\u003eComplete Human Endothelial Cell Medium– 500 ML\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003e\u003ca href=\"\/search?q=h1168\"\u003eH1168\u003c\/a\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eProduct Description\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Diabetic Kidney Vein Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from the kidney vein of human donor that has been diagnosed with diabetes type II disease. Human Primary Diabetic Kidney Vein Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, Cells at passage 3 are detached from flasks and immediately cryopreserved in vails. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. \u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eProduct Testing\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Diabetic Kidney Vein Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e display typical cobblestone with large dark nuclei appearance under light microscopy. Cells are tested for expression of endothelial cell marker using antibody, CD31 (Catalog No. 550389, BD; CD31\/PECAM-1 PE-conjugated Antibody, Catalog No. FAB3567P, R\u0026amp;D) or VE-Cadherin (FITC-VE-cadherin Catalog No. 560411, BD) by immunofluorescence staining or FACS. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and\/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased. Cells can be expanded for 3-5 passages under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eLaboratory Applications\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Diabetic Kidney Vein Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Diabetic Kidney Vein Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eDisclaimer\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003ca href=\"http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm\" target=\"_blank\" rel=\"noopener\"\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eWarranty and Liability\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003cem\u003eCell Biologics’\u003c\/em\u003e human endothelial cells with \u003cem\u003eCell Biologics’\u003c\/em\u003e Media (catalog number H1168)and Coating Solution for appropriate endothelial cell culture and cell testing following \u003cem\u003eCell Biologics’\u003c\/em\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527700304156,"sku":"HD2-6014V","price":887.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687846596892,"sku":"HD2-6014V","price":950.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687846629660,"sku":"HD2-6014V","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-a939a886c7.jpg?v=1791767016"},{"product_id":"human-primary-diabetic-liver-endothelial-cells","title":"Human Primary Diabetic Liver Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eHuman Diabetic Primary Liver Sinusoidal Microvascular Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. HD2-6017\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Liver Sinusoidal Microvascular Endothelial Cells from Cell Biologics are isolated from human liver tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Human Diabetic Primary Liver Sinusoidal Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for Endothelial Cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Liver Sinusoidal Microvascular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003c\/p\u003e\u003cp\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527700533532,"sku":"HD2-6017","price":887.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687846891804,"sku":"HD2-6017","price":950.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687846924572,"sku":"HD2-6017","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-a939a886c7.jpg?v=1791767016"},{"product_id":"human-primary-diabetic-liver-artery-endothelial-cells","title":"Human Primary Diabetic Liver Artery Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eHuman Primary Diabetic Liver Artery \u003c\/b\u003e\u003cb\u003eEndothelial Cells (HDL\u003c\/b\u003e\u003cb\u003eAEC)\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eHD2-6017A\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=h1168\"\u003eComplete Human Endothelial Cell Medium– 500 ML\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003e\u003ca href=\"\/search?q=h1168\"\u003eH1168\u003c\/a\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eProduct Description\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Diabetic Liver Artery Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from the liver artery of human donor that has been diagnosed with diabetes type II disease. Human Primary Diabetic Liver Artery Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, Cells at passage 3 are detached from flasks and immediately cryopreserved in vails. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. \u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eProduct Testing\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Diabetic Liver Artery Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e display typical cobblestone with large dark nuclei appearance under light microscopy. Cells are tested for expression of endothelial cell marker using antibody, CD31 (Catalog No. 550389, BD; CD31\/PECAM-1 PE-conjugated Antibody, Catalog No. FAB3567P, R\u0026amp;D) or VE-Cadherin (FITC-VE-cadherin Catalog No. 560411, BD) by immunofluorescence staining or FACS. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and\/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased. Cells can be expanded for 3-5 passages under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eLaboratory Applications\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Diabetic Liver Artery Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Diabetic Liver Artery Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eDisclaimer\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003ca href=\"http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm\" target=\"_blank\" rel=\"noopener\"\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eWarranty and Liability\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003cem\u003eCell Biologics’\u003c\/em\u003e human endothelial cells with \u003cem\u003eCell Biologics’\u003c\/em\u003e Media (catalog number H1168)and Coating Solution for appropriate endothelial cell culture and cell testing following \u003cem\u003eCell Biologics’\u003c\/em\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527700566300,"sku":"HD2-6017A","price":887.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687846957340,"sku":"HD2-6017A","price":950.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687846990108,"sku":"HD2-6017A","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-a939a886c7.jpg?v=1791767016"},{"product_id":"human-primary-diabetic-liver-vein-endothelial-cells","title":"Human Primary Diabetic Liver Vein Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eHuman Primary Diabetic Liver Vein \u003c\/b\u003e\u003cb\u003eEndothelial Cells (HDL\u003c\/b\u003e\u003cb\u003eVEC)\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eHD2-6017V\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=h1168\"\u003eComplete Human Endothelial Cell Medium– 500 ML\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003e\u003ca href=\"\/search?q=h1168\"\u003eH1168\u003c\/a\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eProduct Description\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Diabetic Liver Vien Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from the liver vein of human donor that has been diagnosed with diabetes type II disease. Human Primary Diabetic Liver Vien Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, Cells at passage 3 are detached from flasks and immediately cryopreserved in vails. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. \u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eProduct Testing\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Diabetic Liver Vien Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e display typical cobblestone with large dark nuclei appearance under light microscopy. Cells are tested for expression of endothelial cell marker using antibody, CD31 (Catalog No. 550389, BD; CD31\/PECAM-1 PE-conjugated Antibody, Catalog No. FAB3567P, R\u0026amp;D) or VE-Cadherin (FITC-VE-cadherin Catalog No. 560411, BD) by immunofluorescence staining or FACS. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and\/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased. Cells can be expanded for 3-5 passages under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eLaboratory Applications\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Diabetic Liver Vien Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Diabetic Liver Vien Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eDisclaimer\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003ca href=\"http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm\" target=\"_blank\" rel=\"noopener\"\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eWarranty and Liability\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003cem\u003eCell Biologics’\u003c\/em\u003e human endothelial cells with \u003cem\u003eCell Biologics’\u003c\/em\u003e Media (catalog number H1168)and Coating Solution for appropriate endothelial cell culture and cell testing following \u003cem\u003eCell Biologics’\u003c\/em\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527700599068,"sku":"HD2-6017V","price":887.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687847022876,"sku":"HD2-6017V","price":950.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687847055644,"sku":"HD2-6017V","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-a939a886c7.jpg?v=1791767016"},{"product_id":"human-primary-diabetic-cardiac-endothelial-cells","title":"Human Primary Diabetic Cardiac Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eHuman Diabetic Primary Cardiac Microvascular Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. HD2-6024\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Cardiac Microvascular Endothelial Cells from Cell Biologics are isolated from human heart tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Human Diabetic Primary Cardiac Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for Endothelial Cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Cardiac Microvascular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003c\/p\u003e\u003cp\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527700730140,"sku":"HD2-6024","price":887.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687847317788,"sku":"HD2-6024","price":950.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687847350556,"sku":"HD2-6024","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-a939a886c7.jpg?v=1791767016"},{"product_id":"human-primary-diabetic-aortic-endothelial-cells","title":"Human Primary Diabetic Aortic Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eHuman Diabetic Primary Aortic Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. HD2-6052\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Aortic Endothelial Cells from Cell Biologics are isolated from human aorta tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Human Diabetic Primary Aortic Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for Endothelial Cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Aortic Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003c\/p\u003e\u003cp\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527701090588,"sku":"HD2-6052","price":887.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687847842076,"sku":"HD2-6052","price":950.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687847874844,"sku":"HD2-6052","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-a939a886c7.jpg?v=1791767016"},{"product_id":"human-primary-diabetic-pulmonary-artery-endothelial-cells","title":"Human Primary Diabetic Pulmonary Artery Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eHuman Diabetic Primary Pulmonary Artery Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. HD2-6059\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Pulmonary Artery Endothelial Cells from Cell Biologics are isolated from human pulmonary artery tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Human Diabetic Primary Pulmonary Artery Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for Endothelial Cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Pulmonary Artery Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003c\/p\u003e\u003cp\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527701221660,"sku":"HD2-6059","price":887.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687847973148,"sku":"HD2-6059","price":950.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687848005916,"sku":"HD2-6059","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-a939a886c7.jpg?v=1791767016"},{"product_id":"human-primary-diabetic-pulmonary-vein-endothelial-cells","title":"Human Primary Diabetic Pulmonary Vein Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eHuman Diabetic Primary Pulmonary Vein Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. HD2-6060\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Pulmonary Vein Endothelial Cells from Cell Biologics are isolated from human pulmonary vein tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Human Diabetic Primary Pulmonary Vein Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for Endothelial Cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Pulmonary Vein Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003c\/p\u003e\u003cp\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527701352732,"sku":"HD2-6060","price":887.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687848038684,"sku":"HD2-6060","price":950.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687848071452,"sku":"HD2-6060","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-a939a886c7.jpg?v=1791767016"},{"product_id":"human-primary-diabetic-dermal-microvascular-endothelial-cells","title":"Human Primary Diabetic Dermal Microvascular Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eHuman Diabetic Primary Dermal Microvascular Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. HD2-6064\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Dermal Microvascular Endothelial Cells from Cell Biologics are isolated from human skin tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Human Diabetic Primary Dermal Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for Endothelial Cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Dermal Microvascular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003c\/p\u003e\u003cp\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527701418268,"sku":"HD2-6064","price":850.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687848104220,"sku":"HD2-6064","price":912.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687848136988,"sku":"HD2-6064","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-a939a886c7.jpg?v=1791767016"},{"product_id":"human-primary-diabetic-lymphatic-endothelial-cells","title":"Human Primary Diabetic Lymphatic Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eHuman Diabetic Primary Lymphatic Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. HD2-6092\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Lymphatic Endothelial Cells from Cell Biologics are isolated from human lymph node tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Human Diabetic Primary Lymphatic Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for Endothelial Cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Lymphatic Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003c\/p\u003e\u003cp\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527702270236,"sku":"HD2-6092","price":887.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687849546012,"sku":"HD2-6092","price":950.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687849578780,"sku":"HD2-6092","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-a939a886c7.jpg?v=1791767016"},{"product_id":"human-primary-diabetic-coronary-artery-endothelial-cells","title":"Human Primary Diabetic Coronary Artery Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eHuman Diabetic Primary Coronary Artery Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. HD2-6093\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Coronary Artery Endothelial Cells from Cell Biologics are isolated from human coronary artery tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Human Diabetic Primary Coronary Artery Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for Endothelial Cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eHuman Diabetic Primary Coronary Artery Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003c\/p\u003e\u003cp\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527702368540,"sku":"HD2-6093","price":887.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687849611548,"sku":"HD2-6093","price":950.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687849644316,"sku":"HD2-6093","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-a939a886c7.jpg?v=1791767016"},{"product_id":"human-primary-diabetic-gallbladder-endothelial-cells","title":"Human Primary Diabetic Gallbladder Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eHuman Primary Diabetic Gallbladder Endothelial Cells (HDGAEC)\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eHD2-6279\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=h1168\"\u003eComplete Human Endothelial Cell Medium– 500 ML\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003e\u003ca href=\"\/search?q=h1168\"\u003eH1168\u003c\/a\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eProduct Description\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Diabetic Gallbladder Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from the gallbladder of human donor that has been diagnosed with diabetes type II disease. Human Primary Diabetic Gallbladder Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, Cells at passage 3 are detached from flasks and immediately cryopreserved in vails. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. \u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eProduct Testing\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Diabetic Gallbladder Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e display typical cobblestone with large dark nuclei appearance under light microscopy. Cells are tested for expression of endothelial cell marker using antibody, CD31 (Catalog No. 550389, BD; CD31\/PECAM-1 PE-conjugated Antibody, Catalog No. FAB3567P, R\u0026amp;D) or VE-Cadherin (FITC-VE-cadherin Catalog No. 560411, BD) by immunofluorescence staining or FACS. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and\/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased. Cells can be expanded for 3-5 passages under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eLaboratory Applications\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Diabetic Gallbladder Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman Primary Diabetic Gallbladder Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eDisclaimer\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003ca href=\"http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm\" target=\"_blank\" rel=\"noopener\"\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm\" target=\"_blank\" rel=\"noopener\"\u003e\u003cbr\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003cu\u003eWarranty and Liability\u003c\/u\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003cem\u003eCell Biologics’\u003c\/em\u003e human endothelial cells with \u003cem\u003eCell Biologics’\u003c\/em\u003e Media (catalog number H1168)and Coating Solution for appropriate endothelial cell culture and cell testing following \u003cem\u003eCell Biologics’\u003c\/em\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527702827292,"sku":"HD2-6279","price":887.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687850266908,"sku":"HD2-6279","price":950.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687850299676,"sku":"HD2-6279","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-48828a3791.jpg?v=1791767016"}],"url":"https:\/\/jkpgye-36.myshopify.com\/collections\/human-diabetic-endothelial-cells-type-ii.oembed","provider":"Cell Biologics","version":"1.0","type":"link"}