{"title":"Engineered Cells","description":"\u003cp\u003eImmortalized, fluorescent (GFP, RFP), luciferase, Cre- and Cas9-expressing cell lines derived from Cell Biologics primary cells, across eight species. Knockout, stable-overexpression and shRNA-knockdown lines are made to order.\u003c\/p\u003e","products":[{"product_id":"cas9-expressing-c57bl-6-mouse-primary-colonic-smooth-muscle-cells","title":"Cas9-Expressing C57BL\/6 Mouse Primary Colonic Smooth Muscle Cells","description":"\u003cp\u003e\u003cstrong\u003eCas9-Expressing Mouse Primary Colonic Smooth Muscle Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6001Cas9\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2268 Complete Smooth Muscle Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCas9-Expressing Mouse Primary Colonic Smooth Muscle Cells from Cell Biologics are isolated from the colonic tissues of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Cas9-Expressing Mouse Primary Colonic Smooth Muscle Cells are characterized by immunofluorescent staining with antibody of α-smooth muscle actin(A2547, Sigma). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eCell Biologics generates Cas9-expressing stable cells through transducing Cas9-lentiviral particles. Cas9-stable cells express wild-type Cas endonuclease.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCas9-Expressing Mouse Primary Colonic Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527533383964,"sku":"C57-6001Cas9","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-301d34a7bc.jpg?v=1791767087"},{"product_id":"cre-expressing-mouse-primary-colonic-smooth-muscle-cells","title":"Cre-Expressing Mouse Primary Colonic Smooth Muscle Cells","description":"\u003cp\u003e\u003cstrong\u003eCre-Expressing Mouse Primary Colonic Smooth Muscle Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6001Cre\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2268 Complete Smooth Muscle Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCre-Expressing Mouse Primary Colonic Smooth Muscle Cells from Cell Biologics are isolated from the colonic tissues of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Cre-Expressing Mouse Primary Colonic Smooth Muscle Cells are characterized by immunofluorescent staining with antibody of α-smooth muscle actin. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eCell Biologics generates the Cre-expressing stable cells by transducing Cre recombinase. LOXP constructs are transfected into the stable cells that can be performed various biological analyses.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCre-Expressing Mouse Primary Colonic Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527533416732,"sku":"C57-6001Cre","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-301d34a7bc.jpg?v=1791767087"},{"product_id":"gfp-expressing-c57bl-6-mouse-primary-colonic-smooth-muscle-cells","title":"GFP-Expressing C57BL\/6 Mouse Primary Colonic Smooth Muscle Cells","description":"\u003cp\u003eFor Research Use Only\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eGFP-Expressing Mouse Primary Colonic Smooth Muscle Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6001GFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2268 Complete Smooth Muscle Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eGFP-Expressing Mouse Primary Colonic Smooth Muscle Cells from Cell Biologics are isolated from the colon tissues of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. GFP-Expressing Mouse Primary Colonic Smooth Muscle Cells are characterized by immunofluorescent staining with antibody of α-smooth muscle actin (A2547, Sigma). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eThe green fluorescent protein (GFP) gives rise to very bright green fluorescence when exposed to blue light. Cell Biologics generates various GFP-expressing stable cells including endothelial cells, epithelial cells and so forth. GFP-expressing cells are developed through transducing GFP-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eGFP-Expressing Mouse Primary Colonic Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527533449500,"sku":"C57-6001GFP","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-301d34a7bc.jpg?v=1791767087"},{"product_id":"immortalized-mouse-primary-colonic-smooth-muscle-cells","title":"Immortalized Mouse Primary Colonic Smooth Muscle Cells","description":"\u003cp\u003e\u003cstrong\u003eImmortalized Mouse Primary Colonic Smooth Muscle Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6001IM\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2268 Complete Smooth Muscle Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized Mouse Primary Colonic Smooth Muscle Cells from Cell Biologics are isolated from the colonic tissue of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Immortalized Mouse Primary Colonic Smooth Muscle Cells are characterized by immunofluorescent staining with antibody of α-smooth muscle actin. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for more than 10 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eImmortalized cells from Cell Biologics are derived from primary cells with extended life span. The primary cells are immortalized by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized Mouse Primary Colonic Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527533482268,"sku":"C57-6001IM","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-301d34a7bc.jpg?v=1791767087"},{"product_id":"immortalized-gfp-expressing-mouse-primary-colonic-smooth-muscle-cells","title":"Immortalized GFP-Expressing Mouse Primary Colonic Smooth Muscle Cells","description":"Immortalized GFP-Expressing Mouse Primary Colonic Smooth Muscle Cells\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527533515036,"sku":"C57-6001IM.GFP","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-301d34a7bc.jpg?v=1791767087"},{"product_id":"immortalized-rfp-expressing-mouse-primary-colonic-smooth-muscle-cells","title":"Immortalized RFP-Expressing Mouse Primary Colonic Smooth Muscle Cells","description":"\u003cp\u003eImmortalized RFP-Expressing Mouse Primary Colonic Smooth Muscle Cells - Mouse primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527533547804,"sku":"C57-6001IM.RFP","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-301d34a7bc.jpg?v=1791767087"},{"product_id":"luciferase-expressing-c57bl-6-mouse-primary-colonic-smooth-muscle-cells","title":"Luciferase-Expressing C57BL\/6 Mouse Primary Colonic Smooth Muscle Cells","description":"\u003cp\u003e\u003cstrong\u003eLuciferase-Expressing Mouse Primary Colonic Smooth Muscle Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6001Luc\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2268 Complete Smooth Muscle Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLuciferase-Expressing Mouse Primary Colonic Smooth Muscle Cells from Cell Biologics are isolated from the colonic tissues of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Luciferase-Expressing Mouse Primary Colonic Smooth Muscle Cells are characterized by immunofluorescent staining with antibody of α-smooth muscle actin (A2547, Sigma). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eLuciferase, distinct from GFP\/RFP photoprotein, is widely used in bioluminescence. Cell Biologics generates various Firefly luciferase-expressing stable cells , including endothelial cells, epithelial cells and so forth. Firefly luciferase-expressing cells are developed through transducing Firefly luciferase-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLuciferase-Expressing Mouse Primary Colonic Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527533613340,"sku":"C57-6001Luc","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-301d34a7bc.jpg?v=1791767087"},{"product_id":"rfp-expressing-c57bl-6-mouse-primary-colonic-smooth-muscle-cells","title":"RFP-Expressing C57BL\/6 Mouse Primary Colonic Smooth Muscle Cells","description":"\u003cp\u003e\u003cstrong\u003eRFP-Expressing Mouse Primary Colonic Smooth Muscle Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6001RFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2268 Complete Smooth Muscle Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRFP-Expressing Mouse Primary Colonic Smooth Muscle Cells from Cell Biologics are isolated from the colonic tissues of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. RFP-Expressing Mouse Primary Colonic Smooth Muscle Cells are characterized by immunofluorescent staining with antibody of α-smooth muscle actin (A2547, Sigma). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eRed Fluorescent Protein (RFP), a mutant form of green fluorescent protein, gives rise to very bright red fluorescence when exposed to yellow-green light. Cell Biologics generates various RFP-expressing stable cells, including endothelial cells, epithelial cells and so forth. RFP-expressing cells are developed through transducing RFP-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRFP-Expressing Mouse Primary Colonic Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527533646108,"sku":"C57-6001RFP","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-301d34a7bc.jpg?v=1791767087"},{"product_id":"cas9-expressing-c57bl-6-mouse-primary-carotid-artery-endothelial-cells","title":"Cas9-Expressing C57BL\/6 Mouse Primary Carotid Artery Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eCas9-Expressing Mouse Primary Artery Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6008Cas9\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCas9-Expressing Mouse Primary Artery Endothelial Cells from Cell Biologics are isolated from the artery tissues of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Cas9-Expressing Mouse Primary Artery Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended. \u003c\/p\u003e\u003cp\u003eCell Biologics generates Cas9-expressing stable cells through transducing Cas9-lentiviral particles. Cas9-stable cells express wild-type Cas endonuclease.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCas9-Expressing Mouse Primary Artery Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527533711644,"sku":"C57-6008Cas9","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-95e26eff29.jpg?v=1791767087"},{"product_id":"cre-expressing-mouse-primary-carotid-artery-endothelial-cells","title":"Cre-Expressing Mouse Primary Carotid Artery Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eCre-Expressing Mouse Primary Artery Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6008Cre\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCre-Expressing Mouse Primary Artery Endothelial Cells from Cell Biologics are isolated from the artery tissues of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Cre-Expressing Mouse Primary Artery Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended. \u003c\/p\u003e\u003cp\u003eCell Biologics generates the Cre-expressing stable cells by transducing Cre recombinase. LOXP constructs are transfected into the stable cells that can be performed various biological analyses.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCre-Expressing Mouse Primary Artery Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527533744412,"sku":"C57-6008Cre","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-95e26eff29.jpg?v=1791767087"},{"product_id":"gfp-expressing-c57bl-6-mouse-primary-carotid-artery-endothelial-cells","title":"GFP-Expressing C57BL\/6 Mouse Primary Carotid Artery Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eGFP-Expressing Mouse Primary Artery Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6008GFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eGFP-Expressing Mouse Primary Artery Endothelial Cells from Cell Biologics are isolated from the artery tissue of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. GFP-Expressing Mouse Primary Artery Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended. \u003c\/p\u003e\u003cp\u003eThe green fluorescent protein (GFP) gives rise to very bright green fluorescence when exposed to blue light. Cell Biologics generates various GFP-expressing stable cells, including Macrophages, epithelial cells and so forth. GFP-expressing cells are developed through transducing GFP-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eGFP-Expressing Mouse Primary Artery Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527533777180,"sku":"C57-6008GFP","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-95e26eff29.jpg?v=1791767087"},{"product_id":"immortalized-mouse-primary-carotid-artery-endothelial-cells","title":"Immortalized Mouse Primary Carotid Artery Endothelial Cells","description":"\u003cp\u003eFor Research Use Only\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eImmortalized Mouse Primary Artery Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6008IM\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized Mouse Primary Artery Endothelial Cells from Cell Biologics are isolated from the artery tissues of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Immortalized Mouse Primary Artery Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for more than 10 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. \u003c\/p\u003e\u003cp\u003eImmortalized cell lines from Cell Biologics were derived from primary cells with extended life span. The primary cells were immortalized by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized Mouse Primary Artery Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527533809948,"sku":"C57-6008IM","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-95e26eff29.jpg?v=1791767087"},{"product_id":"immortalized-gfp-expressing-mouse-primary-carotid-artery-endothelial-cells","title":"Immortalized GFP-Expressing Mouse Primary Carotid Artery Endothelial Cells","description":"Immortalized GFP-Expressing Mouse Primary Carotid Artery Endothelial Cells\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527533842716,"sku":"C57-6008IM.GFP","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-95e26eff29.jpg?v=1791767087"},{"product_id":"immortalized-rfp-expressing-mouse-primary-carotid-artery-endothelial-cells","title":"Immortalized RFP-Expressing Mouse Primary Carotid Artery Endothelial Cells","description":"\u003cp\u003eImmortalized RFP-Expressing Mouse Primary Carotid Artery Endothelial Cells - Mouse primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527533875484,"sku":"C57-6008IM.RFP","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-95e26eff29.jpg?v=1791767087"},{"product_id":"luciferase-expressing-c57bl-6-mouse-primary-carotid-artery-endothelial-cells","title":"Luciferase-Expressing C57BL\/6 Mouse Primary Carotid Artery Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eLuciferase-Expressing Mouse Primary Artery Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6008Luc\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLuciferase-Expressing Mouse Primary Artery Endothelial Cells from Cell Biologics are isolated from the artery tissues of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Luciferase-Expressing Mouse Primary Artery Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended. \u003c\/p\u003e\u003cp\u003eLuciferase, distinct from GFP\/RFP photoprotein, is widely used in bioluminescence. Cell Biologics generates various Firefly luciferase-expressing stable cells, including endothelial cells, epithelial cells and so forth. Firefly luciferase-expressing cells are developed through transducing Firefly luciferase-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLuciferase-Expressing Mouse Primary Artery Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527533908252,"sku":"C57-6008Luc","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-95e26eff29.jpg?v=1791767087"},{"product_id":"rfp-expressing-c57bl-6-mouse-primary-carotid-artery-endothelial-cells","title":"RFP-Expressing C57BL\/6 Mouse Primary Carotid Artery Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eRFP-Expressing Mouse Primary Artery Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6008RFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRFP-Expressing Mouse Primary Artery Endothelial Cells from Cell Biologics are isolated from the artery tissues of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. RFP-Expressing Mouse Primary Artery Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. \u003c\/p\u003e\u003cp\u003eRed Fluorescent Protein (RFP), a mutant form of green fluorescent protein, gives rise to very bright red fluorescence when exposed to yellow-green light. Cell Biologics generates various RFP-expressing stable cells, including endothelial cells, epithelial cells and so forth. RFP-expressing cells are developed through transducing RFP-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRFP-Expressing Mouse Primary Artery Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527533941020,"sku":"C57-6008RFP","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-95e26eff29.jpg?v=1791767087"},{"product_id":"cas9-expressing-c57bl-6-mouse-primary-vein-endothelial-cells","title":"Cas9-Expressing C57BL\/6 Mouse Primary Vein Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eCas9-Expressing Mouse Primary Vein Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6009Cas9\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCas9-Expressing Mouse Primary Vein Endothelial Cells from Cell Biologics are isolated from vein tissue of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Cas9-Expressing Mouse Primary Vein Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003eCell Biologics generates Cas9-expressing stable cells through transducing Cas9-lentiviral particles. Cas9-stable cells express wild-type Cas endonuclease.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCas9-Expressing Mouse Primary Vein Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534039324,"sku":"C57-6009Cas9","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-d2834e11ef.jpg?v=1791767087"},{"product_id":"cre-expressing-mouse-primary-vein-endothelial-cells","title":"Cre-Expressing Mouse Primary Vein Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eCre-Expressing Mouse Primary Vein Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6009Cre\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCre-Expressing Mouse Primary Vein Endothelial Cells from Cell Biologics are isolated from vein tissue of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Cre-Expressing Mouse Primary Vein Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003eCell Biologics generates the Cre-expressing stable cells by transducing Cre recombinase. LOXP constructs are transfected into the stable cells that can be performed various biological analyses.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCre-Expressing Mouse Primary Vein Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534072092,"sku":"C57-6009Cre","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-d2834e11ef.jpg?v=1791767087"},{"product_id":"gfp-expressing-c57bl-6-mouse-primary-vein-endothelial-cells","title":"GFP-Expressing C57BL\/6 Mouse Primary Vein Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eGFP-Expressing Mouse Primary Vein Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6009GFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eGFP-Expressing Mouse Primary Vein Endothelial Cells from Cell Biologics are isolated from vein tissue of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. GFP-Expressing Mouse Primary Vein Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003eThe green fluorescent protein (GFP) gives rise to very bright green fluorescence when exposed to blue light. Cell Biologics generates various GFP-expressing stable cells, including Macrophages, epithelial cells and so forth. GFP-expressing cells are developed through transducing GFP-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eGFP-Expressing Mouse Primary Vein Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534104860,"sku":"C57-6009GFP","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-d2834e11ef.jpg?v=1791767087"},{"product_id":"immortalized-mouse-primary-vein-endothelial-cells","title":"Immortalized Mouse Primary Vein Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eImmortalized Mouse Primary Vein Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6009IM\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized Mouse Primary Vein Endothelial Cells from Cell Biologics are isolated vein tissue of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Immortalized Mouse Primary Vein Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for more than 10 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eImmortalized cell lines from Cell Biologics were derived from primary cells with extended life span. The primary cells were immortalized by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized Mouse Primary Vein Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534137628,"sku":"C57-6009IM","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-d2834e11ef.jpg?v=1791767087"},{"product_id":"immortalized-gfp-expressing-mouse-primary-vein-endothelial-cells","title":"Immortalized GFP-Expressing Mouse Primary Vein Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eImmortalized GFP-Expressing Mouse Primary Vein Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6009IM.GFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Mouse Primary Vein Endothelial Cells from Cell Biologics are isolated vein tissue of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Immortalized GFP-Expressing Mouse Primary Vein Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for more than 10 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eImmortalized cell lines from Cell Biologics were derived from primary cells expressing GFP. The primary cells were immortalized by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Mouse Primary Vein Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534170396,"sku":"C57-6009IM.GFP","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-d2834e11ef.jpg?v=1791767087"},{"product_id":"immortalized-rfp-expressing-mouse-primary-vein-endothelial-cells","title":"Immortalized RFP-Expressing Mouse Primary Vein Endothelial Cells","description":"\u003cp\u003eImmortalized RFP-Expressing Mouse Primary Vein Endothelial Cells - Mouse primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534203164,"sku":"C57-6009IM.RFP","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-d2834e11ef.jpg?v=1791767087"},{"product_id":"luciferase-expressing-c57bl-6-mouse-primary-vein-endothelial-cells","title":"Luciferase-Expressing C57BL\/6 Mouse Primary Vein Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eLuciferase-Expressing Mouse Primary Vein Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6009Luc\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLuciferase-Expressing Mouse Primary Vein Endothelial Cells from Cell Biologics are isolated from vein tissue of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 1x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Luciferase-Expressing Mouse Primary Vein Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003eLuciferase, distinct from GFP\/RFP photoprotein, is widely used in bioluminescence. Cell Biologics generates various Firefly luciferase-expressing stable cells, including endothelial cells, epithelial cells and so forth. Firefly luciferase-expressing cells are developed through transducing Firefly luciferase-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLuciferase-Expressing Mouse Primary Vein Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534235932,"sku":"C57-6009Luc","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-d2834e11ef.jpg?v=1791767087"},{"product_id":"rfp-expressing-c57bl-6-mouse-primary-vein-endothelial-cells","title":"RFP-Expressing C57BL\/6 Mouse Primary Vein Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eRFP-Expressing Mouse Primary Vein Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6009RFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRFP-Expressing Mouse Primary Vein Endothelial Cells from Cell Biologics are isolated from the vein tissue of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. RFP-Expressing Mouse Primary Vein Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eRed Fluorescent Protein (RFP), a mutant form of green fluorescent protein, gives rise to very bright red fluorescence when exposed to yellow-green light. Cell Biologics generates various RFP-expressing stable cells, including endothelial cells, epithelial cells and so forth. RFP-expressing cells are developed through transducing RFP-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRFP-Expressing Mouse Primary Vein Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534268700,"sku":"C57-6009RFP","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-d2834e11ef.jpg?v=1791767087"},{"product_id":"cas9-expressing-c57bl-6-mouse-primary-lung-microvascular-endothelial-cells","title":"Cas9-Expressing C57BL\/6 Mouse Primary Lung Microvascular Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eCas9-Expressing Mouse Primary Lung Microvascular Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6011Cas9\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCas9-Expressing Mouse Primary Lung Microvascular Endothelial Cells from Cell Biologics are isolated from lung tissue of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Cas9-Expressing Mouse Primary Lung Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003eCell Biologics generates Cas9-expressing stable cells through transducing Cas9-lentiviral particles. Cas9-stable cells express wild-type Cas endonuclease.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCas9-Expressing Mouse Primary Lung Microvascular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534334236,"sku":"C57-6011Cas9","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-3a20bdfe49.jpg?v=1791767087"},{"product_id":"cre-expressing-mouse-primary-lung-microvascular-endothelial-cells","title":"Cre-Expressing Mouse Primary Lung Microvascular Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eCre-Expressing Mouse Primary Lung Microvascular Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6011Cre\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCre-Expressing Mouse Primary Lung Microvascular Endothelial Cells from Cell Biologics are isolated from lung tissue of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Cre-Expressing Mouse Primary Lung Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003eCell Biologics generates the Cre-expressing stable cells by transducing Cre recombinase. LOXP constructs are transfected into the stable cells that can be performed various biological analyses.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCre-Expressing Mouse Primary Lung Microvascular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534367004,"sku":"C57-6011Cre","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-3a20bdfe49.jpg?v=1791767087"},{"product_id":"gfp-expressing-c57bl-6-mouse-primary-lung-microvascular-endothelial-cells","title":"GFP-Expressing C57BL\/6 Mouse Primary Lung Microvascular Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eGFP-Expressing Mouse Primary Lung Microvascular Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6011GFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eGFP-Expressing Mouse Primary Lung Microvascular Endothelial Cells from Cell Biologics are isolated from lung tissue of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. GFP-Expressing Mouse Primary Lung Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003eThe green fluorescent protein (GFP) gives rise to very bright green fluorescence when exposed to blue light. Cell Biologics generates various GFP-expressing stable cells, including Macrophages, epithelial cells and so forth. GFP-expressing cells are developed through transducing GFP-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eGFP-Expressing Mouse Primary Lung Microvascular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534399772,"sku":"C57-6011GFP","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-3a20bdfe49.jpg?v=1791767087"},{"product_id":"immortalized-mouse-primary-lung-microvascular-endothelial-cells","title":"Immortalized Mouse Primary Lung Microvascular Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eImmortalized Mouse Primary Lung Microvascular Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6011IM\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized Mouse Primary Lung Microvascular Endothelial Cells from Cell Biologics are isolated from lung tissue of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Immortalized Mouse Primary Lung Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for more than 10 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eImmortalized cell lines from Cell Biologics were derived from primary cells with extended life span. The primary cells were immortalized by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized Mouse Primary Lung Microvascular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534432540,"sku":"C57-6011IM","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-3a20bdfe49.jpg?v=1791767087"},{"product_id":"immortalized-gfp-expressing-mouse-primary-lung-microvascular-endothelial-cells","title":"Immortalized GFP-Expressing Mouse Primary Lung Microvascular Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eImmortalized GFP-Expressing Mouse Primary Lung Microvascular Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6011IM.GFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Mouse Primary Lung Microvascular Endothelial Cells from Cell Biologics are isolated from Lung tissue of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Immortalized GFP-Expressing Mouse Primary Lung Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for more than 10 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eImmortalized cell lines from Cell Biologics were derived from primary cells expressing GFP. The primary cells were immortalized by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Mouse Primary Lung Microvascular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534465308,"sku":"C57-6011IM.GFP","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-3a20bdfe49.jpg?v=1791767087"},{"product_id":"immortalized-rfp-expressing-mouse-primary-lung-microvascular-endothelial-cells","title":"Immortalized RFP-Expressing Mouse Primary Lung Microvascular Endothelial Cells","description":"\u003cp\u003eImmortalized RFP-Expressing Mouse Primary Lung Microvascular Endothelial Cells - Mouse primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534498076,"sku":"C57-6011IM.RFP","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-3a20bdfe49.jpg?v=1791767087"},{"product_id":"luciferase-expressing-c57bl-6-mouse-primary-lung-microvascular-endothelial-cells","title":"Luciferase-Expressing C57BL\/6 Mouse Primary Lung Microvascular Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eLuciferase-Expressing Mouse Primary Lung Microvascular Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6011Luc\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLuciferase-Expressing Mouse Primary Lung Microvascular Endothelial Cells from Cell Biologics are isolated from lung tissues of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 1x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Luciferase-Expressing Mouse Primary Lung Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003eLuciferase, distinct from GFP\/RFP photoprotein, is widely used in bioluminescence. Cell Biologics generates various Firefly luciferase-expressing stable cells, including endothelial cells, epithelial cells and so forth. Firefly luciferase-expressing cells are developed through transducing Firefly luciferase-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLuciferase-Expressing Mouse Primary Lung Microvascular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534530844,"sku":"C57-6011Luc","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-3a20bdfe49.jpg?v=1791767087"},{"product_id":"rfp-expressing-c57bl-6-mouse-primary-lung-microvascular-endothelial-cells","title":"RFP-Expressing C57BL\/6 Mouse Primary Lung Microvascular Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eRFP-Expressing Mouse Primary Lung Microvascular Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6011RFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRFP-Expressing Mouse Primary Lung Microvascular Endothelial Cells from Cell Biologics are isolated from the lung tissues of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. RFP-Expressing Mouse Primary Lung Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eRed Fluorescent Protein (RFP), a mutant form of green fluorescent protein, gives rise to very bright red fluorescence when exposed to yellow-green light. Cell Biologics generates various RFP-expressing stable cells, including endothelial cells, epithelial cells and so forth. RFP-expressing cells are developed through transducing RFP-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRFP-Expressing Mouse Primary Lung Microvascular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534563612,"sku":"C57-6011RFP","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-3a20bdfe49.jpg?v=1791767087"},{"product_id":"cas9-expressing-c57bl-6-mouse-primary-lung-fibroblasts","title":"Cas9-Expressing C57BL\/6 Mouse Primary Lung Fibroblasts","description":"\u003cp\u003e\u003cstrong\u003eCas9-Expressing Mouse Primary Lung Fibroblasts\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6013Cas9\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2267 Complete Fibroblast Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCas9-Expressing Mouse Primary Lung Fibroblasts from Cell Biologics are isolated from the lung tissues of pathogen-free laboratory mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Cas9-Expressing Mouse Primary Lung Fibroblasts are characterized by their spindle morphology and immunofluorescence staining with anti-FSP1\/S100A4 (Millipore USA) antibody. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eCell Biologics generates Cas9-expressing stable cells through transducing Cas9-lentiviral particles. Cas9-stable cells express wild-type Cas endonuclease.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCas9-Expressing Mouse Primary Lung Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534661916,"sku":"C57-6013Cas9","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-16c0aba89f.jpg?v=1791767054"},{"product_id":"cre-expressing-mouse-primary-lung-fibroblasts","title":"Cre-Expressing Mouse Primary Lung Fibroblasts","description":"\u003cp\u003e\u003cstrong\u003eCRE-Expressing Mouse Primary Lung Fibroblasts\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6013Cre\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2267 Complete Fibroblast Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCRE-Expressing Mouse Primary Lung Fibroblasts from Cell Biologics are isolated from the lung tissues of pathogen-free laboratory mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. CRE-Expressing Mouse Primary Lung Fibroblasts are characterized by their spindle morphology and immunofluorescence staining with anti-FSP1\/S100A4 (Millipore USA) antibody. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eCell Biologics generates the Cre-expressing stable cells by transducing Cre recombinase. LOXP constructs are transfected into the stable cells that can be performed various biological analyses.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCRE-Expressing Mouse Primary Lung Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534694684,"sku":"C57-6013Cre","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-16c0aba89f.jpg?v=1791767054"},{"product_id":"gfp-expressing-c57bl-6-mouse-primary-lung-fibroblasts","title":"GFP-Expressing C57BL\/6 Mouse Primary Lung Fibroblasts","description":"\u003cp\u003e\u003cstrong\u003eGFP-Expressing Mouse Primary Lung Fibroblasts\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6013GFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2267 Complete Fibroblast Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eGFP-Expressing Mouse Primary Lung Fibroblasts from Cell Biologics are isolated from the lung tissues of pathogen-free laboratory mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. GFP-Expressing Mouse Primary Lung Fibroblasts are characterized by their spindle morphology and immunofluorescence staining with anti-FSP1\/S100A4 (Millipore USA) antibody. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eThe green fluorescent protein (GFP) gives rise to very bright green fluorescence when exposed to blue light. Cell Biologics generates various GFP-expressing stable cells, including endothelial cells, epithelial cells and so forth. GFP-expressing cells are developed through transducing GFP-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eGFP-Expressing Mouse Primary Lung Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534727452,"sku":"C57-6013GFP","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-16c0aba89f.jpg?v=1791767054"},{"product_id":"immortalized-mouse-primary-lung-fibroblasts","title":"Immortalized Mouse Primary Lung Fibroblasts","description":"\u003cp\u003e\u003cstrong\u003eImmortalized Mouse Primary Lung Fibroblasts\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6013IM\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2267 Complete Fibroblast Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized Mouse Primary Lung Fibroblasts from Cell Biologics are isolated from the lung tissues of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Immortalized Mouse Primary Lung Fibroblasts are characterized by their spindle morphology and immunofluorescence staining with anti-FSP1\/S100A4 (Millipore USA) antibody. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for more than 10 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eImmortalized cells from Cell Biologics are derived from primary cells with extended life span. The primary cells are immortalized by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized Mouse Primary Lung Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534760220,"sku":"C57-6013IM","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-16c0aba89f.jpg?v=1791767054"},{"product_id":"immortalized-gfp-expressing-mouse-primary-lung-fibroblasts","title":"Immortalized GFP-Expressing Mouse Primary Lung Fibroblasts","description":"Immortalized GFP-Expressing Mouse Primary Lung Fibroblasts\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534792988,"sku":"C57-6013IM.GFP","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-16c0aba89f.jpg?v=1791767054"},{"product_id":"immortalized-rfp-expressing-mouse-primary-lung-fibroblasts","title":"Immortalized RFP-Expressing Mouse Primary Lung Fibroblasts","description":"\u003cp\u003eImmortalized RFP-Expressing Mouse Primary Lung Fibroblasts - Mouse primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534825756,"sku":"C57-6013IM.RFP","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-16c0aba89f.jpg?v=1791767054"},{"product_id":"luciferase-expressing-c57bl-6-mouse-primary-lung-fibroblasts","title":"Luciferase-Expressing C57BL\/6 Mouse Primary Lung Fibroblasts","description":"\u003cp\u003e\u003cstrong\u003eLuciferase-Expressing Mouse Primary Lung Fibroblasts\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6013Luc\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2267 Complete Fibroblast Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLuciferase-Expressing Mouse Primary Lung Fibroblasts from Cell Biologics are isolated from the lung tissues of pathogen-free laboratory mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Luciferase-Expressing Mouse Primary Lung Fibroblasts are characterized by their spindle morphology and immunofluorescence staining with anti-FSP1\/S100A4 (Millipore USA) antibody. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eLuciferase, distinct from GFP\/RFP photoprotein, is widely used in bioluminescence. Cell Biologics generates various Firefly luciferase-expressing stable cells, including endothelial cells, epithelial cells and so forth. Firefly luciferase-expressing cells are developed through transducing Firefly luciferase-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLuciferase-Expressing Mouse Primary Lung Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534858524,"sku":"C57-6013Luc","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-16c0aba89f.jpg?v=1791767054"},{"product_id":"rfp-expressing-c57bl-6-mouse-primary-lung-fibroblasts","title":"RFP-Expressing C57BL\/6 Mouse Primary Lung Fibroblasts","description":"RFP Expressing C57BL\/6 Mouse Primary Lung Fibroblasts\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534891292,"sku":"C57-6013RFP","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-16c0aba89f.jpg?v=1791767054"},{"product_id":"cas9-expressing-c57bl-6-mouse-primary-kidney-endothelial-cells","title":"Cas9-Expressing C57BL\/6 Mouse Primary Kidney Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eCas9-Expressing Mouse Primary Kidney Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6014Cas9\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCas9-Expressing Mouse Primary Kidney Endothelial Cells from Cell Biologics are isolated from kidney tissue of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Cas9-Expressing Mouse Primary Kidney Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended. \u003c\/p\u003e\u003cp\u003eCell Biologics generates Cas9-expressing stable cells through transducing Cas9-lentiviral particles. Cas9-stable cells express wild-type Cas endonuclease.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCas9-Expressing Mouse Primary Kidney Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527534989596,"sku":"C57-6014Cas9","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff5cea3fab.jpg?v=1791767017"},{"product_id":"cre-expressing-mouse-primary-kidney-endothelial-cells","title":"Cre-Expressing Mouse Primary Kidney Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eCre-Expressing Mouse Primary Kidney Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6014Cre\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCre-Expressing Mouse Primary Kidney Endothelial Cells from Cell Biologics are isolated from kidney of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Cre-Expressing Mouse Primary Kidney Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003eCell Biologics generates the Cre-expressing stable cells by transducing Cre recombinase. LOXP constructs are transfected into the stable cells that can be performed various biological analyses.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCre-Expressing Mouse Primary Kidney Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527535022364,"sku":"C57-6014Cre","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff5cea3fab.jpg?v=1791767017"},{"product_id":"cas9-expressing-c57bl-6-mouse-primary-kidney-glomerular-endothelial-cells","title":"Cas9-Expressing C57BL\/6 Mouse Primary Kidney Glomerular Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eCas9-Expressing Mouse Primary Kidney Glomerular Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6014G.Cas9\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCas9-Expressing Mouse Primary Kidney Glomerular Endothelial Cells from Cell Biologics are isolated from Kidney Glomerular tissue of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Cas9-Expressing Mouse Primary Kidney Glomerular Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003eCell Biologics generates Cas9-expressing stable cells through transducing Cas9-lentiviral particles. Cas9-stable cells express wild-type Cas endonuclease.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCas9-Expressing Mouse Primary Kidney Glomerular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527535087900,"sku":"C57-6014G.Cas9","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff5cea3fab.jpg?v=1791767017"},{"product_id":"cre-expressing-mouse-primary-kidney-glomerular-endothelial-cells","title":"Cre-Expressing Mouse Primary Kidney Glomerular Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eCre-Expressing Mouse Primary Kidney Glomerular Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6014G.Cre\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCre-Expressing Mouse Primary Kidney Glomerular Endothelial Cells from Cell Biologics are isolated from kidney glomerular tissue of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Cre-Expressing Mouse Primary Kidney Glomerular Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003eCell Biologics generates the Cre-expressing stable cells by transducing Cre recombinase. LOXP constructs are transfected into the stable cells that can be performed various biological analyses.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCre-Expressing Mouse Primary Kidney Glomerular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527535120668,"sku":"C57-6014G.Cre","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-7d90943525.jpg?v=1791766977"},{"product_id":"gfp-expressing-c57bl-6-mouse-primary-kidney-glomerular-endothelial-cells","title":"GFP-Expressing C57BL\/6 Mouse Primary Kidney Glomerular Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eGFP-Expressing Mouse Primary Kidney Glomerular Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6014G.GFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eGFP-Expressing Mouse Primary Kidney Glomerular Endothelial Cells from Cell Biologics are isolated from Kidney Glomerular tissue of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. GFP-Expressing Mouse Primary Kidney Glomerular Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003eThe green fluorescent protein (GFP) gives rise to very bright green fluorescence when exposed to blue light. Cell Biologics generates various GFP-expressing stable cells, including Macrophages, epithelial cells and so forth. GFP-expressing cells are developed through transducing GFP-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eGFP-Expressing Mouse Primary Kidney Glomerular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527535153436,"sku":"C57-6014G.GFP","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-7d90943525.jpg?v=1791766977"},{"product_id":"immortalized-mouse-primary-kidney-glomerular-endothelial-cells","title":"Immortalized Mouse Primary Kidney Glomerular Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eImmortalized GFP-Expressing Mouse Primary Kidney Glomerular Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6014G.IM.GFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Mouse Primary Kidney Glomerular Endothelial Cells from Cell Biologics are isolated from Kidney Glomerular tissue of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Immortalized GFP-Expressing Mouse Primary Kidney Glomerular Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for more than 10 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eImmortalized cell lines from Cell Biologics were derived from primary cells expressing GFP. The primary cells were immortalized by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Mouse Primary Kidney Glomerular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527535186204,"sku":"C57-6014G.IM","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff5cea3fab.jpg?v=1791767017"},{"product_id":"immortalized-gfp-expressing-mouse-primary-kidney-glomerular-endothelial-cells","title":"Immortalized GFP-Expressing Mouse Primary Kidney Glomerular Endothelial Cells","description":"Immortalized GFP-Expressing Mouse Primary Kidney Glomerular Endothelial Cells\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527535218972,"sku":"C57-6014G.IM.GFP","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff5cea3fab.jpg?v=1791767017"},{"product_id":"immortalized-rfp-expressing-mouse-primary-kidney-glomerular-endothelial-cells","title":"Immortalized RFP-Expressing Mouse Primary Kidney Glomerular Endothelial Cells","description":"\u003cp\u003eImmortalized RFP-Expressing Mouse Primary Kidney Glomerular Endothelial Cells - Mouse primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527535251740,"sku":"C57-6014G.IM.RFP","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff5cea3fab.jpg?v=1791767017"},{"product_id":"luciferase-expressing-c57bl-6-mouse-primary-kidney-glomerular-endothelial-cells","title":"Luciferase-Expressing C57BL\/6 Mouse Primary Kidney Glomerular Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eLuciferase-Expressing Mouse Primary Kidney Glomerular Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6014G.Luc\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLuciferase-Expressing Mouse Primary Kidney Glomerular Endothelial Cells from Cell Biologics are isolated from kidney tissues of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 1x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Luciferase-Expressing Mouse Primary Kidney Glomerular Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003eLuciferase, distinct from GFP\/RFP photoprotein, is widely used in bioluminescence. Cell Biologics generates various Firefly luciferase-expressing stable cells, including endothelial cells, epithelial cells and so forth. Firefly luciferase-expressing cells are developed through transducing Firefly luciferase-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLuciferase-Expressing Mouse Primary Kidney Glomerular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527535284508,"sku":"C57-6014G.Luc","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-7d90943525.jpg?v=1791766977"},{"product_id":"rfp-expressing-c57bl-6-mouse-primary-kidney-glomerular-endothelial-cells","title":"RFP-Expressing C57BL\/6 Mouse Primary Kidney Glomerular Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eRFP-Expressing Mouse Primary Kidney Glomerular Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6014G.RFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRFP-Expressing Mouse Primary Kidney Glomerular Endothelial Cells from Cell Biologics are isolated from the kidney tissues of pathogen-free C57BL\/6 laboratory mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. RFP-Expressing Mouse Primary Kidney Glomerular Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eRed Fluorescent Protein (RFP), a mutant form of green fluorescent protein, gives rise to very bright red fluorescence when exposed to yellow-green light. Cell Biologics generates various RFP-expressing stable cells, including endothelial cells, epithelial cells and so forth. RFP-expressing cells are developed through transducing RFP-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRFP-Expressing Mouse Primary Kidney Glomerular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527535317276,"sku":"C57-6014G.RFP","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-7d90943525.jpg?v=1791766977"}],"url":"https:\/\/jkpgye-36.myshopify.com\/collections\/engineered-cells.oembed","provider":"Cell Biologics","version":"1.0","type":"link"}