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Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Canine Primary Colonic Smooth Muscle Cells are characterized by immunofluorescence staining with antibody of α-smooth muscle actin and negative for bacteria, yeast, fungi, and mycoplasma. These cells can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCanine Primary Colonic Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527580209436,"sku":"D-6001","price":610.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687324930332,"sku":"D-6001","price":660.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687324963100,"sku":"D-6001","price":980.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-781fc740f5.jpg?v=1791767016"},{"product_id":"cas9-expressing-canine-primary-colonic-smooth-muscle-cells","title":"Cas9-Expressing Canine Primary Colonic Smooth Muscle Cells","description":"\u003cp\u003eFor Research Use Only\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCas9-Expressing Canine Primary Colonic Smooth Muscle Cells \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. D-6001Cas9\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2268 Complete Smooth Muscle Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCas9-Expressing Canine Primary Colonic Smooth Muscle Cells from Cell Biologics are isolated from the colonic tissue of beagle dog and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Cas9-Expressing Canine Primary Colonic Smooth Muscle Cells are characterized by immunofluorescence staining with antibody of α-smooth muscle actin and negative for bacteria, yeast, fungi, and mycoplasma. These cells can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eCell Biologics generates Cas9-expressing stable cells through transducing Cas9-lentiviral particles. Cas9-stable cells express wild-type Cas endonuclease.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCas9-Expressing Canine Primary Colonic Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527580242204,"sku":"D-6001Cas9","price":1100.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-781fc740f5.jpg?v=1791767016"},{"product_id":"cre-expressing-canine-primary-colonic-smooth-muscle-cells","title":"Cre-Expressing Canine Primary Colonic Smooth Muscle Cells","description":"\u003cp\u003eFor Research Use Only\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCre-Expressing Canine Primary Colonic Smooth Muscle Cells \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. D-6001Cre\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2268 Complete Smooth Muscle Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCre-Expressing Canine Primary Colonic Smooth Muscle Cells from Cell Biologics are isolated from the colonic tissue of beagle dog and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Cre-Expressing Canine Primary Colonic Smooth Muscle Cells are characterized by immunofluorescence staining with antibody of α-smooth muscle actin and negative for bacteria, yeast, fungi, and mycoplasma. These cells can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eCell Biologics generates the Cre-expressing stable cells by transducing Cre recombinase. LOXP constructs are transfected into the stable cells that can be performed various biological analyses.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCre-Expressing Canine Primary Colonic Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527580274972,"sku":"D-6001Cre","price":1100.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-781fc740f5.jpg?v=1791767016"},{"product_id":"gfp-expressing-canine-primary-colonic-smooth-muscle-cells","title":"GFP-Expressing Canine Primary Colonic Smooth Muscle Cells","description":"\u003cp\u003eFor Research Use Only\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eGFP-Expressing Canine Primary Colonic Smooth Muscle Cells \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. D-6001GFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2268 Complete Smooth Muscle Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eGFP-Expressing Canine Primary Colonic Smooth Muscle Cells from Cell Biologics are isolated from the colonic tissue of beagle dog and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. GFP-Expressing Canine Primary Colonic Smooth Muscle Cells are characterized by immunofluorescence staining with antibody of α-smooth muscle actin and negative for bacteria, yeast, fungi, and mycoplasma. These cells can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eThe green fluorescent protein (GFP) gives rise to very bright green fluorescence when exposed to blue light. Cell Biologics generates various GFP-expressing stable cells. GFP-expressing cells are developed through transducing GFP-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eGFP-Expressing Canine Primary Colonic Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527580307740,"sku":"D-6001GFP","price":1100.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-781fc740f5.jpg?v=1791767016"},{"product_id":"immortalized-canine-primary-colonic-smooth-muscle-cells","title":"Immortalized Canine Primary Colonic Smooth Muscle Cells","description":"\u003cp\u003eFor Research Use Only\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eImmortalized Canine Primary Colonic Smooth Muscle Cells \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. D-6001IM\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2268 Complete Smooth Muscle Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized Canine Primary Colonic Smooth Muscle Cells from Cell Biologics are isolated from the colonic tissue of beagle dog and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Immortalized Canine Primary Colonic Smooth Muscle Cells are characterized by immunofluorescence staining with antibody of α-smooth muscle actin and negative for bacteria, yeast, fungi, and mycoplasma. These cells can be expanded for more than 10 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eImmortalized cells from Cell Biologics are derived from primary cells with extended life span. The primary cells are immortalized by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized Canine Primary Colonic Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527580340508,"sku":"D-6001IM","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-781fc740f5.jpg?v=1791767016"},{"product_id":"immortalized-gfp-expressing-canine-primary-colonic-smooth-muscle-cells","title":"Immortalized GFP-Expressing Canine Primary Colonic Smooth Muscle Cells","description":"\u003cp\u003eFor Research Use Only\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eImmortalized GFP-Expressing Canine Primary Colonic Smooth Muscle Cells \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. D-6001IM.GFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2268 Complete Smooth Muscle Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Canine Primary Colonic Smooth Muscle Cells from Cell Biologics are isolated from the colonic tissue of beagle dog and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Immortalized GFP-Expressing Canine Primary Colonic Smooth Muscle Cells are characterized by immunofluorescence staining with antibody of α-smooth muscle actin and negative for bacteria, yeast, fungi, and mycoplasma. These cells can be expanded for more than 10 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eImmortalized cells from Cell Biologics are derived from primary cells expressing GFP. These primary cells are immortalized by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Canine Primary Colonic Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527580373276,"sku":"D-6001IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-781fc740f5.jpg?v=1791767016"},{"product_id":"immortalized-rfp-expressing-canine-primary-colonic-smooth-muscle-cells","title":"Immortalized RFP-Expressing Canine Primary Colonic Smooth Muscle Cells","description":"\u003cp\u003eImmortalized RFP-Expressing Canine Primary Colonic Smooth Muscle Cells - Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527580406044,"sku":"D-6001IM.RFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-781fc740f5.jpg?v=1791767016"},{"product_id":"luciferase-expressing-canine-primary-colonic-smooth-muscle-cells","title":"Luciferase-Expressing Canine Primary Colonic Smooth Muscle Cells","description":"\u003cp\u003eFor Research Use Only\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eLuciferase-Expressing Canine Primary Colonic Smooth Muscle Cells \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. D-6001Luc\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2268 Complete Smooth Muscle Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLuciferase-Expressing Canine Primary Colonic Smooth Muscle Cells from Cell Biologics are isolated from the colonic tissue of beagle dog and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Luciferase-Expressing Canine Primary Colonic Smooth Muscle Cells are characterized by immunofluorescence staining with antibody of α-smooth muscle actin and negative for bacteria, yeast, fungi, and mycoplasma. These cells can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eLuciferase, distinct from GFP\/RFP photoprotein, is widely used in bioluminescence. Cell Biologics generates various Firefly luciferase-expressing stable cells, including endothelial cells, epithelial cells and so forth. Firefly luciferase-expressing cells are developed through transducing Firefly luciferase-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLuciferase-Expressing Canine Primary Colonic Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527580438812,"sku":"D-6001Luc","price":1100.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-781fc740f5.jpg?v=1791767016"},{"product_id":"rfp-expressing-canine-primary-colonic-smooth-muscle-cells","title":"RFP-Expressing Canine Primary Colonic Smooth Muscle Cells","description":"\u003cp\u003eFor Research Use Only\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRFP-Expressing Canine Primary Colonic Smooth Muscle Cells \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. D-6001RFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2268 Complete Smooth Muscle Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRFP-Expressing Canine Primary Colonic Smooth Muscle Cells from Cell Biologics are isolated from the colonic tissue of beagle dog and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. RFP-Expressing Canine Primary Colonic Smooth Muscle Cells are characterized by immunofluorescence staining with antibody of α-smooth muscle actin and negative for bacteria, yeast, fungi, and mycoplasma. These cells can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eRed Fluorescent Protein (RFP), a mutant form of green fluorescent protein, gives rise to very bright red fluorescence when exposed to yellow-green light. Cell Biologics generates various RFP-expressing stable cells, including endothelial cells, epithelial cells and so forth. RFP-expressing cells are developed through transducing RFP-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRFP-Expressing Canine Primary Colonic Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527580471580,"sku":"D-6001RFP","price":1100.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-781fc740f5.jpg?v=1791767016"},{"product_id":"canine-primary-carotid-artery-endothelial-cells","title":"Canine Primary Carotid Artery Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eCanine Primary Artery Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. D-6008\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCanine Primary Artery Endothelial Cells from Cell Biologics are isolated from the artery tissue of beagle dogs and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Canine Primary Artery Endothelial Cells are characterized by use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCanine Primary Artery Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527580504348,"sku":"D-6008","price":860.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-e00077625d.jpg?v=1791767054"},{"product_id":"cas9-expressing-canine-primary-carotid-artery-endothelial-cells","title":"Cas9-Expressing Canine Primary Carotid Artery Endothelial Cells","description":"\u003cp\u003eCas9-Expressing Canine Primary Carotid Artery Endothelial Cells - Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527580537116,"sku":"D-6008Cas9","price":1100.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-e00077625d.jpg?v=1791767054"},{"product_id":"cre-expressing-canine-primary-carotid-artery-endothelial-cells","title":"Cre-Expressing Canine Primary Carotid Artery Endothelial Cells","description":"\u003cp\u003eCre-Expressing Canine Primary Carotid Artery Endothelial Cells - Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527580569884,"sku":"D-6008Cre","price":1100.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-e00077625d.jpg?v=1791767054"},{"product_id":"gfp-expressing-canine-primary-carotid-artery-endothelial-cells","title":"GFP-Expressing Canine Primary Carotid Artery Endothelial Cells","description":"\u003cp\u003eGFP-Expressing Canine Primary Carotid Artery Endothelial Cells - Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527580602652,"sku":"D-6008GFP","price":1100.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-e00077625d.jpg?v=1791767054"},{"product_id":"immortalized-canine-primary-carotid-artery-endothelial-cells","title":"Immortalized Canine Primary Carotid Artery Endothelial Cells","description":"\u003cp\u003eImmortalized Canine Primary Carotid Artery Endothelial Cells - Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527580635420,"sku":"D-6008IM","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-e00077625d.jpg?v=1791767054"}],"url":"https:\/\/jkpgye-36.myshopify.com\/collections\/canine-primary-cells.oembed","provider":"Cell Biologics","version":"1.0","type":"link"}