{"title":"Bone-marrow","description":"","products":[{"product_id":"aged-mouse-bone-marrow-neutrophils","title":"Aged Mouse Bone Marrow Neutrophils","description":"\u003cp\u003eAged Mouse Bone Marrow Neutrophils - Aged Mouse primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527518572828,"sku":"A57-6029","price":637.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687243501852,"sku":"A57-6029","price":850.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687243534620,"sku":"A57-6029","price":1062.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-7c89b4d443.jpg?v=1791766977"},{"product_id":"aged-mouse-bone-marrow-macrophages","title":"Aged Mouse Bone Marrow Macrophages","description":"\u003cp\u003eAged Mouse Bone Marrow Macrophages - Aged Mouse primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (3 x 10^6 cells)","offer_id":54527518605596,"sku":"A57-6030","price":725.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687243567388,"sku":"A57-6030","price":850.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687243600156,"sku":"A57-6030","price":1062.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-20de0950f8.jpg?v=1791767016"},{"product_id":"aged-mouse-bone-marrow-mesenchymal-stem-cells","title":"Aged Mouse Bone Marrow Mesenchymal Stem Cells","description":"\u003cp\u003eAged Mouse Bone Marrow Mesenchymal Stem Cells - Aged Mouse primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527519097116,"sku":"A57-6043","price":725.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687243632924,"sku":"A57-6043","price":787.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687243665692,"sku":"A57-6043","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-4dd4a9b8a9.jpg?v=1791766978"},{"product_id":"aged-mouse-bone-marrow-dendritic-cells","title":"Aged Mouse Bone Marrow Dendritic Cells","description":"\u003cp\u003eAged Mouse Bone Marrow Dendritic Cells - Aged Mouse primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (2 x 10^6 cells)","offer_id":54527521292572,"sku":"A57-6200","price":725.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-bd1e16ef3f.jpg?v=1791766977"},{"product_id":"aged-mouse-bone-marrow-derived-endothelial-cells","title":"Aged Mouse Bone Marrow-Derived Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eAged Mouse Primary Bone Marrow-derived Endothelial Cells \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. A57-6221\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAgedMouse Primary Bone Marrow-derived Endothelial Cells from Cell Biologics are isolated from bone marrow of 62-78 week old pathogen-free laboratory mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. AgedMouse Primary Bone Marrow-derived Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (AGED44, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAged Mouse Primary Bone Marrow-derived Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527521915164,"sku":"A57-6221","price":725.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687075926300,"sku":"A57-6221","price":787.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687075959068,"sku":"A57-6221","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff5cea3fab.jpg?v=1791767017"},{"product_id":"aged-rat-bone-marrow-macrophages","title":"Aged Rat Bone Marrow Macrophages","description":"\u003cp\u003eAged Rat Bone Marrow Macrophages - Aged Rat primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (3 x 10^6 cells)","offer_id":54527522799900,"sku":"AR-6030","price":580.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687243698460,"sku":"AR-6030","price":680.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687243731228,"sku":"AR-6030","price":850.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-20de0950f8.jpg?v=1791767016"},{"product_id":"b129-mouse-bone-marrow-neutrophils","title":"B129 Mouse Bone Marrow Neutrophils","description":"\u003cp\u003eB129 Mouse Bone Marrow Neutrophils - Mouse primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527525716252,"sku":"B129-7029","price":510.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-7c89b4d443.jpg?v=1791766977"},{"product_id":"b129-mouse-bone-marrow-macrophages-frozen-cells","title":"B129 Mouse Bone Marrow Macrophages (Frozen Cells)","description":"\u003cp\u003e\u003cb\u003eB129 Mouse Bone Marrow Macrophages (Frozen Cells)\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eB129-7030\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Macrophage Medium \/w Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCatalog No. M3368\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eB129 Mouse Bone Marrow Macrophages are isolated from the tibias and femurs of pathogen-free laboratory adult mice. Cells at passage 0 are cryo-preserved in vials. Each vial contains 3x10\u003csup\u003e6\u003c\/sup\u003e cells and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma and tested for expression of markers using antibody, CD11b by flow cytometry. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eB129 Mouse Bone Marrow Macrophages can be used in standard biochemical procedures include PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e will ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use. Live-cell shipment is also available on request. Primary cells can never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eB129 Mouse Bone Marrow Macrophages from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for \u003ci\u003ein vitro\u003c\/i\u003e diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e, without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all Cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (3 x 10^6 cells)","offer_id":54527525749020,"sku":"B129-7030","price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-b937ca7319.jpg?v=1791767016"},{"product_id":"b129-mouse-bone-marrow-mesenchymal-stem-cells","title":"B129 Mouse Bone Marrow Mesenchymal Stem Cells","description":"\u003cp\u003e\u003cstrong\u003eB129 Mouse Bone Marrow Mesenchymal Stem Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. B129-7043\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM5556 Mesenchymal Cell Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eB129 Mouse Bone Marrow Mesenchymal Stem Cells from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory B129 mice and grown in tissue culture flasks with Cell Biologics’ Complete Growth Medium. Cells at passage 1-2 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. B129 Mouse Bone Marrow Mesenchymal Stem Cells are characterized by immunofluorescence staining with antibody of CD44, Sca-1 or CD29. These cells can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eB129 Mouse Bone Marrow Mesenchymal Stem Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527526109468,"sku":"B129-7043","price":580.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687245893916,"sku":"B129-7043","price":630.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687245926684,"sku":"B129-7043","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-1e5002f3cf.jpg?v=1791766977"},{"product_id":"b129-mouse-bone-marrow-dendritic-cells","title":"B129 Mouse Bone Marrow Dendritic Cells","description":"\u003cp\u003e\u003cstrong\u003eB129 Mouse Bone Marrow Dendritic Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. B129-7200\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM7711 Complete Dendritic Cell Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eB129 Mouse Bone Marrow Dendritic Cells from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory B129 mice. Cells at passage 0 are cryo-preserved and each vial contains 2x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. B129 Mouse Bone Marrow Dendritic Cells are characterized by immunofluorescence staining with antibody of CD11c. These cells will not proliferate in culture and cannot be passaged.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eB129 Mouse Bone Marrow Dendritic Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e_____________________________________________________________________________________\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium:\u003c\/strong\u003e Use pre-warmed (37°C) cell culture media of Catalog No. M7711 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell recovery from cryovial:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (2 x 10^6 cells)","offer_id":54527527747868,"sku":"B129-7200","price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-bd1e16ef3f.jpg?v=1791766977"},{"product_id":"b129-mouse-primary-bone-marrow-derived-endothelial-cells","title":"B129 Mouse Primary Bone Marrow-Derived Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eB129 Mouse Primary Bone Marrow-Derived Endothelial Cells \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. B129-7221\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eB129 Mouse Primary Bone Marrow-Derived Endothelial Cells from Cell Biologics are isolated from bone marrow of B129S2\/SvPasCrl pathogen-free laboratory mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. B129 Mouse Primary Bone Marrow-Derived Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (B12944, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eB129 Mouse Primary Bone Marrow-Derived Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527528304924,"sku":"B129-7221","price":580.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687249236252,"sku":"B129-7221","price":630.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687249269020,"sku":"B129-7221","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-63d14ba0ac.jpg?v=1791767087"},{"product_id":"b129-mouse-bone-marrow-monocytes","title":"B129 Mouse Bone Marrow Monocytes","description":"\u003cp\u003e\u003cstrong\u003eB129 Mouse Bone Marrow Monocytes \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. B129-7271F\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM3366 Complete Leukocyte Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eB129 Mouse Bone Marrow Monocytes from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory B129 mice. Cells at passage 0 are cryo-preserved and each vial contains 3 x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. B129 Mouse Bone Marrow Monocytes can be used for designed experiments under the cell culture conditions specified by Cell Biologics. These cells will not proliferate in culture and cannot be passaged.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eB129 Mouse Bone Marrow Monocytes from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e_____________________________________________________________________________________\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium:\u003c\/strong\u003e Use pre-warmed (37°C) cell culture media of Catalog No. M7711 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell recovery from cryovial:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527528403228,"sku":"B129-7271","price":760.0,"currency_code":"USD","in_stock":true},{"title":"Frozen Vial 3 x 10^6","offer_id":54687249301788,"sku":"B129-7271","price":410.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-34b0a0790d.jpg?v=1791766978"},{"product_id":"balb-c-mouse-bone-marrow-neutrophils-frozen-cells","title":"BALB\/c Mouse Bone Marrow Neutrophils (Frozen Cells)","description":"\u003cp\u003e\u003cstrong\u003eBALB\/c Mouse Bone Marrow Neutrophils \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. BALB-5029F\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM3367 Complete Neutrophil Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Bone Marrow Neutrophils from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory BALB\/c mice. The cells at passage 0 are cryo-preserved, each vial contains 5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. These cells can be used for designed experiments under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Bone Marrow Neutrophils from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e_______________________________________________________________________________________\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003eMedium: Use pre-warmed (37°C) cell culture media of Catalog No. M3367 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003eCell recovery from cryovial:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527529353500,"sku":"BALB-5029","price":510.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-74b86a7a2f.jpg?v=1791767016"},{"product_id":"balb-c-mouse-bone-marrow-macrophages-frozen-cells","title":"BALB\/c Mouse Bone Marrow Macrophages (Frozen Cells)","description":"\u003cp\u003e\u003cstrong\u003eBALB\/c Mouse Bone Marrow Macrophages\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. BALB-5030F\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM3368 Complete Macrophage Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Bone Marrow Macrophages from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory BALB\/c mice and grown in tissue culture plates with Cell Biologics’ Cell Culture Medium. Cells at passage 0 are cryo-preserved and each vial contains 3x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. BALB\/c Mouse Bone Marrow Macrophages are characterized by immunofluorescence staining using antibody of CD11b or F4\/80. These cells can be plated on multiwell culture plates for experiments under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Bone Marrow Macrophages from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e________________________________________________________________________________________\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Macrophage Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a bio-safety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eReview the information provided on the Cell Biologics website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture media (30-50 ML, Catalog No. M3368) to seed cells and when changing media.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCoating of Cell Culture Plates or Dishes\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCoat sterile culture dishes or flasks with Gelatin Coating Solution (Catalog No. 6950, Cell Biologics) for 2 min, then aspirate the excess solution.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell Recovery from Cryovial\u003c\/strong\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003e Thaw the cells quickly (\u0026lt;2 min) in a 37°C water bath until just prior to complete thawing.\u003c\/li\u003e\n\u003cli\u003e Wipe the outside of the vial with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Use 1 ml Pipette Tip to resuspend cells and transfer cells to a 15 ml sterile tube.\u003c\/li\u003e\n\u003cli\u003e Add 5 ml of pre-warmed Cell Biologics’ Cell Culture Medium (Catalog number M3368, Cell Biologics) gently to the side of the tube and slowly pipette up and down 2 to 3 times to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003e Centrifuge the cells at 200 x g at 4°C for 5 min.\u003c\/li\u003e\n\u003cli\u003e Carefully remove the supernatant and resuspend in 7 mL pre-warmed Maintenance Medium (Catalog No. M3368, Cell Biologics).\u003c\/li\u003e\n\u003cli\u003e Carefully pour cell suspension into the cell culture plate or dish.\u003c\/li\u003e\n\u003cli\u003e Recommended Cell Seeding: 0.7-0.8 million cells are seeded per well of a 12-well plate or 1-1.5 million macroPlace a plate in a humidified, 5%-CO\u003csub\u003e2\u003c\/sub\u003e incubator at 37°C until experiments.\u003c\/li\u003e\n\u003cli\u003e Change fresh cell culture medium every 24-48 hours.\u003c\/li\u003e\n\u003cli\u003e Cells should be checked daily under a microscope to verify appropriate cell morphology.\u003c\/li\u003e\n\u003cli\u003e Proceed with experiment assays.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cp\u003eNote: Please send us the cell images (\u0026gt;90% confluence) if you have any question or problem with cultured cells.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (3 x 10^6 cells)","offer_id":54527529386268,"sku":"BALB-5030","price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-36cf55ce90.jpg?v=1791767054"},{"product_id":"balb-c-mouse-bone-marrow-mesenchymal-stem-cells","title":"BALB\/c Mouse Bone Marrow Mesenchymal Stem Cells","description":"\u003cp\u003e\u003cstrong\u003eBALB\/c Mouse Bone Marrow Mesenchymal Stem Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. BALB-5043\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM5556 Mesenchymal Cell Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Bone Marrow Mesenchymal Stem Cells from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory BALB\/c mice and grown in tissue culture flasks with Cell Biologics’ Complete Growth Medium. Cells at passage 1-2 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. BALB\/c Mouse Bone Marrow Mesenchymal Stem Cells are characterized by immunofluorescence staining with antibody of CD44, Sca-1 or CD29. These cells can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Bone Marrow Mesenchymal Stem Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003ePublication:\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10340256\/\" target=\"_blank\" rel=\"noopener\"\u003eCells.\u003c\/a\u003e 2023 Jul; 12(13): 1771.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527529746716,"sku":"BALB-5043","price":580.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687250972956,"sku":"BALB-5043","price":630.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687251005724,"sku":"BALB-5043","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-0049e31f1e.jpg?v=1791767054"},{"product_id":"balb-c-mouse-bone-marrow-dendritic-cells","title":"BALB\/c Mouse Bone Marrow Dendritic Cells","description":"\u003cp\u003e\u003cstrong\u003eBALB\/c Mouse Bone Marrow Dendritic Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. BALB-5200\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM7711 Complete Dendritic Cell Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Bone Marrow Dendritic Cells from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory BALB\/c mice. Cells at passage 0 are cryo-preserved and each vial contains 1x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. BALB\/c Mouse Bone Marrow Dendritic Cells are characterized by immunofluorescence staining with antibody of CD11c. These cells will not proliferate in culture and cannot be passaged.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Bone Marrow Dendritic Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e_____________________________________________________________________________________\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium:\u003c\/strong\u003e Use pre-warmed (37°C) cell culture media of Catalog No. M7711 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell recovery from cryovial:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (2 x 10^6 cells)","offer_id":54527531909404,"sku":"BALB-5200","price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-b9e283e53d.jpg?v=1791767087"},{"product_id":"balb-c-mouse-primary-bone-marrow-derived-endothelial-cells","title":"BALB\/c Mouse Primary Bone Marrow-Derived Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eBALB\/c Mouse Primary Bone Marrow-Derived Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. BALB-5221\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Primary Bone Marrow-derived Endothelial Cells from Cell Biologics are isolated from bone marrow of pathogen-free laboratory mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. BALB\/c Mouse Primary Bone Marrow-derived Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Primary Bone Marrow-derived Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003e\u003cstrong\u003eCell Culture Protocol\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527532728604,"sku":"BALB-5221","price":580.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687255068956,"sku":"BALB-5221","price":630.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687255101724,"sku":"BALB-5221","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-b220947dc7.jpg?v=1791767016"},{"product_id":"balb-c-mouse-bone-marrow-monocytes","title":"BALB\/c Mouse Bone Marrow Monocytes","description":"\u003cp\u003e\u003cstrong\u003eBALB\/c Mouse Bone Marrow Monocytes \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. BALB-5271F\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM3366 Complete Leukocyte Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Bone Marrow Monocytes from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory BALB\/c mice. Cells at passage 0 are cryo-preserved and each vial contains 3 x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. BALB\/c Mouse Bone Marrow Monocytes can be used for designed experiments under the cell culture conditions specified by Cell Biologics. These cells will not proliferate in culture and cannot be passaged.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eBALB\/c Mouse Bone Marrow Monocytes from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e_____________________________________________________________________________________\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium:\u003c\/strong\u003e Use pre-warmed (37°C) cell culture media of Catalog No. M7711 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell recovery from cryovial:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527533154588,"sku":"BALB-5271","price":760.0,"currency_code":"USD","in_stock":true},{"title":"Frozen Vial 3 x 10^6","offer_id":54687315624220,"sku":"BALB-5271","price":410.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-c3c8a4aaed.jpg?v=1791766977"},{"product_id":"c57bl-6-mouse-bone-marrow-neutrophils","title":"C57BL\/6 Mouse Bone Marrow Neutrophils","description":"\u003cp\u003e\u003cstrong\u003eC57BL\/6 Mouse Bone Marrow Neutrophils \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6029F\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM3366 Complete Leukocyte Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Bone Marrow Neutrophils from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory adult mice. Cells at passage 0 are cryo-preserved and each vial contains 5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. Cells in suspension culturecan be used for designed experiments under the cell culture conditions specified by Cell Biologics. Cells can be used in standard biochemical procedures include FACS, PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications. Cells are negative for bacteria, yeast, fungi, and mycoplasma. These cells will not proliferate in culture and cannot be passaged.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Bone Marrow Neutrophils from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e_____________________________________________________________________________________\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium:\u003c\/strong\u003e Use pre-warmed (37°C) cell culture media of Catalog No. M7711 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell recovery from cryovial:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527540396316,"sku":"C57-6029","price":510.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-7c89b4d443.jpg?v=1791766977"},{"product_id":"c57bl-6-mouse-bone-marrow-macrophages-frozen-cells","title":"C57BL\/6 Mouse Bone Marrow Macrophages (Frozen Cells)","description":"\u003cstrong\u003eC57BL\/6 Mouse Bone Marrow Macrophages\u003c\/strong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003eCatalog No. C57-6030F\u003c\/strong\u003e\u003cbr\u003e Suggested Medium M3368 Complete Macrophage Medium w\/ Kit (500 ml) \u003cp\u003e\u003cstrong\u003eProduct Description \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Bone Marrow Macrophages from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory C57BL\/6 mice and grown in tissue culture plates with Cell Biologics’ Cell Culture Medium. Cells at passage 0 are cryo-preserved and each vial contains 3x10\u003csup\u003e6 \u003c\/sup\u003ecells per ml. C57BL\/6 Mouse Bone Marrow Macrophages are characterized by immunofluorescence staining using antibody of CD11b or F4\/80. These cells can be plated on multiwell culture plates for experiments under the cell culture conditions specified by Cell Biologics. Cells can not be cultured indefinitely and can not be passaged. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Bone Marrow Macrophages from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order. \u003c\/p\u003e\u003cp\u003eDisclaimer Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. Warranty and Liability \u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Macrophage Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a bio-safety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eMedium Review the information provided on the Cell Biologics website about appropriate culture media (e.g. serum and other supplements).\u003c\/p\u003e\u003cp\u003eUse pre-warmed (37°C) cell culture media (30-50 ML, Catalog No. M3368) to seed cells and when changing media.\u003c\/p\u003e\u003cp\u003eCoating of Cell Culture Plates or Dishes Coat sterile culture dishes or flasks with Gelatin Coating Solution (Catalog No. 6950, Cell Biologics) for 2 min, then aspirate the excess solution.\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell Recovery from Cryovial \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eThaw the cells quickly (\u0026lt;2 min) in a 37°C water bath until just prior to complete thawing.\u003c\/p\u003e\u003cp\u003eWipe the outside of the vial with 70% ethanol.\u003c\/p\u003e\u003cp\u003eUse 1 ml Pipette Tip to resuspend cells and transfer cells to a 15 ml sterile tube.\u003c\/p\u003e\u003cp\u003eAdd 5 ml of pre-warmed Cell Biologics’ Cell Culture Medium (Catalog number M3368, Cell Biologics) gently to the side of the tube and slowly pipette up and down 2 to 3 times to resuspend the cells.\u003c\/p\u003e\u003cp\u003eCentrifuge the cells at 200 x g at 4°C for 5 min.\u003c\/p\u003e\u003cp\u003eCarefully remove the supernatant and resuspend in 7 mL pre-warmed Maintenance Medium (Catalog No. M3368, Cell Biologics).\u003c\/p\u003e\u003cp\u003eCarefully pour cell suspension into the cell culture plate or dish.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRecommended Cell Seeding: \u003c\/strong\u003e0.7-0.8 million cells are seeded per well of a 12-well plate or 1-1.5 million macroPlace a plate in a humidified, 5%-CO\u003csub\u003e2\u003c\/sub\u003e incubator at 37°C until experiments.\u003c\/p\u003e\u003cp\u003eChange fresh cell culture medium every 24-48 hours. Cells should be checked daily under a microscope to verify appropriate cell morphology.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProceed with experiment assays.\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eNote: Please send us the cell images (\u0026gt;90% confluence) if you have any question or problem with cultured cells.\u003c\/p\u003e\u003cp\u003ePublication:\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8263005\/\" target=\"_blank\" rel=\"noopener\"\u003emBio.\u003c\/a\u003e 2021 May-Jun; 12(3): e01227-21.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8769200\/\" target=\"_blank\" rel=\"noopener\"\u003emSphere.\u003c\/a\u003e 2022 Jan-Feb; 7(1): e00934-21.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (3 x 10^6 cells)","offer_id":54527540494620,"sku":"C57-6030","price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-5c584d3d0f.jpg?v=1791766977"},{"product_id":"gfp-expressing-c57bl-6-mouse-bone-marrow-macrophages","title":"GFP-Expressing C57BL\/6 Mouse Bone Marrow Macrophages","description":"GFP Expressing C57BL\/6 Mouse Bone Marrow Macrophages\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527540527388,"sku":"C57-6030GFP","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-5c584d3d0f.jpg?v=1791766977"},{"product_id":"rfp-expressing-c57bl-6-mouse-bone-marrow-macrophages","title":"RFP-Expressing C57BL\/6 Mouse Bone Marrow Macrophages","description":"RFP Expressing C57BL\/6 Mouse Bone Marrow Macrophages\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527540560156,"sku":"C57-6030RFP","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-5c584d3d0f.jpg?v=1791766977"},{"product_id":"c57bl-6-mouse-bone-marrow-mesenchymal-stem-cells-b6msc","title":"C57BL\/6 Mouse Bone Marrow Mesenchymal Stem Cells (B6MSC)","description":"\u003cp\u003e\u003cstrong\u003eC57BL\/6 Mouse Bone Marrow Mesenchymal Stem Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6043\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM5566 Mesenchymal Cell Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Bone Marrow Mesenchymal Stem Cells from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory C57BL\/6 mice and grown in tissue culture flasks with Cell Biologics’ Complete Growth Medium. Cells at passage 1-2 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. C57BL\/6 Mouse Bone Marrow Mesenchymal Stem Cells are characterized by immunofluorescence staining with antibody of CD44, Sca-1 or CD29. These cells can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Bone Marrow Mesenchymal Stem Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003ePublication:\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC9847523\/\" target=\"_blank\" rel=\"noopener\"\u003eRegen Biomater.\u003c\/a\u003e 2023; 10: rbac084.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527544525084,"sku":"C57-6043","price":580.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687315689756,"sku":"C57-6043","price":630.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-4dd4a9b8a9.jpg?v=1791766978"},{"product_id":"cas9-expressing-c57bl-6-mouse-bone-marrow-mesenchymal-stem-cells","title":"Cas9-Expressing C57BL\/6 Mouse Bone Marrow Mesenchymal Stem Cells","description":"Cas9 Expressing C57BL\/6 Mouse Bone Marrow Mesenchymal Stem Cells\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527544557852,"sku":"C57-6043Cas9","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-4dd4a9b8a9.jpg?v=1791766978"},{"product_id":"cre-expressing-mouse-bone-marrow-mesenchymal-stem-cells-b6msc","title":"Cre-Expressing Mouse Bone Marrow Mesenchymal Stem Cells (B6MSC)","description":"\u003cp\u003eCre-Expressing Mouse Bone Marrow Mesenchymal Stem Cells (B6MSC) - Mouse primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527544590620,"sku":"C57-6043Cre","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-4dd4a9b8a9.jpg?v=1791766978"},{"product_id":"gfp-expressing-c57bl-6-mouse-bone-marrow-mesenchymal-stem-cells","title":"GFP-Expressing C57BL\/6 Mouse Bone Marrow Mesenchymal Stem Cells","description":"GFP Expressing C57BL\/6 Mouse Bone Marrow Mesenchymal Stem Cells\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527544623388,"sku":"C57-6043GFP","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-4dd4a9b8a9.jpg?v=1791766978"},{"product_id":"immortalized-mouse-bone-marrow-mesenchymal-stem-cells","title":"Immortalized Mouse Bone Marrow Mesenchymal Stem Cells","description":"Immortalized Mouse Bone Marrow Mesenchymal Stem Cells\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527544656156,"sku":"C57-6043IM","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-4dd4a9b8a9.jpg?v=1791766978"},{"product_id":"immortalized-gfp-expressing-mouse-bone-marrow-mesenchymal-stem-cells","title":"Immortalized GFP-Expressing Mouse Bone Marrow Mesenchymal Stem Cells","description":"Immortalized GFP-Expressing Mouse Bone Marrow Mesenchymal Stem Cells\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527544688924,"sku":"C57-6043IM.GFP","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-4dd4a9b8a9.jpg?v=1791766978"},{"product_id":"immortalized-rfp-expressing-mouse-bone-marrow-mesenchymal-stem-cells","title":"Immortalized RFP-Expressing Mouse Bone Marrow Mesenchymal Stem Cells","description":"\u003cp\u003eImmortalized RFP-Expressing Mouse Bone Marrow Mesenchymal Stem Cells - Mouse primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527544721692,"sku":"C57-6043IM.RFP","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-4dd4a9b8a9.jpg?v=1791766978"},{"product_id":"luciferase-expressing-c57bl-6-mouse-bone-marrow-mesenchymal-stem-cells","title":"Luciferase-Expressing C57BL\/6 Mouse Bone Marrow Mesenchymal Stem Cells","description":"\u003cp\u003e\u003cstrong\u003eLuciferase-Expressing C57BL\/6 Mouse Bone Marrow Mesenchymal Stem Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6043Luc\u003c\/p\u003e\u003cp\u003eSuggested Medium\u003c\/p\u003e\u003cp\u003eM5566 Mesenchymal Cell Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003eProduct Description\u003c\/p\u003e\u003cp\u003eLuciferase-Expressing Mouse Bone Marrow Mesenchymal Stem Cells from Cell Biologics are isolated from the tibias and femurs of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Luciferase-Expressing Mouse Primary Bone Marrow Mesenchymal Stem Cells are characterized by immunofluorescence staining with antibody of CD44, Sca-1 or CD29. These cells can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eLuciferase, distinct from GFP\/RFP photoprotein, is widely used in bioluminescence. Cell Biologics generates various Firefly luciferase-expressing stable cells, including endothelial cells, epithelial cells and so forth. Firefly luciferase-expressing cells are developed through transducing Firefly luciferase-lentiviral particles.\u003c\/p\u003e\u003cp\u003eStorage\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/p\u003e\u003cp\u003eLuciferase-Expressing Mouse Primary Bone Marrow Mesenchymal Stem Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003eDisclaimer\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003eWarranty and Liability\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527544754460,"sku":"C57-6043Luc","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-fdeff8eb79.jpg?v=1791766977"},{"product_id":"rfp-expressing-c57bl-6-mouse-bone-marrow-mesenchymal-stem-cells","title":"RFP-Expressing C57BL\/6 Mouse Bone Marrow Mesenchymal Stem Cells","description":"RFP Expressing C57BL\/6 Mouse Bone Marrow Mesenchymal Stem Cells\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527544885532,"sku":"C57-6043RFP","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-4dd4a9b8a9.jpg?v=1791766978"},{"product_id":"c57bl-6-mouse-bone-marrow-dendritic-cells","title":"C57BL\/6 Mouse Bone Marrow Dendritic Cells","description":"\u003cp\u003e\u003cstrong\u003eC57BL\/6 Mouse Bone Marrow Dendritic Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6200\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM7711 Complete Dendritic Cell Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Bone Marrow Dendritic Cells from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory C57BL\/6 mice. Cells at passage 0 are cryo-preserved and each vial contains 1x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. C57BL\/6 Mouse Bone Marrow Dendritic Cells are characterized by immunofluorescence staining with antibody of CD11c. These cells will not proliferate in culture and cannot be passaged.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Bone Marrow Dendritic Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e_____________________________________________________________________________________\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium:\u003c\/strong\u003e Use pre-warmed (37°C) cell culture media of Catalog No. M7711 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell recovery from cryovial:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (2 x 10^6 cells)","offer_id":54527562154268,"sku":"C57-6200","price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-b9e283e53d.jpg?v=1791767087"},{"product_id":"c57bl-6-mouse-primary-bone-marrow-derived-endothelial-cells","title":"C57BL\/6 Mouse Primary Bone Marrow-Derived Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eC57BL\/6 Mouse Primary Bone Marrow-derived Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6221\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Primary Bone Marrow-derived Endothelial Cells from Cell Biologics are isolated from Bone Marrow tissues of pathogen-free laboratory mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. C57BL\/6 Mouse Primary Bone Marrow-derived Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended. C57BL\/6 Mouse Primary Bone Marrow-derived Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Primary Bone Marrow-derived Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003e\u003cstrong\u003eCell Culture Protocol\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePublication:\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6821596\/\" target=\"_blank\" rel=\"noopener\"\u003eHaematologica.\u003c\/a\u003e 2019 Nov; 104(11): 2164–2177.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10014525\/\" target=\"_blank\" rel=\"noopener\"\u003eFront Pharmacol.\u003c\/a\u003e 2023; 14: 1125209.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527568314652,"sku":"C57-6221","price":580.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687317229852,"sku":"C57-6221","price":630.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687317262620,"sku":"C57-6221","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-63d14ba0ac.jpg?v=1791767087"},{"product_id":"cas9-expressing-c57bl-6-mouse-primary-bone-marrow-derived-endothelial-cells","title":"Cas9-Expressing C57BL\/6 Mouse Primary Bone Marrow-Derived Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eCas9-Expressing Mouse Primary Bone Marrow-derived Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6221Cas9\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCas9-Expressing Mouse Primary Bone Marrow-derived Endothelial Cells from Cell Biologics are isolated from bone marrow of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Cas9-Expressing Mouse Primary Bone Marrow-derived Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003eCell Biologics generates Cas9-expressing stable cells through transducing Cas9-lentiviral particles. Cas9-stable cells express wild-type Cas endonuclease.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCas9-Expressing Mouse Primary Bone Marrow-derived Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527568347420,"sku":"C57-6221Cas9","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-63d14ba0ac.jpg?v=1791767087"},{"product_id":"cre-expressing-mouse-primary-bone-marrow-derived-endothelial-cells","title":"Cre-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eCre-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6221Cre\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCre-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells from Cell Biologics are isolated from bone marrow of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Cre-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003eCell Biologics generates the Cre-expressing stable cells by transducing Cre recombinase. LOXP constructs are transfected into the stable cells that can be performed various biological analyses.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCre-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527568380188,"sku":"C57-6221Cre","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-63d14ba0ac.jpg?v=1791767087"},{"product_id":"gfp-expressing-c57bl-6-mouse-primary-bone-marrow-derived-endothelial-cells","title":"GFP-Expressing C57BL\/6 Mouse Primary Bone Marrow-Derived Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eGFP-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6221GFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eGFP-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells from Cell Biologics are isolated from bone marrow of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. GFP-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003eThe green fluorescent protein (GFP) gives rise to very bright green fluorescence when exposed to blue light. Cell Biologics generates various GFP-expressing stable cells, including Macrophages, epithelial cells and so forth. GFP-expressing cells are developed through transducing GFP-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eGFP-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527568412956,"sku":"C57-6221GFP","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-63d14ba0ac.jpg?v=1791767087"},{"product_id":"immortalized-mouse-primary-bone-marrow-derived-endothelial-cells","title":"Immortalized Mouse Primary Bone Marrow-Derived Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eImmortalized Mouse Primary Bone Marrow-Derived Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6221IM\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized Mouse Primary Bone Marrow-Derived Endothelial Cells from Cell Biologics are isolated from bone marrow of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Immortalized Mouse Primary Bone Marrow-Derived Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for more than 10 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eImmortalized cell lines from Cell Biologics were derived from primary cells with extended life span. The primary cells were immortalized by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized Mouse Primary Bone Marrow-Derived Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527568445724,"sku":"C57-6221IM","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-63d14ba0ac.jpg?v=1791767087"},{"product_id":"immortalized-gfp-expressing-mouse-primary-bone-marrow-derived-endothelial-cells","title":"Immortalized GFP-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eImmortalized GFP-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6221IM.GFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells from Cell Biologics are isolated from bone marrow of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. \u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for more than 10 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003eCell Biologics generated the immortalized cells by transducing human telomerase reverse transcriptase. The immortalized cells have an extended life span without altering the properties of the origin cells.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527568478492,"sku":"C57-6221IM.GFP","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-63d14ba0ac.jpg?v=1791767087"},{"product_id":"immortalized-rfp-expressing-mouse-primary-bone-marrow-derived-endothelial-cells","title":"Immortalized RFP-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells","description":"\u003cp\u003eImmortalized RFP-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells - Mouse primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527568511260,"sku":"C57-6221IM.RFP","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-63d14ba0ac.jpg?v=1791767087"},{"product_id":"luciferase-expressing-c57bl-6-mouse-primary-bone-marrow-derived-endothelial-cells","title":"Luciferase-Expressing C57BL\/6 Mouse Primary Bone Marrow-Derived Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eLuciferase-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6221Luc\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLuciferase-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells from Cell Biologics are isolated from bone marrow of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 1x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Luciferase-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003eLuciferase, distinct from GFP\/RFP photoprotein, is widely used in bioluminescence. Cell Biologics generates various Firefly luciferase-expressing stable cells, including endothelial cells, epithelial cells and so forth. Firefly luciferase-expressing cells are developed through transducing Firefly luciferase-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eLuciferase-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527568544028,"sku":"C57-6221Luc","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-63d14ba0ac.jpg?v=1791767087"},{"product_id":"rfp-expressing-c57bl-6-mouse-primary-bone-marrow-derived-endothelial-cells","title":"RFP-Expressing C57BL\/6 Mouse Primary Bone Marrow-Derived Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eRFP-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6221RFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRFP-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells from Cell Biologics are isolated from the bone marrow of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. RFP-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eRed Fluorescent Protein (RFP), a mutant form of green fluorescent protein, gives rise to very bright red fluorescence when exposed to yellow-green light. Cell Biologics generates various RFP-expressing stable cells, including endothelial cells, epithelial cells and so forth. RFP-expressing cells are developed through transducing RFP-lentiviral particles.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eRFP-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527568576796,"sku":"C57-6221RFP","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-63d14ba0ac.jpg?v=1791767087"},{"product_id":"c57bl-6-mouse-bone-marrow-monocytes","title":"C57BL\/6 Mouse Bone Marrow Monocytes","description":"\u003cp\u003e\u003cstrong\u003eC57BL\/6 Mouse Bone Marrow Monocytes \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. C57-6271F\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM3366 Complete Leukocyte Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Bone Marrow Monocytes from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory C57BL\/6 mice. Cells at passage 0 are cryo-preserved and each vial contains 3x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. Cells in suspension culturecan be used for designed experiments under the cell culture conditions specified by Cell Biologics. Cells can be used in standard biochemical procedures include FACS, PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications. Cells are negative for bacteria, yeast, fungi, and mycoplasma. These cells will not proliferate in culture and cannot be passaged.\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eC57BL\/6 Mouse Bone Marrow Monocytes from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e_____________________________________________________________________________________\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium:\u003c\/strong\u003e Use pre-warmed (37°C) cell culture media of Catalog No. M7711 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell recovery from cryovial:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003ePublication:\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/entrez\/eutils\/elink.fcgi?dbfrom=pubmed\u0026amp;retmode=ref\u0026amp;cmd=prlinks\u0026amp;id=35705056\" target=\"_blank\" rel=\"noopener\"\u003eCell Rep. 2022 Jun 14; 39(11): 110961.\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527570510108,"sku":"C57-6271","price":510.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-c3c8a4aaed.jpg?v=1791766977"},{"product_id":"cd1-mouse-bone-marrow-neutrophils","title":"CD1 Mouse Bone Marrow Neutrophils","description":"\u003cp\u003e\u003cstrong\u003eCD1 Mouse Bone Marrow Neutrophils \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. CD-1029F\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM3367 Complete Neutrophil Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCD1 Mouse Bone Marrow Neutrophils from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory CD1 mice. The cells at passage 0 are cryo-preserved, each vial contains 5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. These cells can be used for designed experiments under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCD1 Mouse Bone Marrow Neutrophils from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e_______________________________________________________________________________________\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003eMedium: Use pre-warmed (37°C) cell culture media of Catalog No. M3367 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003eCell recovery from cryovial:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527573623068,"sku":"CD-1029","price":510.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-7c89b4d443.jpg?v=1791766977"},{"product_id":"cd1-mouse-bone-marrow-macrophages-frozen-cells","title":"CD1 Mouse Bone Marrow Macrophages (Frozen Cells)","description":"\u003cp\u003e\u003cb\u003eCD1 Mouse Bone Marrow Macrophages (Frozen Cells)\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eCD-1030\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Macrophage Medium \/w Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCatalog No. M3368\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCD1 Mouse Bone Marrow Macrophages are isolated from the tibias and femurs of pathogen-free laboratory adult mice. Cells at passage 0 are cryo-preserved in vials. Each vial contains 3x10\u003csup\u003e6\u003c\/sup\u003e cells and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma and tested for expression of markers using antibody, CD11b by flow cytometry. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eCells can be plated on culture plates for desired experiments under the cell culture conditions specified by Cell Biologics. Primary Bone Marrow Macrophages can not be cultured indefinitely and can not be passaged. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCD1 Mouse Bone Marrow Macrophages can be used in standard biochemical procedures include PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e will ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use. Live-cell shipment is also available on request. Primary cells can never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCD1 Mouse Bone Marrow Macrophages from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for \u003ci\u003ein vitro\u003c\/i\u003e diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e, without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all Cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Macrophage Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a bio-safety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eMedium Review the information provided on the Cell Biologics website about appropriate culture media (e.g. serum and other supplements).\u003c\/p\u003e\u003cp\u003eUse pre-warmed (37°C) cell culture media (30-50 ML, Catalog No. M3368) to seed cells and when changing media.\u003c\/p\u003e\u003cp\u003eCoating of Cell Culture Plates or Dishes Coat sterile culture dishes or flasks with Gelatin Coating Solution (Catalog No. 6950, Cell Biologics) for 2 min, then aspirate the excess solution.\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell Recovery from Cryovial\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eThaw the cells quickly (\u0026lt;2 min) in a 37°C water bath until just prior to complete thawing.\u003c\/p\u003e\u003cp\u003eWipe the outside of the vial with 70% ethanol.\u003c\/p\u003e\u003cp\u003eUse 1 ml Pipette Tip to resuspend cells and transfer cells to a 15 ml sterile tube.\u003c\/p\u003e\u003cp\u003eAdd 5 ml of pre-warmed Cell Biologics’ Cell Culture Medium (Catalog number M3368, Cell Biologics) gently to the side of the tube and slowly pipette up and down 2 to 3 times to resuspend the cells.\u003c\/p\u003e\u003cp\u003eCentrifuge the cells at 200 x g at 4°C for 5 min.\u003c\/p\u003e\u003cp\u003eCarefully remove the supernatant and resuspend in 7 mL pre-warmed Maintenance Medium (Catalog No. M3368, Cell Biologics).\u003c\/p\u003e\u003cp\u003eCarefully pour cell suspension into the cell culture plate or dish.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRecommended Cell Seeding: \u003c\/strong\u003e0.7-0.8 million cells are seeded per well of a 12-well plate or 1-1.5 million macroPlace a plate in a humidified, 5%-CO\u003csub\u003e2\u003c\/sub\u003e incubator at 37°C until experiments.\u003c\/p\u003e\u003cp\u003eChange fresh cell culture medium every 24-48 hours. Cells should be checked daily under a microscope to verify appropriate cell morphology.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProceed with experiment assays.\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eNote: Please send us the cell images (\u0026gt;90% confluence) if you have any question or problem with cultured cells.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (3 x 10^6 cells)","offer_id":54527573655836,"sku":"CD-1030","price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-b937ca7319.jpg?v=1791767016"},{"product_id":"cd-1-mouse-bone-marrow-mesenchymal-stem-cells","title":"CD-1 Mouse Bone Marrow Mesenchymal Stem Cells","description":"\u003cp\u003e\u003cstrong\u003eCD1 Mouse Bone Marrow Mesenchymal Stem Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. CD-1043\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM5556 Mesenchymal Cell Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCD1 Mouse Bone Marrow Mesenchymal Stem Cells from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory CD1 mice and grown in tissue culture flasks with Cell Biologics’ Complete Growth Medium. Cells at passage 1-2 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. CD1 Mouse Bone Marrow Mesenchymal Stem Cells are characterized by immunofluorescence staining with antibody of CD44, Sca-1 or CD29. These cells can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCD1 Mouse Bone Marrow Mesenchymal Stem Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527574114588,"sku":"CD-1043","price":580.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687319916828,"sku":"CD-1043","price":630.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687319949596,"sku":"CD-1043","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-15214f89ae.jpg?v=1791766978"},{"product_id":"cd1-mouse-bone-marrow-dendritic-cells","title":"CD1 Mouse Bone Marrow Dendritic Cells","description":"\u003cp\u003e\u003cstrong\u003eCD1 Mouse Bone Marrow Dendritic Cells\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. CD-1200\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM7711 Complete Dendritic Cell Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCD1 Mouse Bone Marrow Dendritic Cells from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory CD1 mice. Cells at passage 0 are cryo-preserved and each vial contains 2x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. CD1 Mouse Bone Marrow Dendritic Cells are characterized by immunofluorescence staining with antibody of CD11c. These cells will not proliferate in culture and cannot be passaged.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCD1 Mouse Bone Marrow Dendritic Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e_____________________________________________________________________________________\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium:\u003c\/strong\u003e Use pre-warmed (37°C) cell culture media of Catalog No. M7711 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell recovery from cryovial:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (2 x 10^6 cells)","offer_id":54527578505500,"sku":"CD-1200","price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-34b0a0790d.jpg?v=1791766978"},{"product_id":"cd1-mouse-primary-bone-marrow-derived-endothelial-cells","title":"CD1 Mouse Primary Bone Marrow-Derived Endothelial Cells","description":"\u003cp\u003e\u003cstrong\u003eCD1 Mouse Primary Bone Marrow-Derived Endothelial Cells \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. CD-1221\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCD1 Mouse Primary Bone Marrow-Derived Endothelial Cells from Cell Biologics are isolated from the bone marrow of pathogen-free laboratory mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. CD1 Mouse Primary Bone Marrow-Derived Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCD1 Mouse Primary Bone Marrow-Derived Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol \u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527579259164,"sku":"CD-1221","price":580.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687324471580,"sku":"CD-1221","price":630.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687324504348,"sku":"CD-1221","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-63d14ba0ac.jpg?v=1791767087"},{"product_id":"cd1-mouse-bone-marrow-monocytes","title":"CD1 Mouse Bone Marrow Monocytes","description":"\u003cp\u003e\u003cstrong\u003eCD1 Mouse Bone Marrow Monocytes \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. CD-1271F\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM3366 Complete Leukocyte Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCD1 Mouse Bone Marrow Monocytes from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory CD1 mice. Cells at passage 0 are cryo-preserved and each vial contains 3 x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. CD1 Mouse Bone Marrow Monocytes can be used for designed experiments under the cell culture conditions specified by Cell Biologics. These cells will not proliferate in culture and cannot be passaged.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCD1 Mouse Bone Marrow Monocytes from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e_____________________________________________________________________________________\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Cell Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a biosafety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMedium:\u003c\/strong\u003e Use pre-warmed (37°C) cell culture media of Catalog No. M7711 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell recovery from cryovial:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/li\u003e\n\u003cli\u003e Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/li\u003e\n\u003cli\u003e Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/li\u003e\n\u003cli\u003e Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/li\u003e\n\u003cli\u003e Centrifuge cells at 200 x g for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.\u003c\/li\u003e\n\u003cli\u003e Proceed with designed experiments.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (5 x 10^6 cells)","offer_id":54527579783452,"sku":"CD-1271","price":510.0,"currency_code":"USD","in_stock":true},{"title":"Frozen Vial 3 x 10^6","offer_id":54687324897564,"sku":"CD-1271","price":410.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-c3c8a4aaed.jpg?v=1791766977"},{"product_id":"canine-bone-marrow-macrophages-frozen-cells","title":"Canine Bone Marrow Macrophages (Frozen Cells)","description":"\u003cp\u003e\u003cb\u003eCanine Bone Marrow Macrophages (Frozen Cells)\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003eD-6030\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: Macrophage Medium \/w Kit (500 ml) \u003c\/p\u003e\u003cp\u003e\u003cb\u003eCatalog No. M6030\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCanine Bone Marrow Macrophages are derived from the tibias or femurs of Beagle dog. Cells at passage 0 are cryo-preserved in vials. Each vial contains 3x10\u003csup\u003e6\u003c\/sup\u003e cells and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma and tested for expression of markers using antibody, CD11b by flow cytometry. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003ePrimary Bone Marrow Macrophages can not be cultured indefinitely and can not be passaged. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCanine Bone Marrow Macrophages can be used in standard biochemical procedures include PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e will ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use. Live-cell shipment is also available on request. Primary cells can never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCanine Bone Marrow Macrophages from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e, without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all Cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePrimary Macrophage Culture Protocol\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAll cell culture procedures must be conducted in a bio-safety cabinet.\u003c\/p\u003e\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\u003cp\u003eMedium Review the information provided on the Cell Biologics website about appropriate culture media (e.g. serum and other supplements).\u003c\/p\u003e\u003cp\u003eUse pre-warmed (37°C) cell culture media (30-50 ML, Catalog No. M3368) to seed cells and when changing media.\u003c\/p\u003e\u003cp\u003eCoating of Cell Culture Plates or Dishes Coat sterile culture dishes or flasks with Gelatin Coating Solution (Catalog No. 6950, Cell Biologics) for 2 min, then aspirate the excess solution.\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell Recovery from Cryovial\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eThaw the cells quickly (\u0026lt;2 min) in a 37°C water bath until just prior to complete thawing.\u003c\/p\u003e\u003cp\u003eWipe the outside of the vial with 70% ethanol.\u003c\/p\u003e\u003cp\u003eUse 1 ml Pipette Tip to resuspend cells and transfer cells to a 15 ml sterile tube.\u003c\/p\u003e\u003cp\u003eAdd 5 ml of pre-warmed Cell Biologics’ Cell Culture Medium (Catalog number M3368, Cell Biologics) gently to the side of the tube and slowly pipette up and down 2 to 3 times to resuspend the cells.\u003c\/p\u003e\u003cp\u003eCentrifuge the cells at 200 x g at 4°C for 5 min.\u003c\/p\u003e\u003cp\u003eCarefully remove the supernatant and resuspend in 7 mL pre-warmed Maintenance Medium (Catalog No. M3368, Cell Biologics).\u003c\/p\u003e\u003cp\u003eCarefully pour cell suspension into the cell culture plate or dish.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRecommended Cell Seeding: \u003c\/strong\u003e0.7-0.8 million cells are seeded per well of a 12-well plate or 1-1.5 million macroPlace a plate in a humidified, 5%-CO\u003csub\u003e2\u003c\/sub\u003e incubator at 37°C until experiments.\u003c\/p\u003e\u003cp\u003eChange fresh cell culture medium every 24-48 hours. Cells should be checked daily under a microscope to verify appropriate cell morphology.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProceed with experiment assays.\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eNote: Please send us the cell images (\u0026gt;90% confluence) if you have any question or problem with cultured cells.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (3 x 10^6 cells)","offer_id":54527587221788,"sku":"D-6030","price":680.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-5c584d3d0f.jpg?v=1791766977"},{"product_id":"canine-bone-marrow-mesenchymal-stem-cells-dmscs","title":"Canine Bone Marrow Mesenchymal Stem Cells (DMSCs)","description":"\u003cp\u003eCanine Bone Marrow Mesenchymal Stem Cells (DMSCs) - Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527590924572,"sku":"D-6043","price":650.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-4dd4a9b8a9.jpg?v=1791766978"}],"url":"https:\/\/jkpgye-36.myshopify.com\/collections\/bone-marrow\/rabbit.oembed","provider":"Cell Biologics","version":"1.0","type":"link"}