{"title":"BKS db\/db Mouse Cells","description":"\u003cp\u003eBKS db\/db Mouse Cells from Cell Biologics, isolated and quality-tested in our ISO 9001 laboratory for research use.\u003c\/p\u003e","products":[{"product_id":"bks-db-mouse-colonic-smooth-muscle-cells","title":"BKS db Mouse Colonic Smooth Muscle Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Colonic Smooth Muscle Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6001\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m2268\"\u003eSuggested Medium: Smooth Muscle Cell Medium \/w Kit –500 ML\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m2268\"\u003e\u003cb\u003eM2268\u003c\/b\u003e\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Colonic Muscle Cells are isolated from the colonic of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Colonic Smooth Muscle Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics\u003c\/i\u003e’ Culture Complete Growth Medium generally for 3-7 days. Prior to shipping, cells at passage 1 are detached from the culture flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Colonic Muscle Cells from \u003ci\u003eCell Biologics\u003c\/i\u003eare characterized by immunofluorescent method with antibodies to α-smooth muscle actin. BKS db Mouse Colonic Smooth Muscle Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments (or cells may be expanded for 2-4 passages at a split ratio of 1:2) under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics'\u003c\/i\u003e Cell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics \u003c\/i\u003ehas established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics'\u003c\/i\u003e cell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Colonic Muscle Cells can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Colonic Muscle Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527629492508,"sku":"db-6001","price":725.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687823233308,"sku":"db-6001","price":787.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687823266076,"sku":"db-6001","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-54bed8ead2.jpg?v=1791767087"},{"product_id":"bks-db-mouse-artery-endothelial-cells","title":"BKS db Mouse Artery Endothelial cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Artery Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6008\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m1168\"\u003eSuggested Medium: Endothelial Cell Medium \/w Kit – 500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m1168\"\u003e\u003cb\u003eM1168\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Artery Endothelial Cells are isolated from the artery of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Artery Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Artery Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e' Cell Genotype Promise\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003cem\u003eCell Biologics' \u003c\/em\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Artery Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Artery Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527629558044,"sku":"db-6008","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687823298844,"sku":"db-6008","price":875.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687823331612,"sku":"db-6008","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff9dacf44f.jpg?v=1791766977"},{"product_id":"bks-db-mouse-vein-endothelial-cells","title":"BKS db Mouse Vein Endothelial cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Vein Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6009\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m1168\"\u003eSuggested Medium: Endothelial Cell Medium \/w Kit – 500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m1168\"\u003e\u003cb\u003eM1168\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Vein Endothelial Cells are isolated from the vein of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Vein Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Vein Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e' Cell Genotype Promise\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003cem\u003eCell Biologics' \u003c\/em\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mammary Vein Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Vein Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527629590812,"sku":"db-6009","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687823364380,"sku":"db-6009","price":875.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687823397148,"sku":"db-6009","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff9dacf44f.jpg?v=1791766977"},{"product_id":"bks-db-mouse-lung-microvascular-endothelial-cells","title":"BKS db Mouse Lung Microvascular Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Lung Microvascular Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6011\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m1168\"\u003eSuggested Medium: Endothelial Cell Medium \/w Kit – 500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m1168\"\u003e\u003cb\u003eM1168\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Lung Microvascular Endothelial Cells are isolated from the Lung Microvascular of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Lung Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Lung Microvascular Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e' Cell Genotype Promise\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003cem\u003eCell Biologics' \u003c\/em\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Lung Microvascular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Lung Microvascular Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527629656348,"sku":"db-6011","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687823429916,"sku":"db-6011","price":875.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687823462684,"sku":"db-6011","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff9dacf44f.jpg?v=1791766977"},{"product_id":"bks-db-mouse-lung-fibroblasts","title":"BKS db Mouse Lung Fibroblasts","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Lung Fibroblasts\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6013\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m2267\"\u003eSuggested Medium: Fibroblast Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m2267\"\u003e\u003cb\u003eM2267\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Lung Fibroblasts are isolated from the lung of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Lung Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Lung Fibroblasts are tested for expression of marker using the antibody of anti-FSP1\/S100A4 (Millipore USA) by immunofluorescence staining. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics'\u003c\/i\u003e Cell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics'\u003c\/i\u003e cell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Lung Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Lung Fibroblasts from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’ \u003c\/i\u003eonline protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527629721884,"sku":"db-6013","price":637.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687823495452,"sku":"db-6013","price":637.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687823528220,"sku":"db-6013","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-fdeff8eb79.jpg?v=1791766977"},{"product_id":"bks-db-mouse-kidney-endothelial-cells","title":"BKS db Mouse Kidney Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Kidney Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6014\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m1168\"\u003eSuggested Medium: Endothelial Cell Medium \/w Kit – 500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m1168\"\u003e\u003cb\u003eM1168\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Kidney Endothelial Cells are isolated from the kidney of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Kidney Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Kidney Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e' Cell Genotype Promise\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003cem\u003eCell Biologics' \u003c\/em\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Kidney Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Kidney Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527629787420,"sku":"db-6014","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687823593756,"sku":"db-6014","price":875.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687823626524,"sku":"db-6014","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff9dacf44f.jpg?v=1791766977"},{"product_id":"bks-db-mouse-kidney-glomerular-endothelial-cells","title":"BKS db Mouse Kidney Glomerular Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Kidney Glomerular Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6014G\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m1168\"\u003eSuggested Medium: Endothelial Cell Medium \/w Kit – 500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m1168\"\u003e\u003cb\u003eM1168\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Kidney Glomerular Endothelial Cells are isolated from the kidney glomerular of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Kidney Glomerular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Kidney Glomerular Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e' Cell Genotype Promise\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003cem\u003eCell Biologics' \u003c\/em\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Kidney Glomerular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Kidney Glomerular Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527629852956,"sku":"db-6014G","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687823659292,"sku":"db-6014G","price":875.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687823692060,"sku":"db-6014G","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff9dacf44f.jpg?v=1791766977"},{"product_id":"bks-db-mouse-proximal-tubular-epithelial-cells","title":"BKS db Mouse Proximal Tubular Epithelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Proximal Tubular\u003c\/b\u003e\u003cb\u003e Epithelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6015\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=m6621\"\u003eEpithelial Cell Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m6621\"\u003e\u003cb\u003eM6621\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Proximal Tubular Epithelial Cells are isolated from the proximal tubular of mouse homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Proximal Tubular Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Proximal Tubular Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology) or Supernatant (T1 alpha). T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics' \u003c\/i\u003eCell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics' \u003c\/i\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Proximal Tubular Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Proximal Tubular Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent.\u003ci\u003e Cell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics \u003c\/i\u003ewith caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527630049564,"sku":"db-6015","price":775.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687823724828,"sku":"db-6015","price":837.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687823757596,"sku":"db-6015","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-bf490624fc.jpg?v=1791766977"},{"product_id":"bks-db-mouse-kidney-fibroblasts","title":"BKS db Mouse Kidney Fibroblasts","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Kidney Fibroblasts\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6016\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m2267\"\u003eSuggested Medium: Fibroblast Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m2267\"\u003e\u003cb\u003eM2267\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Kidney Fibroblasts are isolated from the kidney of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Kidney Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Kidney Fibroblasts are tested for expression of marker using the antibody of anti-FSP1\/S100A4 (Millipore USA) by immunofluorescence staining. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics'\u003c\/i\u003e Cell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics'\u003c\/i\u003e cell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Kidney Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Kidney Fibroblasts from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’ \u003c\/i\u003eonline protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527630115100,"sku":"db-6016","price":637.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687823790364,"sku":"db-6016","price":637.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687823823132,"sku":"db-6016","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-fdeff8eb79.jpg?v=1791766977"},{"product_id":"bks-db-mouse-liver-sinusoidal-endothelial-cells","title":"BKS db Mouse Liver Sinusoidal Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Liver Sinusoidal Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6017\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m1168\"\u003eSuggested Medium: Endothelial Cell Medium \/w Kit – 500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m1168\"\u003e\u003cb\u003eM1168\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Liver Sinusoidal Endothelial Cells are isolated from the liver of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Liver Sinusoidal Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Liver Sinusoidal Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e' Cell Genotype Promise\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003cem\u003eCell Biologics' \u003c\/em\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Liver Sinusoidal Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Liver Sinusoidal Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527630213404,"sku":"db-6017","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687823855900,"sku":"db-6017","price":875.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687823888668,"sku":"db-6017","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff9dacf44f.jpg?v=1791766977"},{"product_id":"bks-db-mouse-liver-fibroblasts","title":"BKS db Mouse Liver Fibroblasts","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Liver Fibroblasts\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6019\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m2267\"\u003eSuggested Medium: Fibroblast Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m2267\"\u003e\u003cb\u003eM2267\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Liver Fibroblasts are isolated from the liver of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Liver Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Liver Fibroblasts are tested for expression of marker using the antibody of anti-FSP1\/S100A4 (Millipore USA) by immunofluorescence staining. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics'\u003c\/i\u003e Cell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics'\u003c\/i\u003e cell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Liver Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Liver Fibroblasts from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’ \u003c\/i\u003eonline protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527630344476,"sku":"db-6019","price":637.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687823921436,"sku":"db-6019","price":637.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687823954204,"sku":"db-6019","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-fdeff8eb79.jpg?v=1791766977"},{"product_id":"bks-db-mouse-mammary-microvascular-endothelial-cells","title":"BKS db Mouse Mammary Microvascular Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Mammary Microvascular Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6020\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m1168\"\u003eSuggested Medium: Endothelial Cell Medium \/w Kit – 500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m1168\"\u003e\u003cb\u003eM1168\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Mammary Microvascular Endothelial Cells are isolated from the mammary microvascular of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Mammary Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Mammary Microvascular Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e' Cell Genotype Promise\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003cem\u003eCell Biologics' \u003c\/em\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mammary Microvascular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Mammary Microvascular Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527630410012,"sku":"db-6020","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687823986972,"sku":"db-6020","price":875.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687824019740,"sku":"db-6020","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff9dacf44f.jpg?v=1791766977"},{"product_id":"bks-db-mouse-spleen-fibroblasts","title":"BKS db Mouse Spleen Fibroblasts","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Spleen Fibroblasts\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6022\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m2267\"\u003eSuggested Medium: Fibroblast Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m2267\"\u003e\u003cb\u003eM2267\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Spleen Fibroblasts are isolated from the spleen of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Spleen Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Spleen Fibroblasts are tested for expression of marker using the antibody of anti-FSP1\/S100A4 (Millipore USA) by immunofluorescence staining. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics'\u003c\/i\u003e Cell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics'\u003c\/i\u003e cell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Spleen Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Spleen Fibroblasts from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’ \u003c\/i\u003eonline protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527630475548,"sku":"db-6022","price":637.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687824052508,"sku":"db-6022","price":637.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687824085276,"sku":"db-6022","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-fdeff8eb79.jpg?v=1791766977"},{"product_id":"bks-db-mouse-brain-microvascular-endothelial-cells","title":"BKS db Mouse Brain Microvascular Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Brain Microvascular Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6023\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m1168\"\u003eSuggested Medium: Endothelial Cell Medium \/w Kit – 500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m1168\"\u003e\u003cb\u003eM1168\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Brain Microvascular Endothelial Cells are isolated from the brain microvascular of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Brain Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Brain Microvascular Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e' Cell Genotype Promise\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003cem\u003eCell Biologics' \u003c\/em\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Brain Microvascular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Brain Microvascular Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527630541084,"sku":"db-6023","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687824118044,"sku":"db-6023","price":875.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687824150812,"sku":"db-6023","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff9dacf44f.jpg?v=1791766977"},{"product_id":"bks-db-mouse-cardiac-microvascular-endothelial-cells","title":"BKS db Mouse Cardiac Microvascular Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Cardiac Microvascular Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6024\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m1168\"\u003eSuggested Medium: Endothelial Cell Medium \/w Kit – 500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m1168\"\u003e\u003cb\u003eM1168\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Cardiac Microvascular Endothelial Cells are isolated from the cardiac microvascular of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Cardiac Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Cardiac Microvascular Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e' Cell Genotype Promise\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003cem\u003eCell Biologics' \u003c\/em\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Cardiac Microvascular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Cardiac Microvascular Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527630639388,"sku":"db-6024","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687824183580,"sku":"db-6024","price":875.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687824216348,"sku":"db-6024","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff9dacf44f.jpg?v=1791766977"},{"product_id":"bks-db-mouse-small-intestinal-fibroblasts","title":"BKS db Mouse Small Intestinal Fibroblasts","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Small Intestinal Fibroblasts\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6025\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m2267\"\u003eSuggested Medium: Fibroblast Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m2267\"\u003e\u003cb\u003eM2267\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Small Intestinal Fibroblasts are isolated from the small Intestine of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Small Intestinal Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Small Intestinal Fibroblasts are tested for expression of marker using the antibody of anti-FSP1\/S100A4 (Millipore USA) by immunofluorescence staining. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics'\u003c\/i\u003e Cell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics'\u003c\/i\u003e cell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Small Intestinal Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Small Intestinal Fibroblasts from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’ \u003c\/i\u003eonline protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527630737692,"sku":"db-6025","price":637.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687824249116,"sku":"db-6025","price":700.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687824281884,"sku":"db-6025","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-fdeff8eb79.jpg?v=1791766977"},{"product_id":"bks-db-mouse-prostate-endothelial-cells","title":"BKS db Mouse Prostate Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Prostate Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6206\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m1168\"\u003eSuggested Medium: Endothelial Cell Medium \/w Kit – 500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m1168\"\u003e\u003cb\u003eM1168\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Prostate Endothelial Cells are isolated from the prostate of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Prostate Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Prostate Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e' Cell Genotype Promise\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003cem\u003eCell Biologics' \u003c\/em\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mammary Prostate Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Prostate Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527630803228,"sku":"db-6027","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687824314652,"sku":"db-6027","price":875.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687824347420,"sku":"db-6027","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff9dacf44f.jpg?v=1791766977"},{"product_id":"bks-db-mouse-tracheal-epithelial-cells","title":"BKS db Mouse Tracheal Epithelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Tracheal\u003c\/b\u003e\u003cb\u003e Epithelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6033\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=m6621\"\u003eEpithelial Cell Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m6621\"\u003e\u003cb\u003eM6621\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Tracheal Epithelial Cells are isolated from the trachea of mouse homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Tracheal Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db MouseTracheal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology) or Supernatant (T1 alpha). T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics' \u003c\/i\u003eCell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics' \u003c\/i\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Tracheal Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Tracheal Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent.\u003ci\u003e Cell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics \u003c\/i\u003ewith caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527630967068,"sku":"db-6033","price":775.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687824380188,"sku":"db-6033","price":837.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687824412956,"sku":"db-6033","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-bf490624fc.jpg?v=1791766977"},{"product_id":"bks-db-mouse-kidney-epithelial-cells","title":"BKS db Mouse Kidney Epithelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse \u003c\/b\u003e\u003cb\u003eKidney Epithelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6034\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=m6621\"\u003eEpithelial Cell Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m6621\"\u003e\u003cb\u003eM6621\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Kidney Epithelial Cells are isolated from the kidney of mouse homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Kidney Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Kidney Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology) or Supernatant (T1 alpha). T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics' \u003c\/i\u003eCell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics' \u003c\/i\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Kidney Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Kidney Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent.\u003ci\u003e Cell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics \u003c\/i\u003ewith caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527631032604,"sku":"db-6034","price":775.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687824445724,"sku":"db-6034","price":837.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687824478492,"sku":"db-6034","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-bf490624fc.jpg?v=1791766977"},{"product_id":"bks-db-mouse-mammary-epithelial-cells","title":"BKS db Mouse Mammary Epithelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Mammary\u003c\/b\u003e\u003cb\u003e Epithelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6044\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=m6621\"\u003eEpithelial Cell Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m6621\"\u003e\u003cb\u003eM6621\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Mammary Epithelial Cells are isolated from the mamma of mouse homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Mammary Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Mammary Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology) or Supernatant (T1 alpha). T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics' \u003c\/i\u003eCell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics' \u003c\/i\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Mammary Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Mammary Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent.\u003ci\u003e Cell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics \u003c\/i\u003ewith caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527631065372,"sku":"db-6035","price":775.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687824511260,"sku":"db-6035","price":837.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687824544028,"sku":"db-6035","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-bf490624fc.jpg?v=1791766977"},{"product_id":"bks-db-mouse-ovarian-epithelial-cells","title":"BKS db Mouse Ovarian Epithelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Ovarian\u003c\/b\u003e\u003cb\u003e Epithelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6036\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=m6621\"\u003eEpithelial Cell Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m6621\"\u003e\u003cb\u003eM6621\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Ovarian Epithelial Cells are isolated from the ovarian of mouse homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Ovarian Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Ovarian Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology) or Supernatant (T1 alpha). T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics' \u003c\/i\u003eCell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics' \u003c\/i\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Ovarian Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Ovarian Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent.\u003ci\u003e Cell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics \u003c\/i\u003ewith caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527631163676,"sku":"db-6036","price":775.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687824576796,"sku":"db-6036","price":837.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687824609564,"sku":"db-6036","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-bf490624fc.jpg?v=1791766977"},{"product_id":"bks-db-mouse-pancreatic-epithelial-cells","title":"BKS db Mouse Pancreatic Epithelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Pancreatic\u003c\/b\u003e\u003cb\u003e Epithelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6037\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=m6621\"\u003eEpithelial Cell Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m6621\"\u003e\u003cb\u003eM6621\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Pancreatic Epithelial Cells are isolated from the pancrea of mouse homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Pancreatic Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Pancreatic Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology) or Supernatant (T1 alpha). T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics' \u003c\/i\u003eCell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics' \u003c\/i\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Pancreatic Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Pancreatic Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent.\u003ci\u003e Cell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics \u003c\/i\u003ewith caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527631261980,"sku":"db-6037","price":775.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687824642332,"sku":"db-6037","price":837.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687824675100,"sku":"db-6037","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-bf490624fc.jpg?v=1791766977"},{"product_id":"bks-db-mouse-prostate-epithelial-cells","title":"BKS db Mouse Prostate Epithelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Prostate\u003c\/b\u003e\u003cb\u003e Epithelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6038\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=m6621\"\u003eEpithelial Cell Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m6621\"\u003e\u003cb\u003eM6621\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Prostate Epithelial Cells are isolated from the prostate of mouse homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Prostate Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Prostate Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology) or Supernatant (T1 alpha). T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics' \u003c\/i\u003eCell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics' \u003c\/i\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Prostate Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Prostate Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent.\u003ci\u003e Cell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics \u003c\/i\u003ewith caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527631327516,"sku":"db-6038","price":775.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687824707868,"sku":"db-6038","price":837.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687824740636,"sku":"db-6038","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-bf490624fc.jpg?v=1791766977"},{"product_id":"bks-db-mouse-stomach-epithelial-cells","title":"BKS db Mouse Stomach Epithelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Stomach\u003c\/b\u003e\u003cb\u003e Epithelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6039\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=m6621\"\u003eEpithelial Cell Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m6621\"\u003e\u003cb\u003eM6621\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Stomach Epithelial Cells are isolated from the stomach of mouse homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Stomach Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Stomach Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology) or Supernatant (T1 alpha). T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics' \u003c\/i\u003eCell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics' \u003c\/i\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Stomach Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Stomach Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent.\u003ci\u003e Cell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics \u003c\/i\u003ewith caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527631393052,"sku":"db-6039","price":775.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687824773404,"sku":"db-6039","price":837.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687824806172,"sku":"db-6039","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-bf490624fc.jpg?v=1791766977"},{"product_id":"bks-db-mouse-thyroid-epithelial-cells","title":"BKS db Mouse Thyroid Epithelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Thyroid\u003c\/b\u003e\u003cb\u003e Epithelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6040\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=m6621\"\u003eEpithelial Cell Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m6621\"\u003e\u003cb\u003eM6621\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Thyroid Epithelial Cells are isolated from the thyroid of mouse homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Thyroid Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Thyroid Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology) or Supernatant (T1 alpha). T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics' \u003c\/i\u003eCell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics' \u003c\/i\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Thyroid Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Thymus Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent.\u003ci\u003e Cell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics \u003c\/i\u003ewith caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527631491356,"sku":"db-6040","price":775.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687824871708,"sku":"db-6040","price":837.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687824904476,"sku":"db-6040","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-bf490624fc.jpg?v=1791766977"},{"product_id":"bks-db-mouse-prostate-smooth-muscle-cells","title":"BKS db Mouse Prostate Smooth Muscle Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Prostate Muscle Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6041\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m2268\"\u003eSuggested Medium: Smooth Muscle Cell Medium \/w Kit –500 ML\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m2268\"\u003e\u003cb\u003eM2268\u003c\/b\u003e\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Prostate Muscle Cells are isolated from the prostate of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Prostate Smooth Muscle Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics\u003c\/i\u003e’ Culture Complete Growth Medium generally for 3-7 days. Prior to shipping, cells at passage 1 are detached from the culture flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Prostate Muscle Cells from \u003ci\u003eCell Biologics\u003c\/i\u003eare characterized by immunofluorescent method with antibodies to α-smooth muscle actin. BKS db Mouse Prostate Smooth Muscle Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments (or cells may be expanded for 2-4 passages at a split ratio of 1:2) under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics'\u003c\/i\u003e Cell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics \u003c\/i\u003ehas established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics'\u003c\/i\u003e cell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Prostate Muscle Cells can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Prostate Muscle Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527631720732,"sku":"db-6041","price":725.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687825002780,"sku":"db-6041","price":787.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687825035548,"sku":"db-6041","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-54bed8ead2.jpg?v=1791767087"},{"product_id":"bks-db-mouse-bone-marrow-mesenchymal-stem-cells","title":"BKS db Mouse Bone Marrow Mesenchymal Stem Cells","description":"\u003cp\u003eBKS db Mouse Bone Marrow Mesenchymal Stem Cells - BKS db Mouse primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527631819036,"sku":"db-6043","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687825068316,"sku":"db-6043","price":875.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687825101084,"sku":"db-6043","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-4dd4a9b8a9.jpg?v=1791766978"},{"product_id":"bks-db-mouse-liver-epithelial-cells","title":"BKS db Mouse Liver Epithelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse \u003c\/b\u003e\u003cb\u003eLiver\u003c\/b\u003e\u003cb\u003e Epithelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6044\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=m6621\"\u003eEpithelial Cell Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m6621\"\u003e\u003cb\u003eM6621\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Liver Epithelial Cells are isolated from the liver of mouse homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Liver Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Liver Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology) or Supernatant (T1 alpha). T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics' \u003c\/i\u003eCell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics' \u003c\/i\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Liver Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Liver Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent.\u003ci\u003e Cell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics \u003c\/i\u003ewith caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527631884572,"sku":"db-6044","price":775.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687825133852,"sku":"db-6044","price":837.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687825166620,"sku":"db-6044","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-bf490624fc.jpg?v=1791766977"},{"product_id":"bks-db-mouse-cholangiocytes","title":"BKS db Mouse Cholangiocytes","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Cholangiocytes\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6044C\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=m6621\"\u003eEpithelial Cell Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m6621\"\u003e\u003cb\u003eM6621\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Cholangiocytes are isolated from the cholangio of mouse homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Cholangiocytes are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Cholangiocytes are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology) or Supernatant (T1 alpha). T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics' \u003c\/i\u003eCell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics' \u003c\/i\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Cholangiocytes can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Cholangiocytes from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent.\u003ci\u003e Cell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics \u003c\/i\u003ewith caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527631950108,"sku":"db-6044C","price":775.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687825199388,"sku":"db-6044C","price":837.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687825232156,"sku":"db-6044C","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-bf490624fc.jpg?v=1791766977"},{"product_id":"bks-db-mouse-spleen-epithelial-cells","title":"BKS db Mouse Spleen Epithelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Spleen\u003c\/b\u003e\u003cb\u003e Epithelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6045\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=m6621\"\u003eEpithelial Cell Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m6621\"\u003e\u003cb\u003eM6621\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Spleen Epithelial Cells are isolated from the spleen of mouse homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Spleen Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Spleen Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology) or Supernatant (T1 alpha). T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics' \u003c\/i\u003eCell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics' \u003c\/i\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Spleen Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Spleen Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent.\u003ci\u003e Cell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics \u003c\/i\u003ewith caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527632015644,"sku":"db-6045","price":775.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687825264924,"sku":"db-6045","price":837.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687825297692,"sku":"db-6045","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-bf490624fc.jpg?v=1791766977"},{"product_id":"bks-db-mouse-esophageal-epithelial-cells","title":"BKS db Mouse Esophageal Epithelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Esophageal Epithelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6046\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=m6621\"\u003eEpithelial Cell Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m6621\"\u003e\u003cb\u003eM6621\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Esophageal Epithelial Cells are isolated from the esophagea of mouse homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Esophageal Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Esophageal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology) or Supernatant (T1 alpha). T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics' \u003c\/i\u003eCell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics' \u003c\/i\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Esophageal Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Esophageal Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent.\u003ci\u003e Cell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics \u003c\/i\u003ewith caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527632081180,"sku":"db-6046","price":775.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687825330460,"sku":"db-6046","price":837.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687825363228,"sku":"db-6046","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-bf490624fc.jpg?v=1791766977"},{"product_id":"bks-db-mouse-colonic-epithelial-cells","title":"BKS db Mouse Colonic Epithelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse \u003c\/b\u003e\u003cb\u003eColonic\u003c\/b\u003e\u003cb\u003e Epithelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6047\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=m6621\"\u003eEpithelial Cell Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m6621\"\u003e\u003cb\u003eM6621\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Colonic Epithelial Cells are isolated from the colon of mouse homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Colonic Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Colonic Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology) or Supernatant (T1 alpha). T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics' \u003c\/i\u003eCell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics' \u003c\/i\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Colonic Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Colonic Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent.\u003ci\u003e Cell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics \u003c\/i\u003ewith caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527632146716,"sku":"db-6047","price":775.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687825395996,"sku":"db-6047","price":837.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687825428764,"sku":"db-6047","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-bf490624fc.jpg?v=1791766977"},{"product_id":"bks-db-mouse-corneal-epithelial-cells","title":"BKS db Mouse Corneal Epithelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Corneal Epithelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6048\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=m6621\"\u003eEpithelial Cell Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m6621\"\u003e\u003cb\u003eM6621\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Corneal Epithelial Cells are isolated from the cornea of mouse homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Corneal Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Corneal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology) or Supernatant (T1 alpha). T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics' \u003c\/i\u003eCell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics' \u003c\/i\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Corneal Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Corneal Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent.\u003ci\u003e Cell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics \u003c\/i\u003ewith caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527632212252,"sku":"db-6048","price":775.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687825461532,"sku":"db-6048","price":837.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687825494300,"sku":"db-6048","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-bf490624fc.jpg?v=1791766977"},{"product_id":"bks-db-mouse-cardiac-fibroblasts","title":"BKS db Mouse Cardiac Fibroblasts","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Cardiac \u003c\/b\u003e\u003cb\u003eFibroblasts\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6049\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m2267\"\u003eSuggested Medium: Fibroblast Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m2267\"\u003e\u003cb\u003eM2267\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Cardiac Fibroblasts are isolated from the cardiac of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Cardiac Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Cardiac Fibroblasts are tested for expression of marker using the antibody of anti-FSP1\/S100A4 (Millipore USA) by immunofluorescence staining. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics'\u003c\/i\u003e Cell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics'\u003c\/i\u003e cell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Cardiac Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Cardiac Fibroblasts from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’ \u003c\/i\u003eonline protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527632343324,"sku":"db-6049","price":637.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687825527068,"sku":"db-6049","price":700.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687825559836,"sku":"db-6049","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-fdeff8eb79.jpg?v=1791766977"},{"product_id":"bks-db-mouse-coronary-artery-fibroblasts","title":"BKS db Mouse Coronary Artery Fibroblasts","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Coronary Artery Fibroblasts\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6049CO\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m2267\"\u003eSuggested Medium: Fibroblast Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m2267\"\u003e\u003cb\u003eM2267\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Coronary Artery Fibroblasts are isolated from the coronary artery of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Coronary Artery Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Coronary Artery Fibroblasts are tested for expression of marker using the antibody of anti-FSP1\/S100A4 (Millipore USA) by immunofluorescence staining. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics'\u003c\/i\u003e Cell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics'\u003c\/i\u003e cell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Coronary Artery Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Coronary Artery Fibroblasts from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’ \u003c\/i\u003eonline protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527632408860,"sku":"db-6049CO","price":637.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687825592604,"sku":"db-6049CO","price":700.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687825625372,"sku":"db-6049CO","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-fdeff8eb79.jpg?v=1791766977"},{"product_id":"bks-db-mouse-small-intestinal-epithelial-cells","title":"BKS db Mouse Small Intestinal Epithelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Small Intestinal\u003c\/b\u003e\u003cb\u003e Epithelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6051\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=m6621\"\u003eEpithelial Cell Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m6621\"\u003e\u003cb\u003eM6621\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Small Intestinal Epithelial Cells are isolated from the small intestine of mouse homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Small Intestinal Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Small Intestinal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology) or Supernatant (T1 alpha). T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics' \u003c\/i\u003eCell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics' \u003c\/i\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Small Intestinal Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Small Intestinal Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent.\u003ci\u003e Cell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics \u003c\/i\u003ewith caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527632474396,"sku":"db-6051","price":775.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687825658140,"sku":"db-6051","price":837.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687825690908,"sku":"db-6051","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-bf490624fc.jpg?v=1791766977"},{"product_id":"bks-db-mouse-aortic-endothelial-cells","title":"BKS db Mouse Aortic Endothelial cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Aortic Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6052\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m1168\"\u003eSuggested Medium: Endothelial Cell Medium \/w Kit – 500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m1168\"\u003e\u003cb\u003eM1168\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Aortic Endothelial Cells are isolated from the aorta of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Aortic Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Aortic Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e' Cell Genotype Promise\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003cem\u003eCell Biologics' \u003c\/em\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Aortic Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Aortic Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527632539932,"sku":"db-6052","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687825723676,"sku":"db-6052","price":875.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687825756444,"sku":"db-6052","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff9dacf44f.jpg?v=1791766977"},{"product_id":"bks-db-mouse-alveolar-epithelial-cells","title":"BKS db Mouse Alveolar Epithelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Alveolar Epithelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6053\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=m6621\"\u003eEpithelial Cell Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m6621\"\u003e\u003cb\u003eM6621\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Alveolar Epithelial Cells are isolated from the lung of mouse homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Alveolar Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Alveolar Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology) or Supernatant (T1 alpha). T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics' \u003c\/i\u003e\u003c\/b\u003e\u003cb\u003eCell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics' \u003c\/i\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Alveolar Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Alveolar Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent.\u003ci\u003e Cell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics \u003c\/i\u003ewith caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527632605468,"sku":"db-6053","price":775.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687825789212,"sku":"db-6053","price":837.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687825821980,"sku":"db-6053","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-bf490624fc.jpg?v=1791766977"},{"product_id":"bks-db-mouse-small-intestinal-microvascular-endothelial-cells","title":"BKS db Mouse Small Intestinal Microvascular Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Small Intestinal Microvascular Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6054\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m1168\"\u003eSuggested Medium: Endothelial Cell Medium \/w Kit – 500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m1168\"\u003e\u003cb\u003eM1168\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Small Intestinal Microvascular Endothelial Cells are isolated from the small intestinal microvascular of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Small Intestinal Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Small Intestinal Microvascular Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e' Cell Genotype Promise\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003cem\u003eCell Biologics' \u003c\/em\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mammary Small Intestinal Microvascular Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Small Intestinal Microvascular Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527632638236,"sku":"db-6054","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687825854748,"sku":"db-6054","price":875.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687825887516,"sku":"db-6054","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff9dacf44f.jpg?v=1791766977"},{"product_id":"bks-db-mouse-intestinal-mesenteric-vascular-endothelial-cells","title":"BKS db Mouse Intestinal Mesenteric Vascular Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Intestinal Mesenteric Vascular Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6055\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m1168\"\u003eSuggested Medium: Endothelial Cell Medium \/w Kit – 500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m1168\"\u003e\u003cb\u003eM1168\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Intestinal Mesenteric Vascular Endothelial Cells are isolated from the intestinal mesenteric vascular of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Intestinal Mesenteric Vascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Intestinal Mesenteric Vascular Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e' Cell Genotype Promise\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003cem\u003eCell Biologics' \u003c\/em\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Intestinal Mesenteric Vascular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Intestinal Mesenteric Vascular Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527632703772,"sku":"db-6055","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687827296540,"sku":"db-6055","price":875.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687827329308,"sku":"db-6055","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff9dacf44f.jpg?v=1791766977"},{"product_id":"bks-db-mouse-spleen-endothelial-cells","title":"BKS db Mouse Spleen Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Spleen Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6057\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m1168\"\u003eSuggested Medium: Endothelial Cell Medium \/w Kit – 500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m1168\"\u003e\u003cb\u003eM1168\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Spleen Endothelial Cells are isolated from the spleen of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Spleen Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Spleen Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e' Cell Genotype Promise\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003cem\u003eCell Biologics' \u003c\/em\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mammary Spleen Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Spleen Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527632736540,"sku":"db-6057","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687827362076,"sku":"db-6057","price":875.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687827394844,"sku":"db-6057","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff9dacf44f.jpg?v=1791766977"},{"product_id":"bks-db-mouse-thyroid-endothelial-cells","title":"BKS db Mouse Thyroid Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Thyroid Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6058\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m1168\"\u003eSuggested Medium: Endothelial Cell Medium \/w Kit – 500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m1168\"\u003e\u003cb\u003eM1168\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Thyroid Endothelial Cells are isolated from the thyroid of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Thyroid Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Thyroid Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e' Cell Genotype Promise\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003cem\u003eCell Biologics' \u003c\/em\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mammary Thyroid Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Thyroid Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527632802076,"sku":"db-6058","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687827427612,"sku":"db-6058","price":875.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687827460380,"sku":"db-6058","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff9dacf44f.jpg?v=1791766977"},{"product_id":"bks-db-mouse-pulmonary-artery-endothelial-cells","title":"BKS db Mouse Pulmonary Artery Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Pulmonary Artery Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6059\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m1168\"\u003eSuggested Medium: Endothelial Cell Medium \/w Kit – 500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m1168\"\u003e\u003cb\u003eM1168\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Pulmonary Artery Endothelial Cells are isolated from the pulmonary artery of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Pulmonary Artery Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Pulmonary Artery Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e' Cell Genotype Promise\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003cem\u003eCell Biologics' \u003c\/em\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mammary Pulmonary Artery Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Pulmonary Artery Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527632965916,"sku":"db-6059","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687827493148,"sku":"db-6059","price":875.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687827525916,"sku":"db-6059","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff9dacf44f.jpg?v=1791766977"},{"product_id":"bks-db-mouse-pulmonary-vein-endothelial-cells","title":"BKS db Mouse Pulmonary Vein Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Pulmonary Vein Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6060\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m1168\"\u003eSuggested Medium: Endothelial Cell Medium \/w Kit – 500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m1168\"\u003e\u003cb\u003eM1168\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Pulmonary Vein Endothelial Cells are isolated from the pulmonary vein of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Pulmonary Vein Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Pulmonary Vein Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e' Cell Genotype Promise\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003cem\u003eCell Biologics' \u003c\/em\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mammary Pulmonary Vein Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Pulmonary Vein Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527633031452,"sku":"db-6060","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687827558684,"sku":"db-6060","price":875.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687827591452,"sku":"db-6060","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff9dacf44f.jpg?v=1791766977"},{"product_id":"bks-db-mouse-dermal-microvascular-endothelial-cells","title":"BKS db Mouse Dermal Microvascular Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Dermal Microvascular Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6064\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m1168\"\u003eSuggested Medium: Endothelial Cell Medium \/w Kit – 500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m1168\"\u003e\u003cb\u003eM1168\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Dermal Microvascular Endothelial Cells are isolated from the dermal microvascular of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Dermal Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Dermal Microvascular Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e' Cell Genotype Promise\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003cem\u003eCell Biologics' \u003c\/em\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Dermal Microvascular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Dermal Microvascular Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527633064220,"sku":"db-6064","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687827624220,"sku":"db-6064","price":875.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687827656988,"sku":"db-6064","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff9dacf44f.jpg?v=1791766977"},{"product_id":"bks-db-mouse-dermal-lymphatic-endothelial-cells","title":"BKS db Mouse Dermal Lymphatic Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Dermal Lymphatic Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6064L\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m1168\"\u003eSuggested Medium: Endothelial Cell Medium \/w Kit – 500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m1168\"\u003e\u003cb\u003eM1168\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Dermal Lymphatic Endothelial Cells are isolated from the dermal lymphatic of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Dermal Lymphatic Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Dermal Lymphatic Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e' Cell Genotype Promise\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003cem\u003eCell Biologics' \u003c\/em\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Dermal Lymphatic Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Dermal Lymphatic Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527633129756,"sku":"db-6064L","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687827689756,"sku":"db-6064L","price":875.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687827722524,"sku":"db-6064L","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff9dacf44f.jpg?v=1791766977"},{"product_id":"bks-db-mouse-retinal-microvascular-endothelial-cells","title":"BKS db Mouse Retinal Microvascular Endothelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Retinal Microvascular Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6065\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m1168\"\u003eSuggested Medium: Endothelial Cell Medium \/w Kit – 500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m1168\"\u003e\u003cb\u003eM1168\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Retinal Microvascular Endothelial Cells are isolated from the retinal microvascular of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Retinal Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Retinal Microvascular Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e' Cell Genotype Promise\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003cem\u003eCell Biologics' \u003c\/em\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mammary Retinal Microvascular Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Retinal Microvascular Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003cu\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/u\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527633195292,"sku":"db-6065","price":812.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687827755292,"sku":"db-6065","price":875.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687827788060,"sku":"db-6065","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-ff9dacf44f.jpg?v=1791766977"},{"product_id":"bks-db-mouse-dermal-epithelial-cells","title":"BKS db Mouse Dermal Epithelial Cells","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Dermal Epithelial Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6066\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=m6621\"\u003eEpithelial Cell Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m6621\"\u003e\u003cb\u003eM6621\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Dermal Epithelial Cells are isolated from the derma of mouse homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Dermal Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Dermal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology) or Supernatant (T1 alpha). T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics' \u003c\/i\u003eCell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics' \u003c\/i\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Dermal Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Dermal Epithelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent.\u003ci\u003e Cell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics \u003c\/i\u003ewith caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527633228060,"sku":"db-6066","price":775.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687827820828,"sku":"db-6066","price":837.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687827853596,"sku":"db-6066","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-bf490624fc.jpg?v=1791766977"},{"product_id":"bks-db-mouse-epidermal-keratinocytes","title":"BKS db Mouse Epidermal Keratinocytes","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Epithelial Keratinocytes\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6066K\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eSuggested Medium: \u003ca href=\"\/search?q=m6621\"\u003eEpithelial Cell Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m6621\"\u003e\u003cb\u003eM6621\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Epidermal Keratinocytes are isolated from the epidermal keratin of mouse homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Epidermal Keratinocytes Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Epidermal Keratinocytes are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology) or Supernatant (T1 alpha). T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics' \u003c\/i\u003eCell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics' \u003c\/i\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Epidermal Keratinocytes can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Epidermal Keratinocytes Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent.\u003ci\u003e Cell Biologics’ \u003c\/i\u003eTerms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics \u003c\/i\u003ewith caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’\u003c\/i\u003e online protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527633293596,"sku":"db-6066K","price":775.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687827886364,"sku":"db-6066K","price":837.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687827919132,"sku":"db-6066K","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-bf490624fc.jpg?v=1791766977"},{"product_id":"bks-db-mouse-dermal-fibroblasts-adult","title":"BKS db Mouse Dermal Fibroblasts-adult","description":"\u003cp\u003e\u003cb\u003eBKS db Mouse Dermal Fibroblasts-adult\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003cb\u003edb-6067\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"\/search?q=m2267\"\u003eSuggested Medium: Fibroblast Medium \/w Kit –500 ml\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. \u003ca href=\"\/search?q=m2267\"\u003e\u003cb\u003eM2267\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Description\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Dermal Fibroblasts-adult are isolated from the dermal of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic. BKS db Mouse Dermal Fibroblasts-adult are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Dermal Fibroblasts-adult are tested for expression of marker using the antibody of anti-FSP1\/S100A4 (Millipore USA) by immunofluorescence staining. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\u003cp\u003e\u003cb\u003e\u003ci\u003eCell Biologics'\u003c\/i\u003e Cell Genotype Promise\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003ci\u003eCell Biologics'\u003c\/i\u003e cell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that \u003ci\u003eCell Biologics'\u003c\/i\u003e cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLaboratory Applications\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Dermal Fibroblasts-adult can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAuthorized Uses of \u003ci\u003eCell Biologics’\u003c\/i\u003e Products\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eBKS db Mouse Dermal Fibroblasts-adult from \u003ci\u003eCell Biologics\u003c\/i\u003e are distributed for laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any \u003ci\u003eCell Biologics’\u003c\/i\u003e cells or products from the purchaser to other markets, organizations or individuals is prohibited by \u003ci\u003eCell Biologics\u003c\/i\u003e without the company’s written consent. \u003ci\u003eCell Biologics’\u003c\/i\u003e Terms and Conditions must be accepted before submitting an order. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eDisclaimer\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWarranty and Liability\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eCell Biologics’\u003c\/i\u003e guarantee applies only to your purchase of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cells with \u003ci\u003eCell Biologics’\u003c\/i\u003e Media and Coating Solution for appropriate cell culture and cell testing following \u003ci\u003eCell Biologics’ \u003c\/i\u003eonline protocols within 35 days from the date of product delivery.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527633359132,"sku":"db-6067","price":637.5,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":54687827951900,"sku":"db-6067","price":637.5,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":54687827984668,"sku":"db-6067","price":1112.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-fdeff8eb79.jpg?v=1791766977"}],"url":"https:\/\/jkpgye-36.myshopify.com\/collections\/bks-db-db-mouse-cells.oembed?page=2","provider":"Cell Biologics","version":"1.0","type":"link"}